دورية أكاديمية
Ordered mesoporous materials containing Mucor Miehei Lipase as biocatalyst for transesterification reaction
| العنوان: | Ordered mesoporous materials containing Mucor Miehei Lipase as biocatalyst for transesterification reaction |
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| المؤلفون: | Jacoby, J., Pasc, Andreea, Carteret, C., Dupire, F., Stebe, Marie-José, Coupard, V., Blin, Jean-Luc |
| المساهمون: | Structure et Réactivité des Systèmes Moléculaires Complexes (SRSMC), Institut de Chimie - CNRS Chimie (INC-CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), IFP Energies nouvelles (IFPEN) |
| المصدر: | ISSN: 1359-5113 ; Process Biochemistry ; https://hal.univ-lorraine.fr/hal-01494084Test ; Process Biochemistry, 2013, 48 (5-6), pp.831-837. ⟨10.1016/j.procbio.2013.04.003⟩. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2013 |
| المجموعة: | Université de Lorraine: HAL |
| مصطلحات موضوعية: | Lipase, Ordered mesoporous silica, Immobilization, Biocatalyst, Methanolysis, Colza oil, [CHIM]Chemical Sciences |
| الوصف: | International audience ; Herein, we report the design of a biocatalyst by the immobilization of Mucor Miehei Lipase (Mm-L) onto mesoporous silica materials. Supports with different pore diameters have been considered. Infrared spectroscopy was used to determine the adsorption isotherms in different pH conditions. Then, the biocatalyst was tested for the methanolysis of colza oil. The production of methyl esters was monitored over time by gas chromatography coupled to a mass spectrometer. The results show that to reach maximum performance of the biocatalyst, a certain amount of water is required (5 wt.%). By using ratio lower than the stoichiometry (1:1), the methanol conversion is completed and high transesterification yields could be obtained even in the absence of non polar solvents (i.e. hexane). Herein, the lipase uses the fatty substrate as lipophilic interface required for the opening of the active site of the enzyme. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-01494084; https://hal.univ-lorraine.fr/hal-01494084Test |
| DOI: | 10.1016/j.procbio.2013.04.003 |
| الإتاحة: | https://doi.org/10.1016/j.procbio.2013.04.003Test https://hal.univ-lorraine.fr/hal-01494084Test |
| رقم الانضمام: | edsbas.2C1A1088 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.procbio.2013.04.003 |
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