دورية أكاديمية
Regulation of KCNQ1/KCNE1 by β-catenin.
| العنوان: | Regulation of KCNQ1/KCNE1 by β-catenin. |
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| المؤلفون: | Wilmes J., Haddad Tóvolli R., Alesutan I., Munoz C, Sopjani M., Pelzl L., Bogatikov E., Fedele G., Seebohm G., Föller M., Lang F., FAGGIO, Caterina |
| المساهمون: | Wilmes J., Haddad-Tóvolli R., Alesutan I., Munoz C, Sopjani M., Pelzl L., Bogatikov E., Fedele G., Faggio C., Seebohm G., Föller M., Lang F. |
| سنة النشر: | 2012 |
| المجموعة: | Università degli Studi di Messina: IRIS |
| مصطلحات موضوعية: | IK, cardiac repolarization, vesicle trafficking, cardiac hypertrophy |
| الوصف: | β-catenin, a multifunctional protein expressed in all tissues including the heart stimulates the expression of several genes important for cell proliferation. Signaling involving ß-catenin participates in directing cardiac development and in the pathophysiology of cardiac hypertrophy. Nothing is known, however, on the role of β-catenin in the regulation of cardiac ion channels. The present study explored the functional interaction of β-catenin and KCNE1/KCNQ1, the K(+) channel complex underlying the slowly activating outwardly rectifying K(+) current. To this end, KCNE1/KCNQ1 was expressed in Xenopus oocytes with and without β-catenin and the depolarization (up to + 80 mV) induced current (I(Ks)) was determined using the two-electrode voltage clamp. As a result, β-catenin enhanced I(Ks) by 30%. The effect of β-catenin on I(Ks) was not affected by actinomycin D (10 μM), an inhibitor of transcription, indicating that β-catenin was not effective as transcription factor. Confocal microscopy revealed that β-catenin enhanced the KCNE1/KCNQ1 protein abundance in the cell membrane. Exposure of the oocytes to brefeldin A (5 μM), an inhibitor of vesicle insertion, was followed by a decline of I(Ks), which was then similar in oocytes expressing KCNE1/KCNQ1 together with β-catenin and in oocytes expressing KCNE1/KCNQ1 alone. In conclusion, β-catenin enhances I(Ks) by increasing the KCNE1/KCNQ1 protein abundance in the cell membrane, an effect requiring vesicle insertion into the cell membrane. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/22583083; info:eu-repo/semantics/altIdentifier/wos/WOS:000305423900002; volume:29; firstpage:87; lastpage:94; numberofpages:8; journal:MOLECULAR MEMBRANE BIOLOGY; http://hdl.handle.net/11570/1967821Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84862561564 |
| DOI: | 10.3109/09687688.2012.678017 |
| الإتاحة: | https://doi.org/10.3109/09687688.2012.678017Test http://hdl.handle.net/11570/1967821Test |
| رقم الانضمام: | edsbas.74BDBACF |
| قاعدة البيانات: | BASE |
| DOI: | 10.3109/09687688.2012.678017 |
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