المؤلفون: Thiago Stocco, Eliane Antonioli, Conceição Elias, Bruno Rodrigues, Idália Siqueira, Mario Ferretti, Fernanda Marciano, Anderson Lobo

المصدر: Materials; Volume 12; Issue 6; Pages: 849

مصطلحات موضوعية: osteochondral regeneration, nanocomposites, porous scaffolds, carbon nanotubes, PDLLA, hydroxyapatite, chondrocyte

الوصف: Treatment of articular cartilage lesions remains an important challenge. Frequently the bone located below the cartilage is also damaged, resulting in defects known as osteochondral lesions. Tissue engineering has emerged as a potential approach to treat cartilage and osteochondral defects. The principal challenge of osteochondral tissue engineering is to create a scaffold with potential to regenerate both cartilage and the subchondral bone involved, considering the intrinsic properties of each tissue. Recent nanocomposites based on the incorporation of nanoscale fillers into polymer matrix have shown promising results for the treatment of osteochondral defects. In this present study, it was performed using the recently developed methodologies (electrodeposition and immersion in simulated body fluid) to obtain porous superhydrophilic poly(d,l-lactic acid)/vertically aligned carbon nanotubes/nanohydroxyapatite (PDLLA/VACNT-O:nHAp) nanocomposite scaffolds, to analyze cell behavior and gene expression of chondrocytes, and then assess the applicability of this nanobiomaterial for osteochondral regenerative medicine. The results demonstrate that PDLLA/VACNT-O:nHAp nanocomposite supports chondrocytes adhesion and decreases type I Collagen mRNA expression. Therefore, these findings suggest the possibility of novel nanobiomaterial as a scaffold for osteochondral tissue engineering applications.

وصف الملف: application/pdf

العلاقة: https://dx.doi.org/10.3390/ma12060849Test

الإتاحة: https://doi.org/10.3390/ma12060849Test

المؤلفون: Pereira, D. R., Canadas, R. F., Silva-Correia, J., da Silva Morais, A., Oliveira, M. B., Dias, I. R., Mano, J. F., Marques, A. P., Reis, R. L., Oliveira, J. M.

مصطلحات موضوعية: bilayered hydrogel composites, Gellan-gum, hydroxyapatite, Injectable biomaterials, orthotopic knee model, Osteochondral regeneration, Science & Technology

الوصف: Multilayer systems capable of simultaneous dual tissue formation are crucial for regeneration of the osteochondral (OC) unit. Despite the tremendous effort in the field there is still no widely accepted system that stands out in terms of superior OC regeneration. Herein, we developed bilayered hydrogel composites (BHC) combining two structurally stratified layers fabricated from naturally derived and synthetic polymers, gellan-gum (GG) and hydroxyapatite (HAp), respectively. Two formulations were made from either low acyl GG (LAGG) alone or in combination with high acyl GG (HAGG) for the cartilage-like layer. Four bone-like layers were made of LAGG incorporating different ratios of hydroxyapatite (HAp). BHC were assembled in one single construct resulting in eight distinct bilayered constructs. Architectural observations by stereomicroscope and micro-CT (μ-CT) demonstrated a connected stratified structure with good ceramic dispersion within the bone-like layer. Swelling and degradation tests as well mechanical analyse showed a stable viscoelastic construct under dynamic forces. In-vitro studies by encapsulating rabbit's chondrocytes and osteoblasts in the respective layers showed the cytocompatibility of the BHC. Further studies comprising subcutaneous implantation in mice displayed a weak immune response after four weeks. OC orthotopic defects in the rabbit's knee were created and injected with the acellular BHC. OC tissue was regenerated four weeks after implantation as confirmed by cartilaginous and bony tissue formation assessed by histologic staining and μ-CT analysis. The successful fabrication of injectable BHC and their in-vitro and in-vivo performance may be seen as advanced engineered platforms to treat the challenging OC defects. ; This material is based on works supported by the Portuguese Foundation for Science and Technology (FCT) under the OsteoCart project (PTDC/CTM-BPC/115977/2009) and for the M-ERA-NET/0001/2014 project. The authors are grateful to Teresa Oliveira for the assistance with ...

وصف الملف: application/pdf

العلاقة: info:eu-repo/grantAgreement/FCT/5876-PPCDTI/115977/PT; info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F81356%2F2011/PT; https://www.sciencedirect.com/science/article/pii/S2352940718301616Test; Pereira D. R., Canadas R. F., Silva-Correia J., da Silva Morais A., Oliveira M. B., Dias I. R., Mano J. F., Marques A. P., Reis R. L., Oliveira J. M. Injectable gellan-gum/hydroxyapatite-based bilayered hydrogel composites for osteochondral tissue regeneration, Applied Materials Today, Vol. 12, pp. 309-321, doi:10.1016/j.apmt.2018.06.005, 2018; http://hdl.handle.net/1822/56299Test

الإتاحة: https://doi.org/10.1016/j.apmt.2018.06.005Test
http://hdl.handle.net/1822/56299Test

المؤلفون: AMADORI, SOFIA, PANZAVOLTA, SILVIA, BIGI, ADRIANA, Torricelli, P., Parrilli, A., Fini, M.

المساهمون: Amadori, S., Torricelli, P., Panzavolta, S., Parrilli, A., Fini, M., Bigi, A.

مصطلحات موضوعية: co-culture, gelatin, hydroxyapatite, multi-layered scaffold, osteochondral regeneration

الوصف: A promising strategy for osteochondral interface regeneration consists in the development of hybrid scaffolds, composed of distinct but integrated layers able to mimic the different regions of cartilage and bone. We developed multi-layered scaffolds by assembling a gelatin layer with layers containing different amounts of gelatin and hydroxyapatite nanocrystals, and using a gelatin solution (as a glue) to stick layers together. The scaffolds exhibit a high, interconnected porosity and mechanical properties varying with composition along the thickness of the scaffolds up to values of compressive stress and modulus of about 1 and 14 MPa, respectively. In vitro tests demonstrate that the different layers of the scaffolds promote chondrogenic and osteogenic differentiation of Human Mesenchimal Stem Cells (hMSC).

وصف الملف: STAMPA

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/26126665; info:eu-repo/semantics/altIdentifier/wos/WOS:000363688300007; volume:15; issue:11; firstpage:1535; lastpage:1545; numberofpages:11; journal:MACROMOLECULAR BIOSCIENCE; http://hdl.handle.net/11585/520001Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84946478526

الإتاحة: https://doi.org/10.1002/mabi.201500165Test
http://hdl.handle.net/11585/520001Test

المؤلفون: Tampieri A, Sprio S, Sandri M, Campodoni E, Ruffini A, Mengozzi L, Panseri S

المصدر: Amsterdam: Elsevier, 2021
info:cnr-pdr/source/autori:Tampieri A, Sprio S, Sandri M, Campodoni E, Ruffini A, Mengozzi L, Panseri S/titolo:Unconventional, nature-inspired approaches to develop bioceramics for regenerative medicine/titolo_volume:/curatori_volume:/editore: /anno:2021

مصطلحات موضوعية: Biomineralization, Biomorphic transformation, Load-bearing bone, Periodontal regeneration, 3-D scaffold, Ion doping, Bioactive ceramics, Osteochondral regeneration, Bone regeneration, Hydroxyapatite, Nanomedicine, Calcium phosphates, Biomimetics, Regenerative medicine, Magnetic hydroxyapatite

الوصف: Materials science researchers and engineers are exploring the complex domain of biomaterials, as it requires consideration of biocompatibility and, in the frame of Regenerative Medicine, assessment on bioactivity, bio-reabsorbability and ability to be integrated and remodeled. Such a research field requires interdisciplinary approaches and robust knowledge of chemistry, biology and mechanics, in order to cope with the complex chemical composition, multiscale structure and biological functions of the organ to be replaced/regenerated. In fact, after decades of attempts to develop biomaterials able to effectively regenerate tissues/organs, most of them failed in their task and today the need of a radically new approach is commonly recognized. In this scenario, the article describes new frontiers in biomaterial development, fed by novel nature-inspired approaches, and able to circumvent the limitations of the current ceramic technology. Thus it will be illustrated how the biomineralization process, by which nature builds innumerable biologic structures, could be used to obtain scaffolds for regeneration of bone and also complex anatomical regions such as the osteochondral and periodontal tissues. Then the article will show recent developments for load-bearing scaffolds fabrication by using a revolutionary biomorphic transformation process, able to convert a natural hierarchically organized structure in fully ceramic and bioactive devices retaining the original 3-D multiscale structure and the unique damage tolerant performance. Finally, it will be shown how such new bio-devices can be powered by functionalization with magnetic doping and/or nanoparticles, to obtain on-demand activation of bio-functionalities. Particularly, the recent development of a biocompatible, bioactive superparamagnetic apatite is described, as a tool very promising to apply novel safer therapies in nanomedicine and to boost the endogenous potential of debilitated patients.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f76a394b89a757c84695099b83950708Test
https://doi.org/10.1016/b978-0-12-803581-8.12102-2Test

المؤلفون: Joaquim M. Oliveira, Raphaël F. Canadas, Rui L. Reis, João F. Mano, Isabel R. Dias, Mariana B. Oliveira, Joana Silva-Correia, Diana Pereira, Alexandra P. Marques, A. da Silva Morais

المساهمون: Universidade do Minho

المصدر: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP

مصطلحات موضوعية: Gellan-gum, 0206 medical engineering, 02 engineering and technology, Osteochondral regeneration, chemistry.chemical_compound, Hydrogel composite, medicine, General Materials Science, Tissue formation, Bony tissue, chemistry.chemical_classification, Science & Technology, Injectable biomaterials, Chemistry, Regeneration (biology), orthotopic knee model, hydroxyapatite, Polymer, 021001 nanoscience & nanotechnology, 020601 biomedical engineering, Gellan gum, Subcutaneous implantation, bilayered hydrogel composites, Swelling, medicine.symptom, 0210 nano-technology, Biomedical engineering

الوصف: Multilayer systems capable of simultaneous dual tissue formation are crucial for regeneration of the osteochondral (OC) unit. Despite the tremendous effort in the field there is still no widely accepted system that stands out in terms of superior OC regeneration. Herein, we developed bilayered hydrogel composites (BHC) combining two structurally stratified layers fabricated from naturally derived and synthetic polymers, gellan-gum (GG) and hydroxyapatite (HAp), respectively. Two formulations were made from either low acyl GG (LAGG) alone or in combination with high acyl GG (HAGG) for the cartilage-like layer. Four bone-like layers were made of LAGG incorporating different ratios of hydroxyapatite (HAp). BHC were assembled in one single construct resulting in eight distinct bilayered constructs. Architectural observations by stereomicroscope and micro-CT (μ-CT) demonstrated a connected stratified structure with good ceramic dispersion within the bone-like layer. Swelling and degradation tests as well mechanical analyse showed a stable viscoelastic construct under dynamic forces. In-vitro studies by encapsulating rabbit's chondrocytes and osteoblasts in the respective layers showed the cytocompatibility of the BHC. Further studies comprising subcutaneous implantation in mice displayed a weak immune response after four weeks. OC orthotopic defects in the rabbit's knee were created and injected with the acellular BHC. OC tissue was regenerated four weeks after implantation as confirmed by cartilaginous and bony tissue formation assessed by histologic staining and μ-CT analysis. The successful fabrication of injectable BHC and their in-vitro and in-vivo performance may be seen as advanced engineered platforms to treat the challenging OC defects.
This material is based on works supported by the Portuguese Foundation for Science and Technology (FCT) under the OsteoCart project (PTDC/CTM-BPC/115977/2009) and for the M-ERA-NET/0001/2014 project. The authors are grateful to Teresa Oliveira for the assistance with histological studies. DR Pereira acknowledges the FCT for the individual grant (SFRH/BD/81356/2011) and JM Oliveira also thanks the FCT for the funds provided under the program Investigator FTC 2012 and 2015 (IF/00423/2012 and IF/01285/2015).
info:eu-repo/semantics/publishedVersion

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73f0e9b59ca13ed8c91efa512e51a5d0Test
https://hdl.handle.net/1822/56299Test

المؤلفون: Sofia, Amadori, Paola, Torricelli, Silvia, Panzavolta, Annapaola, Parrilli, Milena, Fini, Adriana, Bigi

المساهمون: Amadori, S., Torricelli, P., Panzavolta, S., Parrilli, A., Fini, M., Bigi, A.

مصطلحات موضوعية: Tissue Engineering, Tissue Scaffolds, multi-layered scaffold, osteochondral regeneration, hydroxyapatite, Mesenchymal Stem Cells, co-culture, Coculture Techniques, gelatin, Cartilage, Durapatite, Osteogenesis, Humans, Nanoparticles

الوصف: A promising strategy for osteochondral interface regeneration consists in the development of hybrid scaffolds, composed of distinct but integrated layers able to mimic the different regions of cartilage and bone. We developed multi-layered scaffolds by assembling a gelatin layer with layers containing different amounts of gelatin and hydroxyapatite nanocrystals, and using a gelatin solution (as a glue) to stick layers together. The scaffolds exhibit a high, interconnected porosity and mechanical properties varying with composition along the thickness of the scaffolds up to values of compressive stress and modulus of about 1 and 14 MPa, respectively. In vitro tests demonstrate that the different layers of the scaffolds promote chondrogenic and osteogenic differentiation of Human Mesenchimal Stem Cells (hMSC).

وصف الملف: STAMPA

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::eb3baf1371819e2813b8e69c9dde94a2Test
http://hdl.handle.net/11585/520001Test