التفاصيل البيبلوغرافية
| العنوان: |
Degradation of ZAP-70 following antigenic stimulation in human T lymphocytes: role of calpain proteolytic pathway. |
| المؤلفون: |
Penna, D., Müller, S., Martinon, F., Demotz, S., Iwashima, M., Valitutti, S. |
| المصدر: |
Journal of Immunology, vol. 163, no. 1, pp. 50-56 |
| سنة النشر: |
1999 |
| المجموعة: |
Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| مصطلحات موضوعية: |
Antigen-Presenting Cells/immunology, Antigen-Presenting Cells/metabolism, Antigens/immunology, Calpain/metabolism, Calpain/physiology, Cell Line, Transformed, Clone Cells, Down-Regulation/immunology, Humans, Hydrolysis, Lymphocyte Activation/drug effects, Membrane Proteins/antagonists & inhibitors, Membrane Proteins/metabolism, Protein-Tyrosine Kinases/antagonists & inhibitors, Protein-Tyrosine Kinases/metabolism, Receptors, Antigen, T-Cell/antagonists & inhibitors, T-Cell/metabolism, T-Lymphocytes/enzymology, T-Lymphocytes/immunology, Tetradecanoylphorbol Acetate/pharmacology, ZAP-70 Protein-Tyrosine Kinase |
| الوصف: |
T cell activation by the specific Ag results in dramatic changes of the T cell phenotype that include a rapid and profound down-regulation and degradation of triggered TCRs. In this work, we investigated the fate of the TCR-associated ZAP-70 kinase in Ag-stimulated T cells. T cells stimulated by peptide-pulsed APCs undergo an Ag dose-dependent decrease of the total cellular content of ZAP-70, as detected by FACS analysis and confocal microscopy on fixed and permeabilized T cell-APC conjugates and by Western blot on total cell lysates. The time course of ZAP-70 consumption overlaps with that of zeta-chain degradation, indicating that ZAP-70 is degraded in parallel with TCR internalization and degradation. Pharmacological activation of protein kinase C (PKC) does not induce ZAP-70 degradation, which, on the contrary, requires activation of protein tyrosine kinases. Two lines of evidence indicate that the Ca2+-dependent cysteine protease calpain plays a major role in initiating ZAP-70 degradation: 1) treatment of T cells with cell-permeating inhibitors of calpain markedly reduces ZAP-70 degradation; 2) ZAP-70 is cleaved in vitro by calpain. Our results show that, in the course of T cell-APC cognate interaction, ZAP-70 is rapidly degraded via a calpain-dependent mechanism. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| تدمد: |
0022-1767 |
| العلاقة: |
info:eu-repo/semantics/altIdentifier/pmid/10384098; info:eu-repo/semantics/altIdentifier/pissn/0022-1767; https://serval.unil.ch/notice/serval:BIB_65FC75322886Test; urn:issn:0022-1767 |
| الإتاحة: |
https://serval.unil.ch/notice/serval:BIB_65FC75322886Test |
| رقم الانضمام: |
edsbas.D3300FB9 |
| قاعدة البيانات: |
BASE |
