المؤلفون: Lam, Jesse H, Hill, Brian, Quang, Timothy, Amelard, Robert, Kim, Sehwan, Yazdi, Hossein S, Warren, Robert V, Cutler, Kyle B, Tromberg, Bruce J

المصدر: Journal of Biomedical Optics. 26(8)

مصطلحات موضوعية: Engineering, Physical Sciences, Biomedical Engineering, Bioengineering, Biomedical Imaging, Hemodynamics, Humans, Optical Imaging, Phantoms, Imaging, Spectroscopy, Near-Infrared, diffuse optical spectroscopy, imaging, chromophore, near-infrared, tissue metabolism, Optical Physics, Opthalmology and Optometry, Optics, Ophthalmology and optometry, Biomedical engineering, Atomic, molecular and optical physics

الوصف: Diffuse optical spectroscopic imaging (DOSI) is a versatile technology sensitive to changes in tissue composition and hemodynamics and has been used for a wide variety of clinical applications. Specific applications have prompted the development of versions of the DOSI technology to fit specific clinical needs. This work describes the development and characterization of a multi-modal DOSI (MM-DOSI) system that can acquire metabolic, compositional, and pulsatile information at multiple penetration depths in a single hardware platform. Additionally, a 3D tracking system is integrated with MM-DOSI, which enables registration of the acquired data to the physical imaging area. We demonstrate imaging, layered compositional analysis, and metabolism tracking capabilities using a single MM-DOSI system on optical phantoms as well as in vivo human tissue. We characterize system performance with a silicone phantom containing an embedded object. To demonstrate multi-layer sensitivity, we imaged human calf tissue with a 4.8-mm skin-adipose thickness. Human thenar tissue was also measured using a combined broadband DOSI and continuous-wave near-infrared spectroscopy method (∼15  Hz acquisition rate). High-resolution optical property maps of absorption (μa) and reduced scattering (μs  '  ) were recovered on the phantom by capturing over 1000 measurement points in under 5 minutes. On human calf tissue, we show two probing depth layers have significantly different (p

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/2dv4n9fvTest

المؤلفون: Yazdi, Hossein S, O’Sullivan, Thomas D, Leproux, Anais, Hill, Brian, Durkin, Amanda, Telep, Seraphim, Lam, Jesse, Yazdi, Siavash S, Police, Alice M, Carroll, Robert M, Combs, Freddie J, Strömberg, Tomas, Yodh, Arjun G, Tromberg, Bruce J

المصدر: Journal of Biomedical Optics. 22(4)

مصطلحات موضوعية: Physical Sciences, Biomedical and Clinical Sciences, Oncology and Carcinogenesis, Cancer, Biomedical Imaging, Adult, Carcinoma, Ductal, Breast, Diffusion, Female, Hemoglobins, Humans, Lipids, Models, Theoretical, Neoadjuvant Therapy, Oxyhemoglobins, Phantoms, Imaging, Spectrophotometry, Spectroscopy, Near-Infrared, Tomography, Optical, noninvasive, breast cancer, diffuse optical spectroscopy, oxygen metabolism, blood flow, diffuse correlation spectroscopy, Optical Physics, Biomedical Engineering, Opthalmology and Optometry, Optics, Ophthalmology and optometry, Biomedical engineering, Atomic, molecular and optical physics

الوصف: Diffuse optical spectroscopic imaging (DOSI) and diffuse correlation spectroscopy (DCS) are model-based near-infrared (NIR) methods that measure tissue optical properties (broadband absorption, ? a , and reduced scattering, ? s ? ) and blood flow (blood flow index, BFI), respectively. DOSI-derived ? a values are used to determine composition by calculating the tissue concentration of oxy- and deoxyhemoglobin ( HbO 2 , HbR), water, and lipid. We developed and evaluated a combined, coregistered DOSI/DCS handheld probe for mapping and imaging these parameters. We show that uncertainties of 0.3 ?? mm ? 1 (37%) in ? s ? and 0.003 ?? mm ? 1 (33%) in ? a lead to ? 53 % and 9% errors in BFI, respectively. DOSI/DCS imaging of a solid tissue-simulating flow phantom and

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/9jp023k2Test

المؤلفون: Manoochehr Karami, Yazdi-Ravandi, S., Ghaleiha, A., Olfatifar, M.

المصدر: Journal of Research in Health Sciences
Scopus-Elsevier

مصطلحات موضوعية: Male, Rural Population, Spatial Analysis, Epidemiology, Short Communication, Injury, Pilot Projects, Suicide, Attempted, Iran, Hospitals, Suicide, Logistic Models, Literacy, Odds Ratio, Educational Status, Humans, Female, Registries, Cities

الوصف: Background: Suicide behaviors are complex and multifactorial problems that in the most of the societies are considered as the public health challenge. However, its underlying reasons and spatial pattern remain unclear in Hamadan Province, western Iran. Study design: Secondary analysis of existing data. Methods: We assessed the spatial pattern pre-city regarding some influencing factors by scan-statistics and logistic regression to detect clusters areas and its comparison with other areas for the period of 2016- 2017. All of the registered cases of attempted suicide in a quality registry system of suicide in Sina (Farshchian) Hospital affiliated to Hamadan University of Medical Sciences, Hamadan, Iran were enrolled. Results: Two significant clusters were detected in study areas, formed with relative risk at 5.28 (P=0.001) and 6.33 (P=0.017), and with the centrality of Asadabad and Razan, respectively. Clusters and nonclusters areas were differed in terms of location (OR=0.15, 95%, CI: 0.07, 0.31), self-harms methods (OR=0.28, 95%, CI: 0.9, 0.88) and education. Residents of rural areas, illiterate people and non-drug user cases have more likely to be in a cluster. Conclusions: Clusters were not formed equally among cities of Hamadan Province. Accordingly, we suggest the implementation of appropriate, long-term and evidence-based educations for high-risk and vulnerable groups through the intersectoral interventions in different parts of Hamadan Province (considering the cluster and non-clusters areas) to avert deaths and related injuries from attempted suicide.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::97912025d83bb9dc833d070fc44eacd2Test
http://europepmc.org/articles/PMC6941641Test

المؤلفون: Hartmann, Hanna, Eltzschig, Holger K, Wurz, Helena, Hantke, Klaus, Rakin, Alexander, Yazdi, Amir S, Matteoli, Gianluca, Bohn, Erwin, Autenrieth, Ingo B, Karhausen, Jörn, Neumann, Diana, Colgan, Sean P, Kempf, Volkhard A J

مصطلحات موضوعية: Animals, Caco-2 Cells, Cell Hypoxia, Disease Models, Animal, Endothelial Cells, Enterobacter aerogenes, Enterobacteriaceae, Epithelial Cells, Female, Gene Expression Regulation, Hela Cells, Humans, Hydroxamic Acids, Hydroxylation, Hypoxia-Inducible Factor 1, alpha Subunit, Mice, Inbred BALB C, Inbred C57BL, Knockout, Oxygen, Peyer's Patches, Phenols, Procollagen-Proline Dioxygenase, Salmonella enterica, Siderophores, Thiazoles, Time Factors, Transcriptional Activation

الوصف: BACKGROUND & AIMS: Hypoxia inducible factor-1 (HIF-1) is the key transcriptional regulator during adaptation to hypoxia. Recent studies provide evidence for HIF-1 activation during bacterial infections. However, molecular details of how bacteria activate HIF-1 remain unclear. Here, we pursued the role of bacterial siderophores in HIF-1 activation during infection with Enterobacteriaceae. METHODS: In vivo, HIF-1 activation and HIF-1-dependent gene induction in Peyer's patches were analyzed after orogastric infection with Yersinia enterocolitica. The course of an orogastric Y enterocolitica infection was determined using mice with a deletion of HIF-1alpha in the intestine. In vitro, the mechanism of HIF-1 activation was analyzed in infections with Y enterocolitica, Salmonella enterica subsp enterica, and Enterobacter aerogenes. RESULTS: Infection of mice with Y enterocolitica led to functional activation of HIF-1 in Peyer's patches. Because mice with deletion of HIF-1alpha in the intestinal epithelium showed a significantly higher susceptibility to orogastric Y enterocolitica infections, bacterial HIF-1 activation appears to represent a host defense mechanism. Additional studies with Y enterocolitica, S enterica subsp enterica, or E aerogenes, and, moreover, application of their siderophores (yersiniabactin, salmochelin, aerobactin) caused a robust, dose-dependent HIF-1 response in human epithelia and endothelia, independent of cellular hypoxia. HIF-1 activation occurs most likely because of inhibition of prolylhydroxylase activity and is abolished upon infection with siderophore uptake deficient bacteria. CONCLUSIONS: Taken together, this study reveals what we believe to be a previously unrecognized role of bacterial siderophores for hypoxia-independent activation of HIF-1 during infection with human pathogenic bacteria. ; status: published

العلاقة: Gastroenterology vol:134 issue:3 pages:756-67; https://lirias.kuleuven.be/handle/123456789/280248Test; http://linkinghub.elsevier.com/retrieve/pii/S0016-5085Test(07)02169-5

الإتاحة: https://lirias.kuleuven.be/handle/123456789/280248Test
http://linkinghub.elsevier.com/retrieve/pii/S0016-5085Test(07)02169-5