المؤلفون: Jun, Cao, Liping, Jiang, Chengyan, Geng, Xiaofeng, Yao

المصدر: Wei sheng yan jiu = Journal of hygiene research. 38(4)

مصطلحات موضوعية: Acrylamide, Curcumin, Humans, Comet Assay, Free Radical Scavengers, Hep G2 Cells, Reactive Oxygen Species, DNA Damage

الوصف: To investigate the protective effects of curcumin on acrylamide (AA)-induced DNA damage in HepG2 cells.After pretreatment with curcumin (0.63, 1.25 and 2.50 microg/ml) for 1 hour, HepG2 cells were exposed to various concentrations of AA (0, 10, 20 mmol/L) for 1 h, comet assay was performed to determine DNA damage. The production of reactive oxygen species (ROS) was measured using the 2, 7-dichlorofluorescein diacetate (DCFH-DA) method.One-hour exposure of HepG2 cells to AA led to a dose-dependent increase in DNA fragmentation. When the cells were pretreated with curcumin for 1 h, the comet tail moment values were decreased compared to only AA-treated cells, and curcumin of 2.50 microg/nml significantly decreased the comet tail moment values compared to only AA-treated cells (P0.05). The DCF fluorescence intensity increased when the HepG2 cells were treated with 10 and 20 mmol/L AA (P0.01). When the cells were pretreated with curcumin, the level of ROS was significantly decreased compared to only AA-treated (P0.01).These data suggest that curcumin could attenuate AA-induced DNA damage in HepG2 cells. The protection is probably mediated by antioxidant protective mechanism.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::1535e50ffa19c36956a295cdc2239ea1Test
https://pubmed.ncbi.nlm.nih.gov/19689063Test

المؤلفون: Liping Jiang, Bao-Na Jiang, Chengyan Geng, Jun Cao, Ying Kong, Laifu Zhong, Li Jia, Yong Liu, Xiaofeng Yao

المصدر: Journal of agricultural and food chemistry. 56(24)

مصطلحات موضوعية: Antioxidant, Curcumin, Cell Survival, medicine.medical_treatment, DNA Fragmentation, medicine.disease_cause, chemistry.chemical_compound, Curcuma, Cell Line, Tumor, medicine, Humans, Cytotoxicity, Carcinogen, chemistry.chemical_classification, Reactive oxygen species, Acrylamide, Plant Extracts, General Chemistry, Free Radical Scavengers, chemistry, Biochemistry, Micronucleus test, Comet Assay, General Agricultural and Biological Sciences, Reactive Oxygen Species, Genotoxicity, DNA Damage

الوصف: Acrylamide (AA), a proven rodent carcinogen, has recently been discovered in foods heated at high temperatures. This finding raises public health concerns. In our previous study, we found that AA caused DNA fragments and increase of reactive oxygen species (ROS) formation and induced genotoxicity and weak cytotoxicity in HepG2 cells. Presently, curcumin, a natural antioxidant compound present in turmeric was evaluated for its protective effects. The results showed that curcumin at the concentration of 2.5 microg/mL significantly reduced AA-induced ROS production, DNA fragments, micronuclei formation, and cytotoxicity in HepG2 cells. The effect of PEG-catalase on protecting against AA-induced cytotoxicity suggests that AA-induced cytotoxicity is directly dependent on hydrogen peroxide production. These data suggest that curcumin could attenuate the cytotoxicity and genotoxicity induced by AA in HepG2 cells. The protection is probably mediated by an antioxidant protective mechanism. Consumption of curcumin may be a plausible way to prevent AA-mediated genotoxicity.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aced8e4fb18a3e7e0187ea1a9deaec9eTest
https://pubmed.ncbi.nlm.nih.gov/19530719Test