المؤلفون: Yun Hao Xu, Yuanpeng Li, Ying Jing, Balasubramanian Srinivasan, Jian-Ping Wang, Chengguo Xing, Xiaofeng Yao

المصدر: Angewandte Chemie. 121:2802-2805

مصطلحات موضوعية: Physics, Interleukin-6, Iron, Protein Array Analysis, Biotin, Metal Nanoparticles, Reproducibility of Results, Nanotechnology, Enzyme-Linked Immunosorbent Assay, General Chemistry, Biosensing Techniques, Cobalt, General Medicine, Catalysis, Magnetics, Microscopy, Electron, Transmission, Magnetic nanoparticles, Humans, Streptavidin

الوصف: Ein zeptomolarer Detektor: Ein hoch empfindlicher Chip mit Riesenmagnetwiderstand in Kombination mit FeCo-Nanopartikeln ermoglicht die lineare Detektion von nur 600–4500 Streptavidin-Molekulen. Schon unter nichtoptimierten Bedingungen erkennt das System uberdies humanes IL-6 mit einer 13-mal hoheren Empfindlichkeit als ELISA-Standardtechniken.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ce432f22aeaf008f628da5414637ac3Test
https://doi.org/10.1002/ange.200806266Test

المؤلفون: Chengyan Geng, Jun Cao, Xiang Xin Xue, Xiaomei Zhang, Liping Jiang, Xiaofeng Yao, Laifu Zhong

المصدر: Journal of Trace Elements in Medicine and Biology. 22:189-195

مصطلحات موضوعية: Lipopolysaccharides, Antioxidant, Lipopolysaccharide, medicine.medical_treatment, Inflammation, Pharmacology, medicine.disease_cause, Biochemistry, Monocytes, Cell Line, Inorganic Chemistry, chemistry.chemical_compound, Boric Acids, medicine, Humans, Secretion, RNA, Messenger, Sulfhydryl Compounds, Buthionine Sulfoximine, Tumor Necrosis Factor-alpha, Chemistry, Glutathione, Acetylcysteine, Gene Expression Regulation, Molecular Medicine, Tumor necrosis factor alpha, medicine.symptom, Oxidative stress, Intracellular

الوصف: Oxidative stress plays an important role during inflammatory diseases and antioxidant administration to diminish oxidative stress may arrest inflammatory processes. Boron has been implicated to modulate certain inflammatory mediators and regulate inflammatory processes. Here we investigated the role of the tripeptide glutathione (GSH) in modulating the effects of boric acid (BA) on lipopolysaccharide (LPS)-induced tumor necrosis factor alpha (TNF-alpha) formation in THP-1 monocytes. Interestingly, we found that BA had no significant effects on both TNF-alpha production and intracellular GSH contents, whereas it could inhibit LPS-induced TNF-alpha formation and ameliorated the d,l-buthionine-S,R-sulfoximine (BSO)-induced GSH depletion. Twenty-four hour incubation with BSO induced a decrease of the intracellular GSH and an increase of TNF-alpha. Treatment with N-acetyl-l-cysteine (NAC) did not significantly increase intracellular content of GSH but significantly reduced the secretion of TNF-alpha. BSO-pretreatment for 24h enhanced the LPS-induced secretion and mRNA expression of TNF-alpha further. BA inhibited LPS-stimulated TNF-alpha formation was also seen after GSH depletion by BSO. These results indicate that BA may have anti-inflammatory effect in the LPS-stimulated inflammation and the effect of BA on TNF-alpha secretion may be induced via a thiol-dependent mechanism.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b312266ad9b1957250a299e7c1b4a9e9Test
https://doi.org/10.1016/j.jtemb.2008.03.005Test

المؤلفون: Guang Yang, Qiujuan Li, Xiaofeng Yao, Liping Jiang, Xiaofang Liu, Chengyan Geng, Yuexia Wang, Min Chen, Ya-Nan Liu, Xiance Sun

المصدر: Toxins
Volume 7
Issue 8
Pages 3030-3044
Toxins, Vol 7, Iss 8, Pp 3030-3044 (2015)

مصطلحات موضوعية: Autophagosome, autophagy, Health, Toxicology and Mutagenesis, ATG5, lcsh:Medicine, Cathepsin D, Caspase 3, Biology, Toxicology, Permeability, Article, chemistry.chemical_compound, Humans, lysosomal membrane permeabilization, human liver HepG2 cells, Membrane potential, Membrane Potential, Mitochondrial, lcsh:R, Autophagy, apoptosis, Aurovertins, Hep G2 Cells, Intracellular Membranes, citreoviridin, Mycotoxins, Cell biology, chemistry, Apoptosis, Lysosomes, Pepstatin

الوصف: Citreoviridin (CIT) is a mycotoxin derived from fungal species in moldy cereals. In our previous study, we reported that CIT stimulated autophagosome formation in human liver HepG2 cells. Here, we aimed to explore the relationship of autophagy with lysosomal membrane permeabilization and apoptosis in CIT-treated cells. Our data showed that CIT increased the expression of LC3-II, an autophagosome biomarker, from the early stage of treatment (6 h). After treatment with CIT for 12 h, lysosomal membrane permeabilization occurred, followed by the release of cathepsin D in HepG2 cells. Inhibition of autophagosome formation with siRNA against Atg5 attenuated CIT-induced lysosomal membrane permeabilization. In addition, CIT induced collapse of mitochondrial transmembrane potential as assessed by JC-1 staining. Furthermore, caspase-3 activity assay showed that CIT induced apoptosis in HepG2 cells. Inhibition of autophagosome formation attenuated CIT-induced apoptosis, indicating that CIT-induced apoptosis was autophagy-dependent. Cathepsin D inhibitor, pepstatin A, relieved CIT-induced apoptosis as well, suggesting the involvement of the lysosomal-mitochondrial axis in CIT-induced apoptosis. Taken together, our data demonstrated that CIT induced autophagy-dependent apoptosis through the lysosomal-mitochondrial axis in HepG2 cells. The study thus provides essential mechanistic insight, and suggests clues for the effective management and treatment of CIT-related diseases.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07bf9e527059a36137a2716dbd9e1e14Test
https://pubmed.ncbi.nlm.nih.gov/26258792Test

المؤلفون: Xiance Sun, Xiaofeng Yao, Min Chen, Chengyan Geng, Yuexia Wang, Qiujuan Li, Ya-Nan Liu, Xiaofang Liu, Guang Yang, Liping Jiang

المصدر: Toxicon : official journal of the International Society on Toxinology. 95

مصطلحات موضوعية: Autophagosome, Programmed cell death, Cell Survival, ATG5, Biology, Toxicology, Autophagy-Related Protein 5, Microscopy, Electron, Transmission, Phagosomes, Autophagy, Humans, Viability assay, Cytotoxicity, PI3K/AKT/mTOR pathway, chemistry.chemical_classification, Reactive oxygen species, Adenine, Aurovertins, Hep G2 Cells, Cell biology, chemistry, Liver, RNA Interference, Reactive Oxygen Species, Microtubule-Associated Proteins

الوصف: Citreoviridin (CIT) is one of toxic mycotoxins derived from fungal species in moldy cereals. Whether CIT exerts hepatotoxicity and the precise molecular mechanisms of CIT hepatotoxicity are not completely elucidated. In this study, the inhibitor of autophagosome formation, 3-methyladenine, protected the cells against CIT cytotoxicity, and the autophagy stimulator rapamycin further decreased the cell viability of CIT-treated HepG2 cells. Knockdown of Atg5 with Atg5 siRNA alleviated CIT-induced cell death. These finding suggested the hypothesis that autophagic cell death contributed to CIT-induced cytotoxicity in HepG2 cells. CIT increased the autophagosome number in HepG2 cells observed under a transmission electron microscope, and this effect was confirmed by the elevated LC3-II levels detected through Western blot. Reduction of P62 protein levels and the result of LC3 turnover assay indicated that the accumulation of autophagosomes in the CIT-treated HepG2 cells was due to increased formation rather than impaired degradation. The pretreatment of HepG2 cells with the ROS inhibitor NAC reduced autophagosome formation and reversed the CIT cytotoxicity, indicating that CIT-induced autophagic cell death was ROS-dependent. In summary, ROS-dependent autophagic cell death of HpeG2 cells described in this study may help to elucidate the underlying mechanism of CIT cytotoxicity.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48df83aeb4a4e1a1ae9a5db829fb7452Test
https://pubmed.ncbi.nlm.nih.gov/25553592Test

المؤلفون: Laifu Zhong, Xiaofeng Yao

المصدر: Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 587:38-44

مصطلحات موضوعية: Carcinoma, Hepatocellular, Health, Toxicology and Mutagenesis, Biology, medicine.disease_cause, Risk Assessment, chemistry.chemical_compound, Dichlorofluorescein, Tumor Cells, Cultured, Genetics, medicine, Humans, Carcinogen, chemistry.chemical_classification, Fluorocarbons, Reactive oxygen species, Micronucleus Tests, Mutagenicity Tests, Liver Neoplasms, Molecular biology, Comet assay, Oxidative Stress, chemistry, Cell culture, Micronucleus test, Perfluorooctanoic acid, Comet Assay, Caprylates, Reactive Oxygen Species, Oxidative stress, DNA Damage

الوصف: Perfluorooctanoic acid (C 8 HF 15 O 2 , PFOA) is widely used in various industrial fields for decades and it is environmentally bioaccumulative. PFOA is known as a potent hepatocarcinogen in rodents. But it is not yet clear whether it is also carcinogenic in humans, and the genotoxic effects of PFOA on human cells have not yet been examined. In this study, the genotoxic potential of PFOA was investigated in human hepatoma HepG2 cells in culture using single cell gel electrophoresis (SCGE) assay and micronucleus (MN) assay. In order to clarify the underlying mechanism(s) we measured the intracellular generation of reactive oxygen species (ROS) using dichlorofluorescein diacetate as a fluorochrome. The level of oxidative DNA damage was evaluated by immunocytochemical analysis of 8-hydroxydeoxyguanosine (8-OHdG) in PFOA-treated HepG2 cells. PFOA at 50–400 μM caused DNA strand breaks and at 100–400 μM MN in HepG2 cells both in a dose-dependent manner. Significantly increased levels of ROS and 8-OHdG were observed in these cells. We conclude that PFOA exerts genotoxic effects on HepG2 cells, probably through oxidative DNA damage induced by intracellular ROS.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54a5f447623b1af847f90f8c88fcf516Test
https://doi.org/10.1016/j.mrgentox.2005.07.010Test

المؤلفون: Chengyan Geng, Jun Cao, Xiance Sun, Guang Yang, Jian Kang, Liping Jiang, Chuan-Zhou Gao, Liming Xu, Xiaofeng Yao, Laifu Zhong, Yufang Ma

المصدر: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 67

مصطلحات موضوعية: Autophagosome, Programmed cell death, Biology, Toxicology, Permeability, chemistry.chemical_compound, Western blot, Lysosome, medicine, Autophagy, Humans, Viability assay, PI3K/AKT/mTOR pathway, Fluorocarbons, medicine.diagnostic_test, Acridine orange, General Medicine, Hep G2 Cells, Intracellular Membranes, Cell biology, medicine.anatomical_structure, Biochemistry, chemistry, Alkanesulfonic Acids, Lysosomes, Food Science

الوصف: Perfluorooctane sulfonate (PFOS) is an emerging persistent organic pollutant widely distributed in the environment, wildlife and human. In this study, as observed under the transmission electron microscope, PFOS increased autophagosome numbers in HepG2 cells, and it was confirmed by elevated LC3-II levels in Western blot analysis. PFOS increased P62 level and chloroquine failed to further increase the expression of LC3-II after PFOS treatment, indicating that the accumulation of autophagosome was due to impaired degradation rather than increased formation. With acridine orange staining, we found PFOS caused lysosomal membrane permeabilization (LMP). In this study, autophasome formation inhibitor 3-methyladenine protected cells against PFOS toxicity, autophagy stimulator rapamycin further decreased cell viability and increased LDH release, cathepsin inhibitor did not influence cell viability of PFOS-treated HepG2 cells significantly. These further supported the notion that autophagic cell death contributed to PFOS-induced hepatotoxicity. In summary, the data of the present study revealed that PFOS induced LMP and consequent blockage of autophagy flux, leading to an excessive accumulation of the autophagosomes and turning autophagy into a destructive process eventually. This finding will provide clues for effective prevention and treatment of PFOS-induced hepatic disease.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1670925a5dbbbc5104dcbb0ced875b12Test
https://pubmed.ncbi.nlm.nih.gov/24561269Test

المؤلفون: Fangfang Song, Lun Zhang, Kexin Chen, Fengju Song, Hong Zheng, Xiaofeng Yao, Xiaoling Zhu, Qiang Zhang

مصطلحات موضوعية: Oncology, Adult, Male, Proto-Oncogene Proteins B-raf, medicine.medical_specialty, China, endocrine system diseases, Adolescent, Alcohol Drinking, Endocrinology, Diabetes and Metabolism, Single-nucleotide polymorphism, Biology, Polymorphism, Single Nucleotide, Papillary thyroid cancer, Young Adult, Endocrinology, Asian People, Gene Frequency, Risk Factors, Internal medicine, Genotype, medicine, Humans, Genetic Predisposition to Disease, Thyroid Neoplasms, Allele, Age of Onset, Genotyping, Allele frequency, Thyroid cancer, Genetic Association Studies, Aged, Genetics, Carcinoma, Smoking, Case-control study, Thyroid Cancer and Nodules, Middle Aged, medicine.disease, Carcinoma, Papillary, Thyroid Cancer, Papillary, Case-Control Studies, Lymphatic Metastasis, Female

الوصف: Papillary thyroid cancer (PTC) is a common malignancy that frequently harbors a high prevalence of somatic mutations in the oncogenic BRAF gene. As a novel prognostic molecular marker, this gene has drawn much attention in recent years for its potential utility in the risk prognosis and management of PTC. However, the contribution of the germline variants in this gene to PTC remains unclear. The study herein was aimed to investigate the potential association between the inherited BRAF variants and PTC based on a case-control study.We selected four single-nucleotide polymorphisms (SNPs) and took a systematic step to interrogate whether these SNPs of BRAF are associated with PTC risk by genotyping these SNPs from 368 patients with PTC and 564 healthy controls.In comparison of cases and controls for the four SNPs, no differences were observed in the genotypic and allelic frequencies, nor was there evidence of an association between BRAF SNPs and overall risk of PTC. After stratification, however, we found a significantly increased risk of PTC attributed to the SNP variants rs17161747, rs1042179, and rs3748093 for those with a family history of cancer, for smokers, and for both those of age45 years and nondrinkers, respectively. Further, in the PTC cases, those carrying the rs3748093 variant seemed to be less susceptible to developing lymph node metastases, but more likely to suffer from PTC at an earlier age (45 years).These preliminary results may provide evidence for the involvement of the common genetic variants scattered throughout the BRAF oncogene in the prediction of PTC onset and progression. In the future, enlarging the number of samples and performing functional studies in this gene may help to validate whether the association truly exists.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::088f46872df8395b767417ccf1c815c9Test
https://europepmc.org/articles/PMC3539251Test/

المؤلفون: Jun Cao, Xiaofeng Yao, Chunpeng Gao, Laifu Zhong, Liping Jiang, Chengyan Geng

المصدر: Phytomedicine : international journal of phytotherapy and phytopharmacology. 20(8-9)

مصطلحات موضوعية: Antioxidant, Cell Survival, medicine.medical_treatment, Pharmaceutical Science, Pharmacology, Mitochondrion, medicine.disease_cause, DNA, Mitochondrial, Antioxidants, chemistry.chemical_compound, Polysaccharides, Drug Discovery, medicine, Humans, Cytotoxicity, Nootropic Agents, chemistry.chemical_classification, Medicine, East Asian Traditional, Membrane Potential, Mitochondrial, Reactive oxygen species, biology, Plant Extracts, Glutathione, Hep G2 Cells, biology.organism_classification, Mitochondria, Oxidative Stress, Complementary and alternative medicine, chemistry, Phellinus linteus, Biochemistry, Phellinus, Tacrine, Hepatocytes, Molecular Medicine, Medicine, Traditional, Reactive Oxygen Species, Oxidative stress, medicine.drug, DNA Damage

الوصف: Tacrine (THA) was the first drug licensed for the treatment of Alzheimer's disease. Unfortunately, reversible hepatotoxicity is evident in about 30% of patients and limits its clinical use. The intracellular mechanisms have not yet been elucidated. Phellinus linteus (PL) is a mushroom that has long been used as a folk medicine. In our previous study, we found that PL could decrease the reactive oxygen species (ROS) formation in HepG2 cells. Presently, protective effects of PL on tacrine-induced ROS formation and mitochondria dysfunction were evaluated. The results showed that PL significantly reduced tacrine-induced ROS production, disruption of ΔΨm, 8-OHdG formation in mitochondrial DNA, and cytotoxicity in HepG2 cells. These data suggest that PL could attenuate the cytotoxicity and mitochondria dysfunction induced by tacrine in HepG2 cells. The protection is probably mediated by an antioxidant protective mechanism. Consumption of PL may be a plausible way to prevent tacrine-induced hepatotoxicity.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c3fbec23076f081dc881694af86db14Test
https://pubmed.ncbi.nlm.nih.gov/23523257Test

المؤلفون: Qiang Zhang, Yansheng Wu, Xiaofeng Yao, Liuyang Zhang, Xuan Zhou, Lun Zhang

المصدر: Journal of cancer research and clinical oncology. 138(2)

مصطلحات موضوعية: Adult, Male, Cancer Research, medicine.medical_specialty, China, Multivariate analysis, Kaplan-Meier Estimate, Logistic regression, Gastroenterology, Group A, Group B, Young Adult, Tongue, Internal medicine, Medicine, Humans, Survival rate, Aged, Neoplasm Staging, Hematology, business.industry, Cancer, General Medicine, Middle Aged, medicine.disease, Prognosis, Tongue Neoplasms, Survival Rate, medicine.anatomical_structure, Logistic Models, Oncology, Lymphatic Metastasis, Multivariate Analysis, Female, Neoplasm Recurrence, Local, business

الوصف: The purpose of this study was to compare the prognosis of oral tongue cancer patients and base of tongue cancer patients. One hundred oral tongue cancer patients (group A) were matched with 50 base of tongue cancer patients (group B) for gender, age, T-stage, and tumor differentiation in this study. Survival rate was performed using the Kaplan–Meier analysis, and multivariate analysis was conducted using the Logistic regression model. There was difference in the survival rate between the two groups. Three- and 5-year OS (overall survival) of the two groups were 65.0, 51.0% for group A and 40.0, 28.0% for group B, respectively. For the two groups, 3- and 5-year DSS (disease-specific survival) were 61.0, 46.0% for group A and 38.0, 26.0% for group B, respectively. Multivariate analysis showed that recurrence (P = 0.019) and regional lymph node metastasis (P = 0.043) were significant between the group A and group B patients. The oral tongue cancer patients had a better prognosis than base of tongue cancer patients. The difference in prognosis between the oral tongue cancer and the base of tongue cancer patients in this study was closely associated with the recurrence and regional lymph node metastasis. We conclude that the individual treatment should be used for base of tongue cancer patients.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f830ca3366a70bd1ab4ccdf9e4b396acTest
https://pubmed.ncbi.nlm.nih.gov/22139349Test

المؤلفون: Yuanpeng Li, Chengguo Xing, Balasubramanian Srinivasan, Marie A. Hugger, Ying Jing, Jian-Ping Wang, Xiaofeng Yao

المصدر: Journal of the American Chemical Society. 132(12)

مصطلحات موضوعية: Medical device, Chromatography, Time Factors, Chemistry, Interleukin-6, Iron, Early detection, Metal Nanoparticles, Nanotechnology, Enzyme-Linked Immunosorbent Assay, General Chemistry, Biosensing Techniques, Cobalt, Magnetoresistive sensor, Biochemistry, Catalysis, Biomarker (cell), Magnetics, Colloid and Surface Chemistry, Chronic disease, Biomarkers, Tumor, Disease biomarker, Humans, Biomarkers

الوصف: A novel giant magnetoresistive sensor and uniform high-magnetic-moment FeCo nanoparticles (12.8 nm)-based detecting platform with minimized detecting distance was developed for rapid biomolecule quantification from body fluids. Such a system demonstrates specific, accurate, and quick detection and quantification of interleukin-6, a low-abundance protein and a potential cancer biomarker, directly in 4 muL of unprocessed human sera. This platform is expected to facilitate the identification and validation of disease biomarkers. It may eventually lead to a low-cost personal medical device for chronic disease early detection, diagnosis, and prognosis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb704949919962a05643be187b264bb2Test
https://pubmed.ncbi.nlm.nih.gov/20192199Test