المؤلفون: Ming-Ming Tsai, Chung-Ying Tsai, Chia-Siu Wang, Kwang-Huei Lin, Liang-Mou Kuo, Hsiang-Cheng Chi, Pei-Hsuan Lu, Kam-Fai Lee, Hsiang-Wei Huang, Yang-Hsiang Lin, Chung-Guei Huang

المصدر: European Journal of Cancer. 64:137-148

مصطلحات موضوعية: Adult, Male, 0301 basic medicine, Cancer Research, Metastasis, 03 medical and health sciences, 0302 clinical medicine, Carcinoembryonic antigen, Downregulation and upregulation, Stomach Neoplasms, microRNA, Biomarkers, Tumor, medicine, Humans, Clinical significance, Aged, Aged, 80 and over, biology, Reverse Transcriptase Polymerase Chain Reaction, business.industry, Cancer, Middle Aged, Prognosis, medicine.disease, Carcinoembryonic Antigen, MicroRNAs, Circulating MicroRNA, 030104 developmental biology, ROC Curve, Oncology, Area Under Curve, Case-Control Studies, 030220 oncology & carcinogenesis, Immunology, biology.protein, Cancer research, Biomarker (medicine), Female, business

الوصف: miR-196a and/or miR-196b, involved in cancer initiation and progression, are frequently upregulated in tumour tissues. However, the clinical significance of these microRNAs in gastric cancer (GC) remains to be clarified. In the current study, we investigated the potential utility of circulating miR-196a/b as novel biomarkers for early detection and/or metastatic prognosis of GC. The quantitative real time-polymerase chain reaction data revealed markedly higher pre-operative circulating miR-196a and miR-196b levels in GC patients than healthy controls. Receiver-operating characteristics curve analysis showed that circulating miR-196a, miR-196b and combined miR-196a and miR-196b (miR-196a/b) are more effective than carcinoembryonic antigen or carbohydrate antigen 19-9 alone in distinguishing GC patients from healthy controls, with higher sensitivity and specificity. Circulating miR-196a exhibited higher diagnostic capacity than combined miR-196a/b or miR-196b alone, highlighting its potential as an effective plasma biomarker for GC. In clinicopathological analysis, elevated circulating miR-196a/b levels were highly correlated with metastatic potential or more advanced stages of disease and poorer survival. In addition, the expression levels of circulating miR-196a/b were reduced after surgical resection in GC patients. Taken together, we propose that circulating miR-196a/b serve as a more sensitive and specific novel biomarker than carbohydrate antigen 19-9 for GC monitor, diagnosis and prognosis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61ce23d144338d3436ae490b50584bbbTest
https://doi.org/10.1016/j.ejca.2016.05.007Test

المؤلفون: Chung-Ying Tsai, Ming-Ming Tsai, Pei-Hsuan Lu, Kwang-Huei Lin, Hsiang-Wei Huang, Kam-Fai Lee, Chia-Siu Wang, Liang-Mou Kuo, Yang-Hsiang Lin, Hsiang-Cheng Chi

المصدر: Oncotarget

مصطلحات موضوعية: miR-26b, Adult, Male, alpha Karyopherins, 0301 basic medicine, Proto-Oncogene Proteins c-jun, Karyopherin alpha 2, KPNA2, c-jun, medicine.disease_cause, Metastasis, 03 medical and health sciences, 0302 clinical medicine, Bacterial Proteins, Cell Movement, Stomach Neoplasms, Cell Line, Tumor, microRNA, medicine, Humans, Neoplasm Metastasis, Promoter Regions, Genetic, 3' Untranslated Regions, Aged, Aged, 80 and over, Antigens, Bacterial, Helicobacter pylori, business.industry, Three prime untranslated region, gastric cancer, Cancer, Alpha Karyopherins, Middle Aged, Prognosis, medicine.disease, Gene Expression Regulation, Neoplastic, MicroRNAs, Treatment Outcome, 030104 developmental biology, Oncology, 030220 oncology & carcinogenesis, Immunology, Disease Progression, Cancer research, Female, Ectopic expression, business, Carcinogenesis, Research Paper

الوصف: // Ming-Ming Tsai 1, 3, * , Hsiang-Wei Huang 2, * , Chia-Siu Wang 3, * , Kam-Fai Lee 4 , Chung-Ying Tsai 2 , Pei-Hsuan Lu 5 , Hsiang-Cheng Chi 2 , Yang-Hsiang Lin 2 , Liang-Mou Kuo 3 , Kwang-Huei Lin 2, 6 1 Department of Nursing, Division of Basic Medical Sciences and Research Center for Industry of Human Ecology, Chang-Gung University of Science and Technology, Taoyuan, 333, Taiwan 2 Department of Biochemistry, College of Medicine, Chang-Gung University, Taoyuan, 333, Taiwan 3 Department of General Surgery, Chang Gung Memorial Hospital at Chia-yi, Chia-yi, 613, Taiwan 4 Department of Pathology, Chang Gung Memorial Hospital, Chia-yi, 613, Taiwan 5 Department of Dermatology, Chang Gung Memorial Hospital, Chang Gung University, Taipei, 10508, Taiwan 6 Liver Research Center, Chang Gung Memorial Hospital, Linkou, Taoyuan, 333, Taiwan * These authors contributed equally to this work Correspondence to: Kwang-Huei Lin, email: khlin@mail.cgu.edu.tw Keywords: miR-26b, KPNA2, c-jun, prognosis, gastric cancer Received: July 17, 2015 Accepted: March 12, 2016 Published: April 07, 2016 ABSTRACT MicroRNAs (miRNA) play an important role in carcinogenesis. Previously, we identified miR-26b as a significantly downregulated miRNA in gastric cancer (GC) tissues ( n = 106) based on differential quantitative RT-PCR (RT-qPCR) miRNA expression profiles. In the current study, we aimed to clarify the potential role of miR-26b and related target genes in GC progression. Downregulation of miR-26b was associated with advanced tumor-node-metastasis stage (TNM stage) and poor 5-year survival rate. Forced expression of miR-26b led to inhibition of GC cell migration and invasion in vitro and lung metastasis formation in vivo . Conversely, depletion of miR-26b had stimulatory effects. Additionally, miR-26b affected GC cell behavior through negative regulation of the metastasis promoter, karyopherin alpha 2 (KPNA2). Ectopic expression of miR-26b induced a reduction in KPNA2 protein levels, confirmed by luciferase assay data showing that miR-26b directly binds to the 3’ untranslated regions (UTR) of KPNA2 mRNA. Furthermore, miR-26b and KPNA2 mRNA/protein expression patterns were inversely correlated in GC tissues. Cag A of Helicobacter pylori ( Hp ) enhanced miR-26b levels through regulation of the KPNA2/c-jun pathway. Taken together, our data indicate that miR-26b plays an anti-metastatic role and is downregulated in GC tissues via the KPNA2/c-jun pathway. Based on the study findings, we propose that miR-26b overexpression or KPNA2/c-jun suppression may have therapeutic potential in inhibiting GC metastasis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4221856cd90c72d33b391382bf4b12b0Test
https://doi.org/10.18632/oncotarget.8629Test

المؤلفون: Chung-Ying Tsai, Wen-Yu Chuang, Hsiang-Cheng Chi, Chau-Ting Yeh, Yang-Hsiang Lin, Meng-Han Wu, Kwang-Huei Lin, Ya-Hui Huang, I-Hsiao Chung, Ching-Ying Chen, Chia-Jung Yu

المصدر: Endocrine-related cancer. 25(12)

مصطلحات موضوعية: 0301 basic medicine, Male, Cancer Research, Thyroid Hormones, Carcinoma, Hepatocellular, Carcinogenesis, Endocrinology, Diabetes and Metabolism, Cell, Mice, Nude, Biology, medicine.disease_cause, 03 medical and health sciences, Endocrinology, Downregulation and upregulation, Cell Line, Tumor, Cyclins, medicine, Animals, Humans, Receptor, Aged, Receptors, Thyroid Hormone, Cell growth, Cyclin-Dependent Kinase 2, Liver Neoplasms, Cancer, Middle Aged, medicine.disease, 030104 developmental biology, Cyclin E2, medicine.anatomical_structure, Oncology, Cancer research, Female, RNA, Long Noncoding, Hormone

الوصف: Thyroid hormone (T3) and its receptor (TR) are involved in cancer progression. While deregulation of long non-coding RNA (lncRNA) expression has been detected in many tumor types, the mechanisms underlying specific involvement of lncRNAs in tumorigenicity remain unclear. Experiments from the current study revealed negative regulation of BC200 expression by T3/TR. BC200 was highly expressed in hepatocellular carcinoma (HCC) and effective as an independent prognostic marker. BC200 promoted cell growth and tumor sphere formation, which was mediated via regulation of cell cycle-related genes and stemness markers. Moreover, BC200 protected cyclin E2 mRNA from degradation. Cell growth ability was repressed by T3, but partially enhanced upon BC200 overexpression. Mechanistically, BC200 directly interacted with cyclin E2 and promoted CDK2–cyclin E2 complex formation. Upregulation of cell cycle-related genes in hepatoma samples was positively correlated with BC200 expression. Our collective findings support the utility of a potential therapeutic strategy involving targeting of BC200 for the treatment of HCC.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97d3beb4328c70312714e7c3032bc9aeTest
https://pubmed.ncbi.nlm.nih.gov/30400024Test

المؤلفون: Chien-Fu Hung, Chen-Chun Lin, Ta-Sen Yeh, Kwang-Huei Lin, Wei-Chen Lee, Chau-Ting Yeh, Yu-De Chu, Ya-Hui Huang

المصدر: Asia-Pacific journal of clinical oncology. 14(5)

مصطلحات موضوعية: Sorafenib, Oncology, Male, medicine.medical_specialty, Thyroid Hormones, Carcinoma, Hepatocellular, medicine.medical_treatment, Thyroid Gland, Antineoplastic Agents, Thyroid Function Tests, Cohort Studies, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, medicine, Humans, Stage (cooking), Chemotherapy, business.industry, Liver Neoplasms, General Medicine, Middle Aged, medicine.disease, digestive system diseases, Survival Rate, Treatment Outcome, 030220 oncology & carcinogenesis, Hepatocellular carcinoma, Cohort, 030211 gastroenterology & hepatology, Female, Liver function, Thyroid function, Liver cancer, business, hormones, hormone substitutes, and hormone antagonists, Biomarkers, medicine.drug

الوصف: Aim A sustained proportion of advanced hepatocellular carcinoma (HCC) patients worldwide received either chemotherapy or sorafenib. However, to date, effective and convenient biomarkers to predict their therapeutic outcomes remained elusive. Hypothyroidism was associated with favorable anticancer treatment outcomes in several advanced cancers. Here, we aimed to investigate the potential of using thyroid-stimulating hormone (TSH) and free T4 (FT4) levels as biomarkers to predict clinical outcomes in HCC patients receiving chemotherapy or sorafenib. Methods Total 123 advanced HCC patients at Barcelona Clinical Liver Cancer Stage C were included. They were separated into two cohorts, one treated by sorafenib (n = 62) and the other by chemotherapy (n = 61). Clinical data including TSH and FT4 were retrieved and correlated with treatment outcomes. Results Because of restriction in local insurance policy, the baseline liver function reserve was better in patients receiving sorafenib. Therefore, the two cohorts were analyzed separately. The results showed that a higher (> median) TSH × FT4 value was independently associated with favorable time-to-tumor progression (P = 0.006) and overall survival (P = 0.002) if chemotherapy was provided; whereas it was associated with unfavorable time-to-tumor progression (P = 0.017) and overall survival (P = 0.001) if sorafenib was administrated. These opposite associations remained valid when patients with Child-Pugh class A liver function from either cohort were included for analysis. Conclusion A novel thyroid function index, TSH × FT4, significantly predicted opposite clinical outcomes in advanced HCC patients receiving sorafenib or chemotherapy treatment.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c63e35439db2f4c4872c437fef66d8ddTest
https://pubmed.ncbi.nlm.nih.gov/29785761Test

المؤلفون: Cheng Yi Chen, Yi Hsin Tseng, Hsiang Cheng Chi, Chia Siu Wang, Ming Ming Tsai, Wan-Li Cheng, Chung-Ying Tsai, Crystal D. Lin, Jun I. Wu, Lu-Hai Wang, Kwang-Huei Lin

المصدر: Clinical Cancer Research. 20:2276-2288

مصطلحات موضوعية: Adult, Male, Vascular Endothelial Growth Factor A, Oncology, Cancer Research, medicine.medical_specialty, MMP2, Gene Expression, Biology, Metastasis, Cell Movement, Stomach Neoplasms, Cell Line, Tumor, Internal medicine, medicine, Cluster Analysis, Humans, Neoplasm Invasiveness, Neoplasm Metastasis, beta Catenin, Aged, Neoplasm Staging, Tumor marker, Aged, 80 and over, Gene Expression Profiling, Interleukins, Interleukin-8, Cancer, Cell migration, Middle Aged, Prognosis, medicine.disease, Tumor Burden, Gene Expression Regulation, Neoplastic, Matrix Metalloproteinase 9, Tumor progression, Cancer cell, Disease Progression, Matrix Metalloproteinase 2, Female, Ectopic expression, Proto-Oncogene Proteins c-akt, Signal Transduction

الوصف: Purpose: The proinflammatory cytokine interleukin-32 (IL-32) is a novel tumor marker highly expressed in various human carcinomas, including gastric cancer. However, its effects on prognosis of patients with gastric cancer and cancer metastasis are virtually unknown at present. The main aim of this study was to explore the clinical significance of IL-32 in gastric cancer and further elucidate the molecular mechanisms underlying IL-32–mediated migration and invasion. Experimental Design: Gastric cancer cells with ectopic expression or silencing of IL-32 were examined to identify downstream molecules and establish their effects on cell motility, invasion, and lung metastasis in vivo. Results: IL-32 was significantly upregulated in gastric cancer and positively correlated with aggressiveness of cancer and poor prognosis. Ectopic expression of IL-32 induced elongated morphology and increased cell migration and invasion via induction of IL-8, VEGF, matrix metalloproteinase 2 (MMP2), and MMP9 expression via phosphor-AKT/phospho-glycogen synthase kinase 3β/active β-catenin as well as hypoxia-inducible factor 1α (HIF-1α) signaling pathways. Conversely, depletion of IL-32 in gastric cancer cells reversed these effects and decreased lung colonization in vivo. Examination of gene expression datasets in oncomine and staining of gastric cancer specimens demonstrated the clinical significance of IL-32 and its downstream molecules by providing information on their coexpression patterns. Conclusions: IL-32 contributes to gastric cancer progression by increasing the metastatic potential resulting from AKT, β-catenin, and HIF-1α activation. Our results clearly suggest that IL-32 is an important mediator for gastric cancer metastasis and independent prognostic predictor of gastric cancer. Clin Cancer Res; 20(9); 2276–88. ©2014 AACR.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1fd21b94a30a6a8afaebf47156c0b23Test
https://doi.org/10.1158/1078-0432.ccr-13-1221Test

المؤلفون: Ting-Chang Chang, Hung Hsueh Chou, Jung Erh Yang, Kwang-Huei Lin, Tzu I. Wu, Chyong-Huey Lai, Swei Hsueh, Chun-Sen Hsu, Chi Ju Yeh, Mei Shan Jao

المصدر: International Journal of Gynecological Pathology. 32:482-492

مصطلحات موضوعية: Adult, Oncology, medicine.medical_specialty, Mitotic index, Proliferative index, Sarcoma, Endometrial Stromal, Disease-Free Survival, Pathology and Forensic Medicine, Young Adult, Pregnancy, Internal medicine, Progesterone receptor, Biomarkers, Tumor, medicine, Humans, Stage (cooking), Survival rate, Aged, Neoplasm Staging, Retrospective Studies, Univariate analysis, Endometrial stromal sarcoma, business.industry, Obstetrics and Gynecology, Middle Aged, Prognosis, medicine.disease, Endometrial Neoplasms, Survival Rate, Ki-67 Antigen, Receptors, Estrogen, Female, Sarcoma, Receptors, Progesterone, business, Follow-Up Studies

الوصف: We aimed to investigate the clinicopathologic features, immunohistochemical studies, and prognosis in patients with endometrial stromal sarcoma (ESS). Clinical information was reviewed retrospectively for cases of ESS (1985-2009). A histologic review and immunohistochemical staining for the estrogen receptor, progesterone receptor, c-Kit, CD-10, Ki-67, and m-TOR were performed. Sixty-one patients (median age, 44 y; range, 22-71) were eligible for analysis (1988 International Federation of Gynecology and Obstetrics Stage I, 43; Stage II, 2; Stage III, 11; Sage IV, 4; unstaged, 1). The median follow-up period for survivors was 73 mo. Of those, the patients who underwent an adnexectomy and a pelvic lymphadenectomy, 15% and 13%, respectively, revealed metastasis. There were 20 relapses/persistence, including 13 (65%) in the pelvis and abdomen and 7 (35%) in distant sites. Eight patients died from ESS at a median duration of 14.5 mo (range, 2-50 mo) after relapse. Five- and 10-yr cancer-specific survival (CSS) rates were 88% and 85%, respectively; and 5- and 10-yr progression-free survival rates were 69% and 57%, respectively. Stage, residual disease, and high proliferative index of Ki-67 were significant prognostic factors for both progression-free survival and CSS in a univariate analysis, in addition to mitotic index for CSS. Multivariate analysis selected only residual disease as an independent variable for progression-free survival and stage and residual disease for CSS. Our results support using clinical Stage I, no residual disease, low proliferative index of Ki-67, and estrogen receptor/progesterone receptor overexpression as potential biomarkers to select patients with ESS for fertility-preservation surgery (5 such patients were alive and free).

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7500ef377051b404f2bb222bc43317b0Test
https://doi.org/10.1097/pgp.0b013e3182729131Test

المؤلفون: Jin-Yo Hu, Yang-Hsiang Lin, Kwang-Huei Lin, Tzu-I Wu, Chyong-Huey Lai, Chia-Jung Liao, I-Hsiao Chung, Pei-ju Tai

المصدر: Oncotarget

مصطلحات موضوعية: 0301 basic medicine, Adult, Pathology, medicine.medical_specialty, Epithelial-Mesenchymal Transition, cervical cancer, Uterine Cervical Neoplasms, Mice, SCID, Lipocalin, Metastasis, 03 medical and health sciences, Mice, 0302 clinical medicine, Lipocalin-2, medicine, Animals, Humans, metastasis, Neoplasm Invasiveness, Epithelial–mesenchymal transition, Aged, Cervical cancer, Gene knockdown, biology, business.industry, Middle Aged, medicine.disease, invasion, Up-Regulation, Fibronectin, 030104 developmental biology, Oncology, 030220 oncology & carcinogenesis, Cancer cell, biology.protein, Heterografts, Ectopic expression, Female, business, lipocalin2, Research Paper

الوصف: Cervical carcinoma is the third-most common cause of cancer-related deaths in women worldwide. However, the molecular mechanisms underlying the metastasis of cervical cancer are still unclear. Oligonucleotide microarrays coupled with bioinformatics analysis show that cytoskeletal remodeling and epithelial-to- mesenchymal transition (EMT) are significant pathways in clinical specimens of cervical cancer. In accord with clinical observations demonstrating ectopic expression of lipocalin 2 (LCN2), an oncogenic protein associated with EMT, in malignant tumors, was significantly upregulated in cervical cancer and correlated with lymph node metastasis. Overexpression of LCN2 enhanced tumor cell migration and invasion both in vitro and in vivo. Conversely, knockdown or neutralization of LCN2 reduced tumor cell migration and invasion. LCN2-induced migration was stimulated by activation of the EMT-associated proteins, Snail, Twist, N-cadherin, fibronectin, and MMP-9. Our findings collectively support a potential role of LCN2 in cancer cell invasion through the EMT pathway and suggest that LCN2 could be effectively utilized as a lymph node metastasis marker in cervical cancer.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7482b0ce1f5442c5c2acb0ef4e8715efTest
https://pubmed.ncbi.nlm.nih.gov/26840566Test

المؤلفون: Tzu I. Wu, Ting-Chang Chang, Ying Liang, Chia Siu Wang, Shih-Ming Jung, Kwang-Huei Lin, Chyong-Huey Lai, Chia Jung Liao, Ya-Hui Huang, Ming Ming Tsai

المصدر: Cancer Science. 102:2255-2263

مصطلحات موضوعية: Adult, Cancer Research, Pathology, medicine.medical_specialty, Lung Neoplasms, Transplantation, Heterologous, Cell, Protein Disulfide-Isomerases, Uterine Cervical Neoplasms, Kaplan-Meier Estimate, Adenocarcinoma, Biology, HeLa, Carcinoma, Adenosquamous, Mice, Cell Line, Tumor, Biomarkers, Tumor, medicine, Carcinoma, Animals, Humans, Neoplasm Invasiveness, Clinical significance, RNA, Small Interfering, Aged, Cervical cancer, General Medicine, Middle Aged, Prognosis, medicine.disease, biology.organism_classification, Survival Analysis, Transplantation, medicine.anatomical_structure, Oncology, Lymphatic Metastasis, Carcinoma, Squamous Cell, Cancer research, Immunohistochemistry, Female, RNA Interference, Neoplasm Transplantation, HeLa Cells

الوصف: Human papilloma virus infection is critical but not sufficient to cause cervical cancer. Molecular markers of cervical carcinogenesis are essential. The aim of this study was to identify aberrantly expressed proteins in cervical cancer and determine their clinical significance. A two-dimensional polyacrylamide gel electrophoresis (2-DE) proteomic strategy was used for screening candidate proteins. Immunoblotting and immunohistochemical (IHC) analyses were performed to confirm the results of 2-DE, and the clinical significance was estimated. Glucose-regulated protein 58 (Grp58) was overexpressed in 73% of cancers. The IHC staining showed that the Grp58 histoscore was significantly higher in patients with adenocarcinoma (AD) compared with squamous cell carcinoma (P < 0.05). Grp58 staining was intense in AD with a penetration depth greater than half of the cervical stroma (P = 0.033). High Grp58 expression was associated with low overall survival and recurrence-free survival (RFS) rates (P = 0.007 and P = 0.013, respectively). In multivariate analysis, high Grp58 expression (P = 0.042) and lymph node metastasis (P = 0.026) were determined as independent prognostic factors for RFS. Patients exhibiting both high Grp58 expression and lymph node metastasis displayed poorer outcomes than the other patient groups. In functional studies, knockdown of Grp58 in HeLa cells led to decreased cell invasiveness and inhibition of lung metastasis in a xenograft mouse model. In conclusion, Grp58 serves as a potent prognostic factor of cervical AD. Estimation of the Grp58 index in conjunction with the lymph node metastasis status might aid in predicting the prognosis of cervical AD.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8eeeb0e2aa19e652a42c9f6c345c9082Test
https://doi.org/10.1111/j.1349-7006.2011.02102.xTest

المؤلفون: Ming Hung Tsai, Chia Siu Wang, Chia Jung Liao, Kwang-Huei Lin, Ya-Hui Huang, Ming Ming Tsai, Tzu I. Wu, Chung Yuan Hsu, Chyong-Huey Lai, Ting-Chang Chang

المصدر: Gynecologic Oncology. 120:135-144

مصطلحات موضوعية: Adult, Cyclin-Dependent Kinase Inhibitor p21, Pathology, medicine.medical_specialty, Epithelial-Mesenchymal Transition, Uterine Cervical Neoplasms, Cell Growth Processes, macromolecular substances, medicine.disease_cause, Cell Movement, Biomarkers, Tumor, medicine, Humans, Vimentin, Cyclin D1, Epithelial–mesenchymal transition, Survival rate, Gelsolin, Aged, Neoplasm Staging, Retrospective Studies, Tumor marker, Cervical cancer, business.industry, Gene Expression Profiling, Obstetrics and Gynecology, Middle Aged, Cadherins, Prognosis, medicine.disease, Fibronectins, Up-Regulation, Survival Rate, Oncology, Case-Control Studies, Gene Knockdown Techniques, Cancer cell, Cancer research, Matrix Metalloproteinase 2, Immunohistochemistry, Female, Carcinogenesis, business, HeLa Cells

الوصف: Cervical carcinoma is the second most common cause of death from gynecological cancers worldwide. Knowledge of the molecular mechanisms underlying the tumorigenesis of cervical cancer cell, except human papilloma virus infection, is limited.A microarray was used to study the differential expression of genes in cancerous tissues to identify new molecular markers for diagnosis and prognosis. Their differential expression was confirmed with Western blotting and immunohistochemical analyses. The clinical correlations and prognostic significance of the aberrantly expressed proteins were evaluated to identify novel biomarkers of cervical cancer.The expression of gelsolin was significantly upregulated in 78% of patients with cervical cancer, and gelsolin was selected for further study. Gelsolin expression was stronger in cervical tumor tissues than in the surrounding noncancerous tissues (P0.001). Gelsolin expression in the plasma of cervical cancer patients was increased 2.2-fold compared with that of healthy control subjects (P0.001). The levels of plasma gelsolin in the early and late stages were significantly different (P=0.006). According to immunohistochemical analysis, increased gelsolin expression was associated with histological type and FIGO stage II. The 5-year overall survival and recurrence-free survival rates for the low-expression group (cut-off=115) were significantly higher than those of the high-expression group. Cancer cells with reduced gelsolin expression exhibited reduced migration and proliferation.These results provide strong evidence that gelsolin plays an important role in cellular proliferation and migration in cervical cancer and suggest that gelsolin is a promising marker for cervical cancer screening and prognosis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6513e17f26cb7a25da77580ede308b29Test
https://doi.org/10.1016/j.ygyno.2010.10.005Test

المؤلفون: Pei-Jung Chung, Kwang-Huei Lin, Ming-Ming Tsai, I-Hsiao Chung, Chung-Ying Tsai, Cheng Yi Chen, Hsiang-Cheng Chi, Yi-Hsin Tseng, Chia-Siu Wang, Yang-Hsiang Lin, Ching-Ying Chen

المصدر: Cancer letters. 351(2)

مصطلحات موضوعية: Male, Cancer Research, Cell, Down-Regulation, In situ hybridization, Biology, medicine.disease_cause, Transfection, Metastasis, Radixin, Cell Movement, Stomach Neoplasms, Cell Line, Tumor, microRNA, medicine, Tumor Cells, Cultured, Humans, Genes, Tumor Suppressor, Neoplasm Metastasis, Aged, Neoplasm Staging, Cancer, Membrane Proteins, Middle Aged, medicine.disease, Prognosis, Molecular biology, Immunohistochemistry, Up-Regulation, Gene Expression Regulation, Neoplastic, Cytoskeletal Proteins, MicroRNAs, medicine.anatomical_structure, Oncology, Tumor progression, Cancer research, Female, Carcinogenesis

الوصف: MicroRNAs (miRNAs) play an important role to contribute carcinogenesis. The aim of the current study was to identify useful biomarkers from miRNAs. Differential miRNA profiles were analyzed using the miRNA qRT-PCR-based assay. Two of the most upregulated miRNAs were selected and validated. The miR-196a/-196b levels were significantly increased in gastric cancer (GC) tissues (n=109). Overexpression of miR-196a/-196b was significantly associated with tumor progression and poorer 5-year survival outcomes. Overexpression of miR-196a/-196b enhances GC cell migration and invasion. Further, radixin was identified as a target gene of miR-196a/-196b. Elevated miR-196a/-196b expression in GC cells led to reduced radixin protein levels and vice versa. Notably, an inverse correlation between miR-196a/-196b and radixin mRNA and protein expression was observed in GC tissues with in situ hybridization and immunohistochemistry analyses. Together, miR-196a/-196b inhibitory oligonucleotides or overexpression of the radixin may thus have therapeutic potential in suppressing GC metastasis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43e1a1846ca6663042f7c12148595e03Test
https://pubmed.ncbi.nlm.nih.gov/28137419Test