المؤلفون: Anju Mahendru-Singh, R. B. Shukla, Tej Pratap Jitendra Kumar, Namita Bedi, Rajeev Ranjan Kumar, Sumit K. Singh, Savita Saini, Arvind Kumar Ahlawat, Anjali Rai

المصدر: Journal of Plant Biochemistry and Biotechnology. 31:410-420

مصطلحات موضوعية: Cross breeding, food and beverages, Plant Science, Marker-assisted selection, Biology, Background selection, Rust, Horticulture, Backcrossing, Grain yield, Allele, Agronomy and Crop Science, Gene, Biotechnology

الوصف: The genes for grain softness and rust resistance were transferred from a soft grained Australian wheat variety (Barham) to a hard grained Indian wheat variety (DBW14) through marker assisted back cross breeding. Backcrosses were made with recurrent parent in the BC1F1 plants that were found positive for markers of Pina-D1a, Pina-D1b and Lr37b genes. The BC2F1 plants were further screened and those that possessed the PinaD1a and Lr37b alleles were advanced up to BC2F4 with continued marker assisted selection and field screening. 48 SSR markers that were heterozygous in the BC1F1 plants were used for background selection in three BC2F1 plants derived from three respective BC1F1s. Further generations were screened for morphological traits in the field and 16 BC2F4 lines were finally selected through combined field and marker screening. These 16 near isogenic lines (NILs) had lower grain hardness index (13.86–47.13) than the recurrent parent DBW 14 (81.76) and displayed more than 95% of the recurrent parent genome. 12 of the NILs had significantly higher grain yield than DBW 14. Combined foreground and background selection strategy coupled with field screening, was thus, found to be an effective strategy for transferring grain softness in wheat.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::7a23e72de892ba2346b91fa15b4c299eTest
https://doi.org/10.1007/s13562-021-00712-xTest

المؤلفون: Anju Mahendru-Singh, Nagenahalli Dharmegowda Rathan, Velu Govindan, Maria Itria Ibba

المصدر: Frontiers in Sustainable Food Systems, Vol 4 (2020)

مصطلحات موضوعية: wheat quality, Triticum aestivum (bread wheat), Biofortification, lcsh:TX341-641, Horticulture, Management, Monitoring, Policy and Law, grain Zn and Fe, Protein content, biofortification, Glutenin, Food science, Common wheat, chemistry.chemical_classification, Global and Planetary Change, biology, lcsh:TP368-456, Ecology, food and beverages, Micronutrient, Gluten, lcsh:Food processing and manufacture, chemistry, Food products, biology.protein, Grain yield, lcsh:Nutrition. Foods and food supply, Agronomy and Crop Science, glutenins composition, Food Science

الوصف: Development of biofortified wheat lines has emerged as a sustainable solution to alleviate malnutrition. However, for these varieties to be successful, it is important that they meet the minimum quality criteria required to produce the local food products. In the present study, a set of 94 biofortified common wheat lines were analyzed for their grain micronutrients content (Fe and Zn) and for their processing quality and glutenin profile. Most of the analyzed lines exhibited a grain Zn concentration greater than the non-biofortified check varieties, of at least 3 ppm. The content of both Fe and Zn appeared to be significantly associated with grain protein content (r = 0.21–0.65; p < 0.01) but not with grain yield or other wheat quality traits. Wide allelic variation was observed at both the high-molecular-weight glutenin (HMW-GS) and the low-molecular-weight glutenin (LMW-GS) loci and alleles associated with greater dough strength were identified. Specifically, among the HMW-GS alleles, the Glu-B1i, Glu-B1al, and Glu-D1d alleles were associated with greater mixograph and alveograph values and greater loaf volume. Similarly, among the LMW-GS alleles, the Glu-A3b and Glu-B3b alleles were associated with stronger gluten and better bread-making quality. Overall, results of this study suggest that biofortification does not profoundly alter wheat end-use quality and that the effect of the different glutenin alleles is independent of the grain protein and micronutrient content.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a24bdcc8fa1dcf2b363002f17e5bd9cTest

المؤلفون: Poornima Sharma, Anju Mahendru-Singh, Deepak Ganjewala, Anjali Rai, K. Raghunandan, Sumit K. Singh, R. B. Shukla, Tej Pratap Jitendra Kumar, Arvind Kumar Ahlawat, M. Sivasamy

المصدر: 3 Biotech

مصطلحات موضوعية: Positive selection, food and beverages, Environmental Science (miscellaneous), Biology, Background selection, Agricultural and Biological Sciences (miscellaneous), Negative selection, Horticulture, Backcrossing, Trait, Original Article, Cultivar, Gene, Selection (genetic algorithm), Biotechnology

الوصف: Grain softness has been a major trait of interest in wheat because of its role in producing flour suitable for making high-quality biscuits, cookies, cakes and some other products. In the present study, marker-assisted backcross breeding scheme was deployed to develop advanced wheat lines with soft grains. The Australian soft-grained variety Barham was used as the donor parent to transfer the puroindoline grain softness gene Pina-D1a to the Indian variety, DBW14, which is hard grained and has PinaD1bPinbD1a genes. Foreground selection with allele-specific PCR-based primer for Pina-D1a (positive selection) was used to identify heterozygous BC(1)F(1) plants. Background selection with 173 polymorphic SSR primers covering all the 21 chromosomes was also carried out, in the foreground-selected BC(1)F(1) plants. BC(1)F(2) plants were selected by ascertaining the presence of Pina-D1a (positive selection) and absence of Pina-D1b (negative selection). Using the approach of positive, negative and background selection with molecular markers, 15 BC(1)F(2) and 31 BC(2)F(1) plants were finally selected. The 15 BC(1)F(2) plants were selfed and the 31 BC(2)F(1) plants were further backcrossed and selfed to raise BC(3)F(1) and BC(2)F(2) progenies, respectively. A part of the BC(2)F(2) seed of each of the 31 plants was analyzed for grain hardness index (GHI) with single-kernel characterization system. The GHI varied from 12.1 to 37.1 in the seeds borne on the 31 BC(2)F(1) plants. The reasons for this variation and further course of action are discussed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13205-019-1717-5) contains supplementary material, which is available to authorized users.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4631246819bc5fbab78c08d47e522c67Test
https://doi.org/10.1007/s13205-019-1717-5Test

المؤلفون: Anju Mahendru Singh, Gopalareddy Krishnappa, A. K. Ahlawat, S. K. Singh, Poornima Sharma, R. B. Shukla, G. P. Singh

المصدر: The Indian Journal of Genetics and Plant Breeding (The). 78

مصطلحات موضوعية: food and beverages, chemistry.chemical_element, Plant Science, Marker analysis, Zinc, Biology, Quantitative trait locus, Horticulture, chemistry, Genetic marker, Polymorphism (computer science), Genotype, Genetics, Microsatellite, Plant breeding

الوصف: Markers linked to QTLs are useful in practical plant breeding, only if they get validated in genotypes of independent populations and diverse genetic backgrounds. 41 SSR markers reported linked to QTLs for grain iron (Fe) and zinc (Zn) concentration in wheat were analysed. Only 16 of them showed polymorphism and the remaining 25 turned out to be monomorphic in 48 wheat genotypes used in the present study. Single marker analysis (SMA) for the 16 polymorphic markers was carried out to assess the linkage between marker and the trait, based on which two markers (Xbarc186 and Xbarc74) for grain Fe concentration and three markers (Xgwm3, Xwms149 and Xgwm538) for grain Zn concentration were validated in the present study. The phenotypic variations explained by Xbarc186, Xbarc74, Xgwm3, Xwms149 and Xgwm538 were 40.2% and 19.8%, 10.7%, 21.7%, and 39.6%, respectively. The validation of these SSRs may be useful in breeding wheat with high grain Fe and Zn concentration.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::8142a906032558af25eabf487b2b8a1dTest
https://doi.org/10.31742/ijgpb.78.3.14Test