التفاصيل البيبلوغرافية
| العنوان: |
Lactate-dependent transcriptional regulation controls mammalian eye morphogenesis. |
| المؤلفون: |
Takata, Nozomu, Miska, Jason M., Morgan, Marc A., Patel, Priyam, Billingham, Leah K., Joshi, Neha, Schipma, Matthew J., Dumar, Zachary J., Joshi, Nikita R., Misharin, Alexander V., Embry, Ryan B., Fiore, Luciano, Gao, Peng, Diebold, Lauren P., McElroy, Gregory S., Shilatifard, Ali, Chandel, Navdeep S., Oliver, Guillermo |
| المصدر: |
Nature Communications; 7/14/2023, Vol. 14 Issue 1, p1-17, 17p |
| مصطلحات موضوعية: |
GENETIC transcription regulation, HISTONE acetylation, HISTONE deacetylase, LACTATE dehydrogenase, TRANSCRIPTION factors, MORPHOGENESIS, GLUCOSE transporters |
| مستخلص: |
Mammalian retinal metabolism favors aerobic glycolysis. However, the role of glycolytic metabolism in retinal morphogenesis remains unknown. We report that aerobic glycolysis is necessary for the early stages of retinal development. Taking advantage of an unbiased approach that combines the use of eye organoids and single-cell RNA sequencing, we identify specific glucose transporters and glycolytic genes in retinal progenitors. Next, we determine that the optic vesicle territory of mouse embryos displays elevated levels of glycolytic activity. At the functional level, we show that removal of Glucose transporter 1 and Lactate dehydrogenase A gene activity from developing retinal progenitors arrests eye morphogenesis. Surprisingly, we uncover that lactate-mediated upregulation of key eye-field transcription factors is controlled by the epigenetic modification of histone H3 acetylation through histone deacetylase activity. Our results identify an unexpected bioenergetic independent role of lactate as a signaling molecule necessary for mammalian eye morphogenesis. Using a combination of eye organoids and mouse models the authors identify a bioenergetic independent role of lactate as a cell signaling molecule required during early stages of eye formation in mice. [ABSTRACT FROM AUTHOR] |
|
Copyright of Nature Communications is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder