التفاصيل البيبلوغرافية
| العنوان: |
Cytotoxicity Assessment of Ma-huang (Ephedra) under Different Conditions of Preparation. |
| المؤلفون: |
Lee, M. K., Cheng, B. W. H., Che, C. T., Hsieh, D. P. H. |
| المصدر: |
Toxicological Sciences; Aug2000, Vol. 56 Issue 2, p424-430, 7p, 1 Chart, 5 Graphs |
| مصطلحات موضوعية: |
EPHEDRA, EPHEDRINE, HERBAL medicine, EFFECT of poisons on plants, COLORIMETRY |
| مستخلص: |
Ma-huang is a traditional Chinese medicinal herb derived from Ephedra sinica Stapf and other Ephedra species, used to treat asthma, nose and lung congestion, and fever with anhidrosis. It contains 0.5–2.5% by weight of total alkaloids, of which ephedrine accounts for 30 to 90%. Recently, large amounts of ma-huang were used as a source of ephedrine in many dietary supplements formulated for weight reduction, because ephedrine has been found effective in inducing weight loss in diet-restricted obese patients. However, indiscriminate consumption of ma-huang–containing products has resulted in many cases of poisoning, some of which were fatal. The objective of this study is to investigate the relative toxicity of ma-huang extracted under different conditions. The toxicities of various extracts were assayed using MTT colorimetry on a battery of cell lines, while ephedrine alkaloids were analyzed with HPLC. The results are summarized as follows. (1) The cytotoxicity of all ma-huang extracts could not be totally accounted for by their ephedrine contents, suggesting the presence of other toxins in the extracts. (2) Grinding was a significant condition enhancing the toxicity of the extracts. (3) The relatively high sensitivity of the Neuro-2a cell line to the toxicity of ma-huang extracts suggests that the toxic principles were acting on neuronal cells. (4) One condition to produce a ma-huang extract with high ephedrine-to-toxins ratio would be to boil the whole herb for two h. [ABSTRACT FROM PUBLISHER] |
|
Copyright of Toxicological Sciences is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
