دورية أكاديمية
A proteasome-resistant fragment of NIK mediates oncogenic NF-κB signaling in schwannomas
| العنوان: | A proteasome-resistant fragment of NIK mediates oncogenic NF-κB signaling in schwannomas |
|---|---|
| المؤلفون: | Gehlhausen, Jeffrey R., Hawley, Eric, Wahle, Benjamin Mark, He, Yongzheng, Edwards, Donna, Rhodes, Steven D., Lajiness, Jacquelyn D., Staser, Karl, Chen, Shi, Yang, Xianlin, Yuan, Jin, Li, Xiaohong, Jiang, Li, Smith, Abbi, Bessler, Waylan, Sandusky, George, Stemmer-Rachamimov, Anat, Stuhlmiller, Timothy J., Angus, Steven P., Johnson, Gary L., Nalepa, Grzegorz, Yates, Charles W., Clapp, D. Wade, Park, Su-Jung |
| المساهمون: | Pediatrics, School of Medicine |
| المصدر: | PMC |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2019 |
| المجموعة: | Indiana University - Purdue University Indianapolis: IUPUI Scholar Works |
| مصطلحات موضوعية: | Autocrine Communication, Carcinogenesis, Caspase 1, Cell Proliferation, Disease Models, Animal, Gene Expression Regulation, Neoplastic, Hepatocyte Growth Factor, Molecular Targeted Therapy, NF-kappa B, Neurilemmoma, Neurofibromatosis 2, Proteasome Endopeptidase Complex, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins c-met, Schwann Cells, Signal Transduction |
| الوصف: | Schwannomas are common, highly morbid and medically untreatable tumors that can arise in patients with germ line as well as somatic mutations in neurofibromatosis type 2 (NF2). These mutations most commonly result in the loss of function of the NF2-encoded protein, Merlin. Little is known about how Merlin functions endogenously as a tumor suppressor and how its loss leads to oncogenic transformation in Schwann cells (SCs). Here, we identify nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB)-inducing kinase (NIK) as a potential drug target driving NF-κB signaling and Merlin-deficient schwannoma genesis. Using a genomic approach to profile aberrant tumor signaling pathways, we describe multiple upregulated NF-κB signaling elements in human and murine schwannomas, leading us to identify a caspase-cleaved, proteasome-resistant NIK kinase domain fragment that amplifies pathogenic NF-κB signaling. Lentiviral-mediated transduction of this NIK fragment into normal SCs promotes proliferation, survival, and adhesion while inducing schwannoma formation in a novel in vivo orthotopic transplant model. Furthermore, we describe an NF-κB-potentiated hepatocyte growth factor (HGF) to MET proto-oncogene receptor tyrosine kinase (c-Met) autocrine feed-forward loop promoting SC proliferation. These innovative studies identify a novel signaling axis underlying schwannoma formation, revealing new and potentially druggable schwannoma vulnerabilities with future therapeutic potential. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | Human Molecular Genetics; Gehlhausen, J. R., Hawley, E., Wahle, B. M., He, Y., Edwards, D., Rhodes, S. D., Lajiness, J. D., Staser, K., Chen, S., Yang, X., Yuan, J., Li, X., Jiang, L., Smith, A., Bessler, W., Sandusky, G., Stemmer-Rachamimov, A., Stuhlmiller, T. J., Angus, S. P., Johnson, G. L., … Park, S. J. (2019). A proteasome-resistant fragment of NIK mediates oncogenic NF-κB signaling in schwannomas. Human molecular genetics, 28(4), 572–583. https://doi.org/10.1093/hmg/ddy361Test; https://hdl.handle.net/1805/22494Test |
| الإتاحة: | https://doi.org/10.1093/hmg/ddy361Test https://hdl.handle.net/1805/22494Test |
| حقوق: | Publisher Policy |
| رقم الانضمام: | edsbas.B7F5A6D |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |