Southern Tunisia: A still high endemicity area for hepatitis A
| العنوان: | Southern Tunisia: A still high endemicity area for hepatitis A |
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| المؤلفون: | Anne-Marie Roque-Afonso, Houcine Neffatti, Patricia Lebraud, Jawher Gharbi, Corinne Hottelet, Taieb Challouf |
| المصدر: | PLoS ONE, Vol 12, Iss 4, p e0175887 (2017) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Endemic Diseases, Gastroenterology and hepatology, viruses, Prevalence, lcsh:Medicine, medicine.disease_cause, Hepatitis A Antibodies, Hepatitis, Geographical Locations, Database and Informatics Methods, 0302 clinical medicine, Hepatitis E virus, Pregnancy, Genotype, Medicine, Public and Occupational Health, 030212 general & internal medicine, Sanitation, Pregnancy Complications, Infectious, lcsh:Science, Pathology and laboratory medicine, Phylogeny, Multidisciplinary, biology, Hepatitis A, virus diseases, Medical microbiology, Infectious hepatitis, Viruses, Infectious diseases, RNA, Viral, Female, medicine.symptom, Antibody, Pathogens, Sequence Analysis, Environmental Health, Research Article, Adult, Tunisia, Bioinformatics, 030106 microbiology, Viral diseases, Research and Analysis Methods, Asymptomatic, Microbiology, 03 medical and health sciences, Young Adult, Seroprevalence, Humans, Liver Disease and Pregnancy, Liver diseases, DNA sequence analysis, Medicine and health sciences, Biology and life sciences, business.industry, lcsh:R, Viral pathogens, Organisms, medicine.disease, Virology, Hepatitis viruses, digestive system diseases, Microbial pathogens, Health Care, Immunoglobulin M, People and Places, Africa, biology.protein, lcsh:Q, Hepatitis A virus, business |
| الوصف: | Background Hepatitis A (HAV) and E (HEV) viruses are responsible for enterically transmitted hepatitis. Tunisia is reported to be of intermediate endemicity for HAV and of low seroprevalence for HEV; however, data from rural areas of South Tunisia are lacking. Methods Sera from 216 asymptomatic pregnant women and from 92 patients with acute hepatitis were collected between October 2014 and November 2015. Total and IgM anti-HAV immunoglobulins and anti-HEV IgG and IgM were investigated. Anti-HAV IgM-positive samples were subjected to RT-PCR targeting the VP1/2A region and sequenced. HEV IgM positive samples and all samples from acute hepatitis patients were assessed for HEV RNA. Results Among pregnant women (mean age 32+/-8), HAV seroprevalence was 98.6%, none presented anti-HAV IgM; HEV seroprevalence was 5.1% and three presented weakly reactive anti-HEV IgM without detectable RNA. Among acute hepatitis patients (mean age 18.5 +/- 14), HEV seroprevalence was 19,5%, none presented anti-HEV IgM, nor HEV RNA. HAV seroprevalence exceeded 90% by age 5 and acute HAV infection was detected in 20 patients (21,7%), younger than patients with other hepatitis causes (9,8 years vs. 20,4 years, p = 0,004); 65% were male. Most acute HAV infections were observed in a coastal area where HAV infections represented 52% of hepatitis etiology. Phylogenetic analysis identified genotype IA strains, clustering close to previously published Tunisian sequences. Conclusion The present study confirmed a low HEV endemicity and evidenced a still high level of HAV circulation in Southern Tunisia, suggesting distinct dissemination patterns for these viruses. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::507f5f8ae6cd4698b71779ad82d67d05Test http://europepmc.org/articles/PMC5398567?pdf=renderTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....507f5f8ae6cd4698b71779ad82d67d05 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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