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1
المصدر: Methods in molecular biology (Clifton, N.J.). 2288
مصطلحات موضوعية: Crops, Agricultural, Indoles, Ploidies, Staining and Labeling, Homozygote, Flowers, Haploidy, Diploidy, Culture Media, Tissue Culture Techniques, Plant Breeding, Pollen, Regeneration, Solanum melongena, Molecular Biology, Fluorescent Dyes
الوصف: Eggplant is one of the five important, worldwide-distributed solanaceous crops. The use of anther culture technology to produce pure, 100% homozygous doubled haploid lines for hybrid seed production is possible since 1982, where the first protocol of wide application to different eggplant materials was published. From then on, different improvements and adaptations to different materials have been made. In parallel, protocols to implement isolated microspore culture technology in eggplant have been developed principally in the last decade, which opens the door for a more efficient DH production in this species. In this chapter, two protocols, one for anther and other for isolated microspore culture in eggplant, are described. Some steps and materials are common to both approaches. A detailed description of each step from is provided.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::9da1eb32802c332e2422e82b2805cc13Test
https://pubmed.ncbi.nlm.nih.gov/34270015Test -
2
المصدر: Methods in molecular biology (Clifton, N.J.). 2122
مصطلحات موضوعية: Tissue Culture Techniques, Plant Breeding, Ploidies, DNA, Plant, Plant Development, Pollen, Solanum melongena, Haploidy
الوصف: For a long time, conventional breeding methods have been used to obtain pure, 100% homozygous lines for hybrid seed production in crops of agronomic interest. However, by doubled haploid technology, it is possible to produce 100% homozygous plants derived from precursors of male gametophytes (androgenesis), to accelerate the production of pure lines, which implies important time and cost savings. In this chapter, a protocol for anther culture in eggplant is described, from donor plant growth conditions to regeneration and acclimation of doubled haploid plants, as well as a description of how to analyze ploidy levels of regenerated plants.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::4b3752e51f1ca51894d05ef4bfcc789fTest
https://pubmed.ncbi.nlm.nih.gov/31975310Test -
3
المصدر: Methods in molecular biology (Clifton, N.J.). 2122
مصطلحات موضوعية: Tissue Culture Techniques, DNA, Plant, Acclimatization, Brassica napus, Pollen, Germination, Haploidy
الوصف: Isolated microspore culture is the most efficient technique among those used to induce microspore embryogenesis. In the particular case of Brassica napus, it is also the most widely used and optimized. In this chapter, we describe a protocol for microspore culture in B. napus which includes the steps necessary to isolate and culture microspores, to induce microspore-derived embryos, to produce doubled haploid plants from them, as well as to check for the developmental stage of the microspores isolated, their viability, and the ploidy level of regenerated plantlets.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::19789778e8aa946c7f60166d21499106Test
https://pubmed.ncbi.nlm.nih.gov/31975309Test -
4
المؤلفون: Verónica, Parra-Vega, Jose M, Seguí-Simarro
المصدر: Methods in molecular biology (Clifton, N.J.). 1359
مصطلحات موضوعية: Plant Somatic Embryogenesis Techniques, Tissue Culture Techniques, Plant Development, Pollen, Regeneration, Germination, Flowers, Haploidy, Capsicum
الوصف: Anther culture is the most popular of the techniques used to induce microspore embryogenesis. This technique is well set up in a wide range of crops, including pepper. In this chapter, a protocol for anther culture in pepper is described. The protocol presented hereby includes the steps from the selection of buds from donor plants to the regeneration and acclimatization of doubled haploid plants derived from the embryos, as well as a description of how to analyze the ploidy level of the regenerated plants.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::80ce9eff090c1d4adb1ca68c60ad210dTest
https://pubmed.ncbi.nlm.nih.gov/26619881Test -
5
المؤلفون: Verónica Parra-Vega, Beatriz González-García, Patricia Corral-Martínez, Jose M. Seguí-Simarro
المصدر: RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instnameمصطلحات موضوعية: Plant Science, Review, Haploidy, Embryo development, Tissue Culture Techniques, Microspore, Solanum lycopersicum, Ploidy, Plant Gametogenesis, Aubergine, Gametogenesis, Plant, biology, Biological modeling, Homozygote, General Medicine, Hybrid seed, Plant chromosome, Flower, Plant biochemistry, Seeds, Doubled haploid, Pollen, Capsicum, Solanaceae, Biotechnology, Anther culture, Plant gametogenesis, Growth, development and aging, Embryonic Development, Flowers, Eggplant, Tomato, Chromosomes, Plant, Pepper, Genetics, Microspore culture, Regeneration, Solanum melongena, Lycopersicon esculentum, Ploidies, Models, Genetic, business.industry, Methodology, biology.organism_classification, Drug effect, GENETICA, Tissue culture technique, Doubled haploidy, Biological model, business, Cytology, Agronomy and Crop Science
الوصف: [EN] Tomato, eggplant, and pepper are three solanaceous crops of outstanding importance worldwide. For hybrid seed production in these species, a fast and cheap method to obtain pure (homozygous) lines is a priority. Traditionally, pure lines are produced by classical inbreeding and selection techniques, which are time consuming (several years) and costly. Alternatively, it has become possible to accelerate the production of homozygous lines through a biotechnological approach: the induction of androgenesis to generate doubled haploid (homozygous) plants. This biotechnological in vitro tool reduces the process to only one generation, which implies important time and costs savings. These facts make androgenic doubled haploids the choice in a number of important crops where the methodology is well set up. Unfortunately, recalcitrant solanaceous crops such as tomato, eggplant, and pepper are still far from an efficient and reliable technology to be applied on a routine basis to different genotypes in breeding programs. In eggplant and pepper, only anther cultures are known to work relatively well. Unfortunately, a more efficient and promising technique, the culture of isolated microspores, is not sufficiently developed yet. In tomato, none of these methods is available nowadays. However, recent advances in the knowledge of embryo development are filling the gaps and opening new ways to achieve the final goal of an efficient protocol in these three recalcitrant species. In this review, we outline the state of the art on androgenic induction in tomato, eggplant, and pepper, and postulate new experimental ways in order to overcome current limitations. © 2010 Springer-Verlag.
Authors want to express their thanks to Mrs. Nuria Palacios, Dr. Antonio Serrano and Dr. Begona Renau for their excellent technical help. This work was supported by grants AGL2006-06678 and AGL2010-17895 from the Spanish Ministry of Science and Innovation (MICINN) to JMSS.وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d776e22d87e5145d32b65aa4ead1ca23Test
https://pubmed.ncbi.nlm.nih.gov/21191595Test
