Functionalizing Collagen Membranes with MSC-Conditioned Media Promotes Guided Bone Regeneration in Rat Calvarial Defects
| العنوان: | Functionalizing Collagen Membranes with MSC-Conditioned Media Promotes Guided Bone Regeneration in Rat Calvarial Defects |
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| المؤلفون: | Shanbhag, Siddharth, Kampleitner, Carina, Al-Sharabi, Niyaz, Mohamed-Ahmed, Samih, Apaza Alccayhuaman, Karol Ali, Heimel, Patrick, Tangl, Stefan, Beinlich, Andreas, Rana, Neha, Sanz, Mariano, Kristoffersen, Einar K, Mustafa, Kamal, Gruber, Reinhard |
| المصدر: | Cells Volume 12 Issue 5 Pages: 767 Shanbhag, Siddharth; Kampleitner, Carina; Al-Sharabi, Niyaz; Mohamed-Ahmed, Samih; Apaza Alccayhuaman, Karol Ali; Heimel, Patrick; Tangl, Stefan; Beinlich, Andreas; Rana, Neha; Sanz, Mariano; Kristoffersen, Einar K; Mustafa, Kamal; Gruber, Reinhard (2023). Functionalizing Collagen Membranes with MSC-Conditioned Media Promotes Guided Bone Regeneration in Rat Calvarial Defects. Cells, 12(5) MDPI 10.3390/cells12050767 <http://dx.doi.org/10.3390/cells12050767Test> |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | guided bone regeneration, conditioned media, regenerative medicine, 610 Medicine & health, General Medicine, mesenchymal stromal cells, bone tissue engineering |
| الوصف: | Functionalizing biomaterials with conditioned media (CM) from mesenchymal stromal cells (MSC) is a promising strategy for enhancing the outcomes of guided bone regeneration (GBR). This study aimed to evaluate the bone regenerative potential of collagen membranes (MEM) functionalized with CM from human bone marrow MSC (MEM-CM) in critical size rat calvarial defects. MEM-CM prepared via soaking (CM-SOAK) or soaking followed by lyophilization (CM-LYO) were applied to critical size rat calvarial defects. Control treatments included native MEM, MEM with rat MSC (CEL) and no treatment. New bone formation was analyzed via micro-CT (2 and 4 weeks) and histology (4 weeks). Greater radiographic new bone formation occurred at 2 weeks in the CM-LYO group vs. all other groups. After 4 weeks, only the CM-LYO group was superior to the untreated control group, whereas the CM-SOAK, CEL and native MEM groups were similar. Histologically, the regenerated tissues showed a combination of regular new bone and hybrid new bone, which formed within the membrane compartment and was characterized by the incorporation of mineralized MEM fibers. Areas of new bone formation and MEM mineralization were greatest in the CM-LYO group. Proteomic analysis of lyophilized CM revealed the enrichment of several proteins and biological processes related to bone formation. In summary, lyophilized MEM-CM enhanced new bone formation in rat calvarial defects, thus representing a novel ‘off-the-shelf’ strategy for GBR. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2073-4409 |
| DOI: | 10.3390/cells12050767 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63458b774f5f9ac72760dc542ecdf422Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....63458b774f5f9ac72760dc542ecdf422 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734409 |
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| DOI: | 10.3390/cells12050767 |