The Role of Trs65 in the Ypt/Rab Guanine Nucleotide Exchange Factor Function of the TRAPP II Complex
| العنوان: | The Role of Trs65 in the Ypt/Rab Guanine Nucleotide Exchange Factor Function of the TRAPP II Complex |
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| المؤلفون: | Nava Segev, Nadya Morozova, Jonathan W. Mulholland, Yongheng Liang, Andrei A. Tokarev |
| المصدر: | Molecular Biology of the Cell. 18:2533-2541 |
| بيانات النشر: | American Society for Cell Biology (ASCB), 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Saccharomyces cerevisiae Proteins, Protein subunit, Saccharomyces cerevisiae, Vesicular Transport Proteins, Golgi Apparatus, Plasma protein binding, Models, Biological, symbols.namesake, Guanine Nucleotide Exchange Factors, Molecular Biology, biology, Membrane Proteins, Articles, Cell Biology, Golgi apparatus, biology.organism_classification, Transport protein, Cell biology, Protein Subunits, Protein Transport, Phenotype, TRAPP complex, Biochemistry, rab GTP-Binding Proteins, Mutation, symbols, biology.protein, Guanine nucleotide exchange factor, Rab, Protein Binding |
| الوصف: | The conserved modular complex TRAPP is a guanine nucleotide exchanger (GEF) for the yeast Golgi Ypt-GTPase gatekeepers. TRAPP I and TRAPP II share seven subunits and act as GEFs for Ypt1 and Ypt31/32, respectively, which in turn regulate transport into and out of the Golgi. Trs65/Kre11 is one of three TRAPP II-specific subunits. Unlike the other two subunits, Trs120 and Trs130, Trs65 is not essential for viability, is conserved only among some fungi, and its contribution to TRAPP II function is unclear. Here, we provide genetic, biochemical, and cellular evidence for the role of Trs65 in TRAPP II function. First, like Trs130, Trs65 localizes to the trans-Golgi. Second, TRS65 interacts genetically with TRS120 and TRS130. Third, Trs65 interacts physically with Trs120 and Trs130. Finally, trs65 mutant cells have low levels of Trs130 protein, and they are defective in the GEF activity of TRAPP II and the intracellular distribution of Ypt1 and Ypt31/32. Together, these results show that Trs65 plays a role in the Ypt GEF activity of TRAPP II in concert with the two other TRAPP II-specific subunits. Elucidation of the role played by Trs65 in intracellular trafficking is important for understanding how this process is coordinated with two other processes in which Trs65 is implicated: cell wall biogenesis and stress response. |
| تدمد: | 1939-4586 1059-1524 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fec5271cd8458b549208e4f17d45130Test https://doi.org/10.1091/mbc.e07-03-0221Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0fec5271cd8458b549208e4f17d45130 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19394586 10591524 |
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