Mesenteric Lymph Node Transplantation in Mice to Study Immune Responses of the Gastrointestinal Tract
| العنوان: | Mesenteric Lymph Node Transplantation in Mice to Study Immune Responses of the Gastrointestinal Tract |
|---|---|
| المؤلفون: | Shaikh, Haroon, Vargas, Juan Gamboa, Mokhtari, Zeinab, Jarick, Katja J., Ulbrich, Maria, Mosca, Josefina Peña, Viera, Estibaliz Arellano, Graf, Caroline, Le, Duc-Dung, Heinze, Katrin G., Büttner-Herold, Maike, Rosenwald, Andreas, Pezoldt, Joern, Huehn, Jochen, Beilhack, Andreas |
| المساهمون: | HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany. |
| المصدر: | Frontiers in immunology Switzerland Frontiers in Immunology |
| بيانات النشر: | Frontiers, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | lymph node stromal cells, acute graft-versus host disease, alloreactive T cells, T-Lymphocytes, Immunology, lymph node transplantation, Graft vs Host Disease, Lymphocyte Activation, mesenteric lymph node, Methods, Animals, Mesentery, mouse models, ddc:610, Cell Proliferation, Graft Survival, Hematopoietic Stem Cell Transplantation, Histocompatibility Antigens Class II, Cell Differentiation, Adoptive Transfer, Mice, Inbred C57BL, Disease Models, Animal, Transplantation, Isogeneic, Phenotype, surgical procedures, operative, Acute Disease, Transplantation Tolerance, Lymph Nodes |
| الوصف: | Mesenteric lymph nodes (mLNs) are sentinel sites of enteral immunosurveillance and immune homeostasis. Immune cells from the gastrointestinal tract (GIT) are constantly recruited to the mLNs in steady-state and under inflammatory conditions resulting in the induction of tolerance and immune cells activation, respectively. Surgical dissection and transplantation of lymph nodes (LN) is a technique that has supported seminal work to study LN function and is useful to investigate resident stromal and endothelial cell biology and their cellular interactions in experimental disease models. Here, we provide a detailed protocol of syngeneic mLN transplantation and report assays to analyze effective mLN engraftment in congenic recipients. Transplanted mLNs allow to study T cell activation and proliferation in preclinical mouse models. Donor mLNs proved viable and functional after surgical transplantation and regenerated blood and lymphatic vessels. Immune cells from the host completely colonized the transplanted mLNs within 7-8 weeks after the surgical intervention. After allogeneic hematopoietic cell transplantation (allo-HCT), adoptively transferred allogeneic CD4+ T cells from FVB/N (H-2q) mice homed to the transplanted mLNs in C57BL/6 (H-2b) recipients during the initiation phase of acute graft-versus-host disease (aGvHD). These CD4+ T cells retained full proliferative capacity and upregulated effector and gut homing molecules comparable to those in mLNs from unmanipulated wild-type recipients. Wild type mLNs transplanted into MHCII deficient syngeneic hosts sufficed to activate alloreactive T cells upon allogeneic hematopoietic cell transplantation, even in the absence of MHCII+ CD11c+ myeloid cells. These data support that orthotopically transplanted mLNs maintain physiological functions after transplantation. The technique of LN transplantation can be applied to study migratory and resident cell compartment interactions in mLNs as well as immune reactions from and to the gut under inflammatory and non-inflammatory conditions. |
| وصف الملف: | application/pdf; image/tiff |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::444e39528ff1f56b0b3507eada99c798Test https://hdl.handle.net/10033/623028Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....444e39528ff1f56b0b3507eada99c798 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |