المؤلفون: Laura Mugnai, V. Romano, S. Raspanti, M. G. Di Bello, Emanuela Masini, P. F. Mannaioni

المصدر: Inflammation research : official journal of the European Histamine Research Society ... [et al.]. 45

مصطلحات موضوعية: Narcotics, Allergy, Free Radicals, Immunology, Pharmacology, Histamine Release, Cyclic N-Oxides, chemistry.chemical_compound, Cocaine, Lactate dehydrogenase, Malondialdehyde, Oxidative enzyme, medicine, Animals, Vitamin E, Centrifugation, Fluorometry, Mast Cells, Rats, Wistar, Peritoneal Cavity, Chromatography, Dose-Response Relationship, Drug, L-Lactate Dehydrogenase, Morphine, Chemistry, Illicit Drugs, Free Radical Scavengers, Mast cell, medicine.disease, Glutathione, Rats, medicine.anatomical_structure, Spectrophotometry, Pleura, Phenobarbital, Histamine, Methadone, medicine.drug

الوصف: (Sorensen buffer, pH7.4, 37°C) with (a) cocaine, morphine and methadone, (b) with the oxidative enzymes, and (c) with cocaine, morphine and methadone in the presence of oxidative enzymes. The oxidative enzymes were PHS purified from ram seminal vesic1es (20 U/mg), and SIO Mix liver microsomes from phenobarbital treated rats (4 mg/mi protein). The reaction was stopped by chilling the tubes at 0-4 oe. After centrifugation, histamine and lactate dehydrogenase (LDH) were analysed in the supernatants and in the pellets (fluorimetric and spectrophotometric assays, respectively) and expressed as percent of the total histamine content. MDA was measured by a spectrophotometric assay [4].

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dce2fa42bb67e5b4bbca1936ea889cd0Test
https://pubmed.ncbi.nlm.nih.gov/8696914Test

المؤلفون: C Ioannides, P. F. Mannaioni, M. G. Di Bello, S. Raspanti, J. F. Ndisang, Tatiana Bani Sacchi, Emanuela Masini

المصدر: Inflammation Research. 62:247-247

مصطلحات موضوعية: Pharmacology, Lysis, Immunology, Glutathione, Mast cell, chemistry.chemical_compound, medicine.anatomical_structure, chemistry, Lactate dehydrogenase, Oxidative enzyme, medicine, Morphine, Secretion, Histamine, medicine.drug

الوصف: Background: The metabolic activation of morphine, cocaine and methadone into free radicals could have pathophysiological relevance in the organic injuries of drug addiction.¶Methods: Isolated purified rat serosal mast cells were incubated with morphine, cocaine and methadone (10−7 M–10−4 M) with oxidative enzymes (prostaglandin-H-synthetase, 25 mU; rat liver homogenate fraction S 10-mix, 400 μl), and with the drugs of abuse in the presence of oxidative enzymes. Histamine and lactate dehydrogenase (LDH) were analysed with a fluorimetric and spectrophotometric assay, respectively; the generation of malonyldialdehyde (MDA) was measured by a spectrophotometric assay.¶Results: The release of mast cell histamine and the generation of MDA are present only when mast cells were incubated with the drugs of abuse in the presence of oxidative enzymes. This release was dependent on the concentration of the drug in question and showed a maximum value at 10−4 M. Moreover, in parallel experiments we demonstrated that, under the same experimental conditions, the release of LDH was always less than 20% of the total, suggesting that this effect is due to a selective exocytotic process. Histamine release and MDA generation were abated by the free radical scavengers: reduced glutathione, 10−4 M GSH and α-tocopherol, 10−4 M and by the spin trapper 5,5-dimethyl-l-pyrroline-N-oxide, 10−4 M DMPO. The light and electron microscopic features are consistent with exocytotic secretion in the cases of morphine and methadone and with cell lysis in the case of cocaine.¶Conclusion: These results suggest that morphine, cocaine and methadone are activated into free radicals which produce membrane lipid perturbation and histamine release, suggesting that a massive release of mast cell histamine could be an additional risk factor in heroin and cocaine overdoses.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::9d45898c65b575c3cfad85520d5c39bcTest
https://doi.org/10.1007/s00011-012-0582-zTest

المؤلفون: S. Raspanti, V. Romano, Laura Mugnai, Pier Francesco Mannaioni, Emanuela Masini, M. G. Di Bello

المصدر: Modulation of Cellular Responses in Toxicity ISBN: 9783642798740

مصطلحات موضوعية: Drug, Chemistry, media_common.quotation_subject, Glutathione, Pharmacology, Mast cell, chemistry.chemical_compound, medicine.anatomical_structure, Oxidative enzyme, Toxicity, medicine, Morphine, Histamine, Methadone, medicine.drug, media_common

الوصف: 1. Isolated purified rat serosal mast cells were incubated with given concentrations of drugs of abuse (morphine, cocaine, methadone), with oxidative enzymes (prostaglandin-H-synthetase, PHS; rat liver homogenate fraction - S-10 mix), and with the drugs of abuse in the presence of oxidative enzymes. 2. The release of mast cell histamine and the generation of malonyl-dialdehyde (MDA) is present only when mast cells were incubated with the drugs of abuse in the presence of oxidative enzymes. 3. The release of histamine and the generation of MDA were abated by the free radical scavengers, reduced glutathione (GSH) and α-tocopherol. 4. It is suggested that morphine, cocaine and methadone are activated into free radicals producing membrane lipid perturbation and histamine release. 5. The metabolic activation of morphine, cocaine and methadone into free radicals could entail pathophysiological relevance in the organic injuries of drug addiction.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::c3d7bbc3adab1226aff13596cf7e7778Test
https://doi.org/10.1007/978-3-642-79872-6_12Test

المؤلفون: A, Pistelli, P, Di Simplicio, M G, Di Bello, S, Raspanti, F, Gambassi, P, Botti, L, Caramelli, S, Peruzzi, C, Smorlesi, A M, Zorn

المصدر: La Clinica terapeutica. 140(5)

مصطلحات موضوعية: Adult, Male, Alcoholism, Glucaric Acid, Erythrocytes, Time Factors, Humans, Chlordiazepoxide, Female, gamma-Glutamyltransferase, Middle Aged, Glutathione

الوصف: The effects of reduced glutathione (GSH) administration (1.2 g/day and 2.4 g/day intravenously) on erythrocyte glutathione levels, serum gamma-glutamyl transpeptidase activity (GGTP) and urinary glucaric acid elimination were studied in a population of 24 chronic alcoholics voluntarily admitted to a 30 day detoxification protocol in comparison to a 12 patient control group treated only with chlordiazepoxide (initial dose 75-100 mg/day). Glutathione treatment increases dose-dependently and in a significant way erythrocyte glutathione levels and hastens the recovery of serum GGTP and urinary glucaric acid elimination. The relationship between glutathione, GGTP and glucaric acid is discussed, suggesting the possible role of GSH against the oxidative damage of alcohol.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::6c3e4424fc51b7734107a624be531febTest
https://pubmed.ncbi.nlm.nih.gov/1358498Test