دورية أكاديمية
Intrinsic expression of transcortin in neural cells of the mouse brain: a histochemical and molecular study
| العنوان: | Intrinsic expression of transcortin in neural cells of the mouse brain: a histochemical and molecular study |
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| المؤلفون: | Sivukhina, Elena, Helbling, Jean-Christophe, Minni, Amandine, Schafer, Hendrik H., Pallet, Véronique, Jirikowski, Gustav F., Moisan, Marie-Pierre |
| المساهمون: | Friedrich-Schiller-Universität = Friedrich Schiller University Jena Jena, Germany, Nutrition et Neurobiologie intégrée (NutriNeuro), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS), We wish to thank The Journal of Experimental Biology and The Company of Biologists Ltd (Travelling Fellowship), and ‘Boehringer Ingelheim Foundation’ for their financial support to E.S. for part of this work. |
| المصدر: | ISSN: 0022-0949. |
| بيانات النشر: | HAL CCSD The Company of Biologists |
| سنة النشر: | 2013 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | corticosteroid-binding globulin, transcortin, glucocorticoid, brain, glial cell, [SDV]Life Sciences [q-bio] |
| الوصف: | Chantier qualité GA ; International audience ; Corticosteroid binding globulin (CBG, transcortin) has been shown to be expressed in the brain of rat and human species. In this study, we examined the CBG brain expression and cDNA structure in mice, comparing wild-type (Cbg(+/+)) and Cbg knockout mice (Cbg(-/-), obtained by genetic disruption of the SerpinA6 alias Cbg gene). We used double immunofluorescence labeling with specific neuronal and glial markers to analyze the cellular localization of CBG in various regions of the mouse brain. In wild-type (Cbg(+/+)) mice, we found CBG immunoreactivity in neuronal perikarya of the magnocellular hypothalamic nuclei, amygdala, hippocampus, cerebral cortex, cerebellum and pituitary. A portion of glial cells (astrocytes, oligodendrocytes) contained CBG immunoreactivity, including some of the ependymal cells and choroid plexus cells. No CBG immunoreactivity was detected in Cbg(-/-) brain tissues. Using RT-PCR, we showed that the full-length Cbg mRNA is present in those regions, indicating an intrinsic expression of the steroid-binding globulin. Furthermore, sequencing analysis showed that Cbg cDNA obtained from the mouse hypothalamus was homologous to Cbg cDNA obtained from the liver. Finally, we have evaluated the relative levels of CBG expression in various brain regions and in the liver by quantitative PCR. We found that brain levels of Cbg mRNA are low compared with the liver but significantly higher than in CBG-deficient mice. Although derived from the same gene as liver CBG, brain CBG protein may play a specific or complementary role that requires the production and analysis of brain-specific Cbg knockout models. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-02646029; https://hal.inrae.fr/hal-02646029Test; PRODINRA: 184316; WOS: 000312735700021 |
| DOI: | 10.1242/jeb.076893 |
| الإتاحة: | https://doi.org/10.1242/jeb.076893Test https://hal.inrae.fr/hal-02646029Test |
| رقم الانضمام: | edsbas.427B16DA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1242/jeb.076893 |
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