دورية أكاديمية
Myocilin expression is regulated by retinoic acid in the trabecular meshwork-derived cellular environment
| العنوان: | Myocilin expression is regulated by retinoic acid in the trabecular meshwork-derived cellular environment |
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| المؤلفون: | Prat, Cécile, Belville, Corinne, Comptour, Aurélie, Marceau, Geoffroy, Clairefond, Gael, Chiambaretta, Frédéric, Sapin, Vincent, Blanchon, Loïc |
| المساهمون: | Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne 2017-2020 (UCA 2017-2020 )-Centre National de la Recherche Scientifique (CNRS), Laoratoire de Biochimie, CHU Clermont-Ferrand, CHU Gabriel Montpied Clermont-Ferrand, Laboratoire de Biochimie, Génétique, Reproduction et Développement (GReD ) |
| المصدر: | ISSN: 0014-4835. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2017 |
| المجموعة: | HAL Clermont Auvergne (Université Blaise Pascal Clermont-Ferrand / Université d'Auvergne) |
| مصطلحات موضوعية: | Gene regulation, Glaucoma, Myocilin, Retinoic acid, Retinoids, Trabecular meshwork, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs |
| الوصف: | International audience ; Glaucoma is the leading cause of irreversible blindness and is usually classified as angle closure and open angle glaucoma (OAG). Primary open angle glaucoma represents the most frequent clinical presentation leading to ganglion cell death and optic nerve degeneration as a main consequence of an intraocular pressure' (IOP) increase. The mechanisms of this IOP increase in such pathology remain unclear but one protein called Myocilin could be a part of the puzzle in the trabecular meshwork (TM). Previously described to be transcriptionally regulated by glucocorticoids, the comprehension of the trabecular regulation of Myocilin' expression has only weakly progressed since 15 years. Due to the essential molecular and cellular implications of retinoids' pathway in eye development and physiology, we investigate the potential role of the retinoic acid in such regulation and expression. This study demonstrates that the global retinoids signaling machinery is present in immortalized TM cells and that Myocilin (MYOC) expression is upregulated by retinoic acid alone or combined with a glucocorticoid co-treatment. This regulation by retinoic acid acts through the MYOC promoter which contains a critical cluster of four retinoic acid responsive elements (RAREs), with the RARE-DR2 presenting the strongest effect and binding the RARα/RXRα heterodimer. All together, these results open up new perspectives for the molecular understanding glaucoma pathophysiology and provide further actionable clues on Myocilin gene regulation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/28153738; hal-01918671; https://uca.hal.science/hal-01918671Test; PUBMED: 28153738 |
| DOI: | 10.1016/j.exer.2017.01.006 |
| الإتاحة: | https://doi.org/10.1016/j.exer.2017.01.006Test https://uca.hal.science/hal-01918671Test |
| رقم الانضمام: | edsbas.BFA041C4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.exer.2017.01.006 |
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