A novel ATG5 interaction with Ku70 potentiates DNA repair upon genotoxic stress
| العنوان: | A novel ATG5 interaction with Ku70 potentiates DNA repair upon genotoxic stress |
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| المؤلفون: | Sinem Demirbag-Sarikaya, Yunus Akkoc, Sıla Turgut, Secil Erbil-Bilir, Nur Mehpare Kocaturk, Joern Dengjel, Devrim Gozuacik |
| المساهمون: | Gözüaçık, Devrim (ORCID 0000-0001-7739-2346 & YÖK ID 40248), Akkoç, Yunus, Turgut, Sıla, Demirbağ Sarıkaya, Erbil Bilir, S., Kocatürk, N.M., Dengjel, J., Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM), School of Medicine, Graduate School of Health Sciences |
| المصدر: | Scientific Reports |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | DNA-Binding Proteins, Multidisciplinary, Genotoxic stress, DNA repair, DNA End-Joining Repair, DNA Repair, Humans, Antigens, Nuclear, Ku Autoantigen, Science and technology, Genomic Instability, Autophagy-Related Protein 5, DNA Damage |
| الوصف: | The maintenance of cellular homeostasis in living organisms requires a balance between anabolic and catabolic reactions. Macroautophagy (autophagy herein) is determined as one of the major catabolic reactions. Autophagy is an evolutionarily conserved stress response pathway that is activated by various insults including DNA damage. All sorts of damage to DNA potentially cause loss of genetic information and trigger genomic instability. Most of these lesions are repaired by the activation of DNA damage response following DNA repair mechanisms. Here we describe, a novel protein complex containing the autophagy protein ATG5 and the non-homologous end-joining repair system proteins. We discovered for the frst time that ATG5 interacted with both Ku80 (XRCC5) and Ku70 (XRCC6). This novel interaction is facilitated mainly via Ku70. Our results suggest that this interaction is dynamic and enhanced upon genotoxic stresses. Strikingly, we identifed that ATG5-Ku70 interaction is necessary for DNA repair and efective recovery from genotoxic stress. Therefore, our results are demonstrating a novel, direct, dynamic, and functional interaction between ATG5 and Ku70 proteins that plays a crucial role in DNA repair under genotoxic stress conditions. Scientifc and Technological Research Council of Turkey (TÜBİTAK) |
| وصف الملف: | |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e33f9315c8990950fbea214c282bb88Test https://pubmed.ncbi.nlm.nih.gov/35581289Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0e33f9315c8990950fbea214c282bb88 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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