دورية أكاديمية

Genome-Based Analysis of Verticillium Polyketide Synthase Gene Clusters

التفاصيل البيبلوغرافية
العنوان: Genome-Based Analysis of Verticillium Polyketide Synthase Gene Clusters
المؤلفون: Mohammad Sayari, Aria Dolatabadian, Mohamed El-Shetehy, Pawanpuneet Kaur Rehal, Fouad Daayf
المصدر: Biology, Vol 11, Iss 9, p 1252 (2022)
بيانات النشر: MDPI AG, 2022.
سنة النشر: 2022
المجموعة: LCC:Biology (General)
مصطلحات موضوعية: Verticillium, PKS, cluster, genomics, phylogeny, Biology (General), QH301-705.5
الوصف: Polyketides are structurally diverse and physiologically active secondary metabolites produced by many organisms, including fungi. The biosynthesis of polyketides from acyl-CoA thioesters is catalyzed by polyketide synthases, PKSs. Polyketides play roles including in cell protection against oxidative stress, non-constitutive (toxic) roles in cell membranes, and promoting the survival of the host organisms. The genus Verticillium comprises many species that affect a wide range of organisms including plants, insects, and other fungi. Many are known as causal agents of Verticillium wilt diseases in plants. In this study, a comparative genomics approach involving several Verticillium species led us to evaluate the potential of Verticillium species for producing polyketides and to identify putative polyketide biosynthesis gene clusters. The next step was to characterize them and predict the types of polyketide compounds they might produce. We used publicly available sequences from ten species of Verticillium including V. dahliae, V. longisporum, V. nonalfalfae, V. alfalfae, V. nubilum, V. zaregamsianum, V. klebahnii, V. tricorpus, V. isaacii, and V. albo-atrum to identify and characterize PKS gene clusters by utilizing a range of bioinformatic and phylogenetic approaches. We found 32 putative PKS genes and possible clusters in the genomes of Verticillium species. All the clusters appear to be complete and functional. In addition, at least five clusters including putative DHN-melanin-, cytochalasin-, fusarielien-, fujikurin-, and lijiquinone-like compounds may belong to the active PKS repertoire of Verticillium. These results will pave the way for further functional studies to understand the role of these clusters.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2079-7737
العلاقة: https://www.mdpi.com/2079-7737/11/9/1252Test; https://doaj.org/toc/2079-7737Test
DOI: 10.3390/biology11091252
الوصول الحر: https://doaj.org/article/cd2cb1baac8147b09393cf5a47297b86Test
رقم الانضمام: edsdoj.2cb1baac8147b09393cf5a47297b86
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20797737
DOI:10.3390/biology11091252