التفاصيل البيبلوغرافية
| العنوان: |
BCL-XL Overexpression Protects Pancreatic β-Cells against Cytokine- and Palmitate-Induced Apoptosis. |
| المؤلفون: |
Perez-Serna, Atenea A.1,2 (AUTHOR), Dos Santos, Reinaldo S.1,2 (AUTHOR), Ripoll, Cristina1,2 (AUTHOR), Nadal, Angel1,2 (AUTHOR), Eizirik, Decio L.3 (AUTHOR), Marroqui, Laura1,2 (AUTHOR) lmarroqui@umh.es |
| المصدر: |
International Journal of Molecular Sciences. Mar2023, Vol. 24 Issue 6, p5657. 18p. |
| مصطلحات موضوعية: |
*GENETIC overexpression, *FREE fatty acids, *BCL-2 proteins, *APOPTOSIS, *CELL death |
| مستخلص: |
Diabetes is a chronic disease that affects glucose metabolism, either by autoimmune-driven β-cell loss or by the progressive loss of β-cell function, due to continued metabolic stresses. Although both α- and β-cells are exposed to the same stressors, such as proinflammatory cytokines and saturated free fatty acids (e.g., palmitate), only α-cells survive. We previously reported that the abundant expression of BCL-XL, an anti-apoptotic member of the BCL-2 family of proteins, is part of the α-cell defense mechanism against palmitate-induced cell death. Here, we investigated whether BCL-XL overexpression could protect β-cells against the apoptosis induced by proinflammatory and metabolic insults. For this purpose, BCL-XL was overexpressed in two β-cell lines—namely, rat insulinoma-derived INS-1E and human insulin-producing EndoC-βH1 cells—using adenoviral vectors. We observed that the BCL-XL overexpression in INS-1E cells was slightly reduced in intracellular Ca2+ responses and glucose-stimulated insulin secretion, whereas these effects were not observed in the human EndoC-βH1 cells. In INS-1E cells, BCL-XL overexpression partially decreased cytokine- and palmitate-induced β-cell apoptosis (around 40% protection). On the other hand, the overexpression of BCL-XL markedly protected EndoC-βH1 cells against the apoptosis triggered by these insults (>80% protection). Analysis of the expression of endoplasmic reticulum (ER) stress markers suggests that resistance to the cytokine and palmitate conferred by BCL-XL overexpression might be, at least in part, due to the alleviation of ER stress. Altogether, our data indicate that BCL-XL plays a dual role in β-cells, participating both in cellular processes related to β-cell physiology and in fostering survival against pro-apoptotic insults. [ABSTRACT FROM AUTHOR] |
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