دورية أكاديمية
A second-generation anchored genetic linkage map of the tammar wallaby (Macropus eugenii)
| العنوان: | A second-generation anchored genetic linkage map of the tammar wallaby (Macropus eugenii) |
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| المؤلفون: | Wang, Chenwei, Webley, Lee, Wei, Ke-jun, Wakefield, Matthew J, Patel, Hardip R, Deakin, Janine E, Alsop, Amber, Marshall Graves, Jennifer A, Cooper, Desmond W, Nicholas, Frank W, Zenger, Kyall R |
| المصدر: | BMC Genetics |
| بيانات النشر: | BioMed Central |
| المجموعة: | Australian National University: ANU Digital Collections |
| مصطلحات موضوعية: | animals, chromosomes, artificial, bacterial, female, genetic markers, genotype, macropodidae, male, chromosome mapping |
| الوصف: | BACKGROUND The tammar wallaby, Macropus eugenii, a small kangaroo used for decades for studies of reproduction and metabolism, is the model Australian marsupial for genome sequencing and genetic investigations. The production of a more comprehensive cytogenetically-anchored genetic linkage map will significantly contribute to the deciphering of the tammar wallaby genome. It has great value as a resource to identify novel genes and for comparative studies, and is vital for the ongoing genome sequence assembly and gene ordering in this species. RESULTS A second-generation anchored tammar wallaby genetic linkage map has been constructed based on a total of 148 loci. The linkage map contains the original 64 loci included in the first-generation map, plus an additional 84 microsatellite loci that were chosen specifically to increase coverage and assist with the anchoring and orientation of linkage groups to chromosomes. These additional loci were derived from (a) sequenced BAC clones that had been previously mapped to tammar wallaby chromosomes by fluorescence in situ hybridization (FISH), (b) End sequence from BACs subsequently FISH-mapped to tammar wallaby chromosomes, and (c) tammar wallaby genes orthologous to opossum genes predicted to fill gaps in the tammar wallaby linkage map as well as three X-linked markers from a published study. Based on these 148 loci, eight linkage groups were formed. These linkage groups were assigned (via FISH-mapped markers) to all seven autosomes and the X chromosome. The sex-pooled map size is 1402.4 cM, which is estimated to provide 82.6% total coverage of the genome, with an average interval distance of 10.9 cM between adjacent markers. The overall ratio of female/male map length is 0.84, which is comparable to the ratio of 0.78 obtained for the first-generation map. CONCLUSIONS Construction of this second-generation genetic linkage map is a significant step towards complete coverage of the tammar wallaby genome and considerably extends that of the first-generation map. It will ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 1471-2156 |
| العلاقة: | http://hdl.handle.net/1885/95150Test; https://openresearch-repository.anu.edu.au/bitstream/1885/95150/4/01_Wang_A_second-generation_anchored_2011.pdf.jpgTest |
| DOI: | 10.1186/1471-2156-12-72 |
| الإتاحة: | https://doi.org/10.1186/1471-2156-12-72Test http://hdl.handle.net/1885/95150Test https://openresearch-repository.anu.edu.au/bitstream/1885/95150/4/01_Wang_A_second-generation_anchored_2011.pdf.jpgTest |
| حقوق: | © 2011 Wang et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0Test), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| رقم الانضمام: | edsbas.93EB08A |
| قاعدة البيانات: | BASE |
| تدمد: | 14712156 |
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| DOI: | 10.1186/1471-2156-12-72 |