دورية أكاديمية
Detection of alternative lengthening of telomeres by telomere quantitative PCR
| العنوان: | Detection of alternative lengthening of telomeres by telomere quantitative PCR |
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| المؤلفون: | Lau, LMS, Dagg, RA, Henson, JD, Au, AYM, Royds, JA, Reddel, RR |
| المصدر: | urn:ISSN:0305-1048 ; urn:ISSN:1362-4962 ; Nucleic Acids Research, 41, 2, e34-e34 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2013 |
| المجموعة: | UNSW Sydney (The University of New South Wales): UNSWorks |
| مصطلحات موضوعية: | Cancer, Genetics, Clinical Research, Biotechnology, Generic health relevance, Cell Line, Tumor, DNA, Neoplasm, Humans, Neoplasms, Oligonucleotide Probes, Polymerase Chain Reaction, Telomere, Telomere Homeostasis, anzsrc-for: 05 Environmental Sciences, anzsrc-for: 06 Biological Sciences, anzsrc-for: 08 Information and Computing Sciences |
| الوصف: | Alternative lengthening of telomeres (ALT) is one of the two known telomere length maintenance mechanisms that are essential for the unlimited proliferation potential of cancer cells. Existing methods for detecting ALT in tumors require substantial amounts of tumor material and are labor intensive, making it difficult to study prevalence and prognostic significance of ALT in large tumor cohorts. Here, we present a novel strategy utilizing telomere quantitative PCR to diagnose ALT. The protocol is more rapid than conventional methods and scrutinizes two distinct characteristics of ALT cells concurrently: long telomeres and the presence of C-circles (partially double-stranded circles of telomeric C-strand DNA). Requiring only 30 ng of genomic DNA, this protocol will facilitate large-scale studies of ALT in tumors and can be readily adopted by clinical laboratories. © 2012 The Author(s). |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | http://hdl.handle.net/1959.4/unsworks_75334Test; https://unsworks.unsw.edu.au/bitstreams/7d2bd10c-af5c-41b1-a7b4-f0c17bc7b0a5/downloadTest; https://doi.org/10.1093/nar/gks781Test |
| DOI: | 10.1093/nar/gks781 |
| الإتاحة: | https://doi.org/10.1093/nar/gks781Test http://hdl.handle.net/1959.4/unsworks_75334Test https://unsworks.unsw.edu.au/bitstreams/7d2bd10c-af5c-41b1-a7b4-f0c17bc7b0a5/downloadTest |
| حقوق: | open access ; https://purl.org/coar/access_right/c_abf2Test ; CC BY-NC ; https://creativecommons.org/licenses/by-nc/4.0Test/ ; free_to_read |
| رقم الانضمام: | edsbas.6D706ABD |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/nar/gks781 |
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