نتائج البحث - "Lactate dehydrogenase"

21

Clinical Characteristics of Adult Hemophagocytic Lymphohistiocytosis in the Emergency Department

المؤلفون: Xiangmin Li, Jia Li, Fangjie Zhang, Guo-Qing Huang, Aimin Wang, Ji Xu

المصدر: International Journal of General Medicine

الوصف: Fang-Jie Zhang, Guo-Qing Huang, Jia Li, Ji Xu, Xiang-Min Li, Ai-Min Wang Department of Emergency Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, Peopleâs Republic of ChinaCorrespondence: Xiang-Min Li; Ai-Min WangDepartment of Emergency Medicine, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan, 410008, Peopleâs Republic of ChinaTel +8613875858068; +8613875927399Email lxm8229@126.com; wangaimin@csu.edu.cnPurpose: To determine the clinical manifestations and results of adult hemophagocytic lymphohistiocytosis (HLH) patients in our emergency department.Methods: We retrospectively evaluated patients with HLH from 1 April 2018 to 31 December 2020. The clinical data of these patients (basic information, symptoms, vital signs, laboratory results, HLH diagnostic criteria, H Score, main treatments, outcomes) were collected.Results: Thirty-three patients (23 males and 10 females; 40.55Â± 18.78 years) with 34 clinical episodes (one male had two clinical episodes and died during the second episode) were enrolled. Twenty-five patients were placed in a âsurvivorâ group, and nine patients were categorized into a âdeceasedâ group. Fever, splenomegaly, hemoglobin < 90 g/L and platelet count < 100Ã 109/L most commonly met the diagnostic standard for HLH. The H Score results in the survival group and deceased group was 212.4Â± 37.18 and 252.1Â± 40.95, respectively. Viral infection was the most common reason for HLH, followed by immune-system disease and cancer. Laboratory tests showed that deceased-group patients had multiple-organ dysfunction. Multivariate logistic regression showed that the lactate dehydrogenase (lactate dehydrogenase) level (P = 0.039; odds ratio, 0.999) was significantly related to death.Conclusion: In the emergency department, HLH should be considered for critically ill patients with fever, splenomegaly, low hemoglobin and low platelet count. The H Score might be useful to diagnose HLH quickly. In our study, 26.47% of HLH patients died in the emergency department, and patients with a significantly increased lactate dehydrogenase level had a markedly increased risk of death.Keywords: clinical characteristics, hemophagocytic lymphohistiocytosis, emergency department, mortality, lactate dehydrogenase

وصف الملف: text/html

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e3174990543d2e11278e3312691c468Test
https://doi.org/10.2147/ijgm.s326270Test

22

Diagnostic Value of Lactate Dehydrogenase and Uric Acid as Screening Tools for Malignancies in Children

المؤلفون: Irene A, Oriaifo, James M, Gerard, Scott M, Thomas

المصدر: Pediatric Emergency Care. 38:e1327-e1331

مصطلحات موضوعية: L-Lactate Dehydrogenase, ROC Curve, Neoplasms, Pediatrics, Perinatology and Child Health, Emergency Medicine, Humans, General Medicine, Child, Biomarkers, Retrospective Studies, Uric Acid

الوصف: The aim of this study was to determine the diagnostic value of lactate dehydrogenase (LDH) and uric acid (UA) in children undergoing evaluation for possible malignancies.This was a retrospective chart review of patients aged 0 to 18 years presenting to an urban, tertiary care, pediatric hospital between July 1, 2011, and July 1, 2016. Patients were included if they had an LDH and/or UA level drawn, and they were excluded if they had a known cancer diagnosis. Sensitivity, specificity, and receiver operating characteristic curves were calculated for each biomarker.Six hundred five subjects were included in this study; 579 and 384 subjects had LDH and UA levels drawn, respectively; 15.7% had a final diagnosis of malignancy (49 leukemia, 46 nonleukemia).The specificities of both biomarkers for all types of malignancies were lower than their respective sensitivities. Comparing leukemic versus nonleukemic malignancies, the areas under the curve were 0.848 and 0.719, respectively, for LDH and 0.681 and 0.555, respectively, for UA.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::599f2c794ca08dfceab0b859e172ffd6Test
https://doi.org/10.1097/pec.0000000000002733Test

23

Analysis of the factors predicting clinical response to tocilizumab therapy in patients with severe COVID-19

المصدر: International Journal of Infectious Diseases
International Journal of Infectious Diseases, Vol 117, Iss, Pp 56-64 (2022)

مصطلحات موضوعية: Microbiology (medical), ALT, alanine transaminase, Infectious and parasitic diseases, RC109-216, clinical response, Antibodies, Monoclonal, Humanized, NAT, nucleic acid testing, SARS-CoV-2, severe acute respiratory syndrome coronavirus 2, Article, tocilizumab, RT-PCR, reverse transcription polymerase chain reaction, AST, aspartate transaminase, LPV/r, lopinavir/ritonavir, Humans, IL-6, interleukin-6, ARDS, acute respiratory distress syndrome, IQR, interquartile range, Retrospective Studies, COVID-19, coronavirus disease 2019, LDH, lactate dehydrogenase, SARS-CoV-2, TCZ, tocilizumab, COVID-19, HCQ, hydroxychloroquine, General Medicine, ICU, intensive care unit, COVID-19 Drug Treatment, early initiation, predictors, IMV, invasive mechanical ventilation, Infectious Diseases, CRP, C-reactive protein, IFN-β, interferon-β, OTR, oxygen therapy requirements, SCI, Significant clinical improvement, ePO2/FiO2, estimated arterial oxygen/fraction of inspired oxygen ratio

الوصف: Background: Controversy remains about the efficacy of tocilizumab (TCZ) for the treatment of severe COVID-19. We aimed to analyze the profile of TCZ-respondent patients. Methods: We retrospectively analyzed a cohort of patients with severe COVID-19 who received off-label TCZ after recommendation by a local committee and were admitted to the University Hospital “12 de Octubre” until May 2020. The primary end point was a significant clinical improvement (SCI) on day 14 after administration of TCZ. Factors independently related to SCI were analyzed by multivariate logistic regression models. Results: Of 428 (63.3%) patients treated with TCZ, 271 (63.3%) experienced SCI. After adjustment for factors related to unfavorable outcomes, TCZ administration within the first 48 hours from admission (odds ratio [OR]: 1.98, 95% confidence Interval [95% CI]: 1.1–3.55; P = 0.02) and ALT levels >100 UI/L at day 0 (OR: 3.28; 95% CI: 1.3–8.1; P = 0.01) were independently related to SCI. The rate of SCI significantly decreased according to the time of TCZ administration: 70.2% in the first 48 hours from admission, 58.5% on days 3-7, and 45.1% after day 7 (P = 0.03 and P = 0.001, respectively). Conclusion: TCZ improves the prognosis of patients with COVID-19 the most if treatment starts within the first 48 hours after admission.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::acc8c1e31738c5ea88d1147f0a289adfTest
https://doi.org/10.1016/j.ijid.2022.01.040Test

24

Inhibition of LncRNA-NEAT1 alleviates intestinal epithelial cells (IECs) dysfunction in ulcerative colitis by maintaining the homeostasis of the glucose metabolism through the miR-410-3p-LDHA axis

المؤلفون: Siyi, Ni, Yingchao, Liu, Jihong, Zhong, Yan, Shen

المصدر: Bioengineered. 13:8961-8971

مصطلحات موضوعية: Lipopolysaccharides, MicroRNAs, Glucose, Homeostasis, Humans, Colitis, Ulcerative, Epithelial Cells, RNA, Long Noncoding, Bioengineering, General Medicine, Lactate Dehydrogenase 5, Applied Microbiology and Biotechnology, Biotechnology

الوصف: Dysfunction of intestinal epithelial cells (IECs) leads to intestinal epithelial barrier damage and critically involves in the pathogenesis and development of ulcerative colitis (UC). Accumulating studies revealed essential functions of non-coding RNAs in UC. LncRNA NEAT1 (long non-coding RNA nuclear paraspeckle assembly transcript 1) is frequently dysregulated in diverse human diseases. Currently, the precise roles of NEAT1 in the dysfunction of IECs during UC remain unclear. We report NEAT1 was significantly upregulated in IECs from UC patients. In addition, microRNA-410-3p was remarkedly suppressed in IECs from UC patients. Silencing NEAT1 effectively ameliorates the LPS-induced IECs dysfunction. Bioinformatical analysis, RNA pull-down and luciferase assays illustrated that NEAT1 sponged miR-410-3p to downregulate its expression in IECs. Interestingly, the glucose metabolism was obviously elevated in IECs from UC compared with normal colon tissues. Furthermore, NEAT1 promoted and miR-410-3p suppressed glucose metabolism of IECs. We identified lactate dehydrogenase A (LDHA), a glucose metabolism key enzyme, was a direct target of miR-410-3p in IECs. Rescue experiments verified that restoration of miR-410-3p in NEAT1-overexpressing IECs successfully overcame the NEAT1-promoted cell death under LPS treatment by targeting LDHA. In summary, these results unveiled new roles and molecular mechanisms for the NEAT1-mediated IECs dysfunction during the ulcerative colitis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ce60b9f6cfb84ccfbe80b0451d1654cTest
https://doi.org/10.1080/21655979.2022.2037957Test

25

De Ritis‐adjusted AST provides comparable efficacy to lactate dehydrogenase as a biomarker for detection of LVAD hemolysis or thrombosis

المؤلفون: Sarah Schettle, Andrew Rosenbaum, Daniel Goodman, John Stulak

المصدر: Artificial Organs. 46:1425-1428

مصطلحات موضوعية: Biomaterials, L-Lactate Dehydrogenase, Biomedical Engineering, Humans, Medicine (miscellaneous), Thrombosis, Bioengineering, Heart-Assist Devices, General Medicine, Hemolysis, Biomarkers, Retrospective Studies

الوصف: Lactate hydrogenase (LDH) is a common biomarker utilized in the detection and monitoring of left ventricular assist device (LVAD) hemolysis and thrombosis. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are common laboratory tests that can be used to calculate the De Ritis ratio and the De Ritis adjusted AST.A retrospective review of LVAD patients was performed to identify three cohorts of patients: those with confirmed pump thrombosis after device exchange, those with LVAD-related hemolysis who were medically managed without pump exchange, and those who did not meet these criteria and served as the control cohort. Evaluation of AST, AST/ALT ratio (referred to as the De Ritis ratio) as well as AST x (AST/ALT) or the De Ritis-adjusted AST (DRA) was performed.There were 29 patients who underwent device exchange for thrombosis, 25 patients who were diagnosed with hemolysis and treated medically (clopidogrel (N = 6), heparin (N = 13), tirofiban (N = 8), eptifibatide (N = 2), and some received more than one of these treatments), and 425 control patients. A qualitatively comparable relative and absolute rise in DRA and LDH were found in both surgically managed pump thrombosis and suspected device-related hemolysis.Both AST and LDH as well DRA are significantly associated with pump thrombosis (p 0.0001 for each). DRA is a potential screening biomarker for hemolysis and device thrombosis in stable left ventricular assist device patients.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a5465d032d9d5d3284d084fd47b5937Test
https://doi.org/10.1111/aor.14236Test

26

The Clinical Risk Factors of Adenovirus Pneumonia in Children Based on the Logistic Regression Model: Correlation with Lactate Dehydrogenase

المؤلفون: Yuqiao Liu, Yang Shen, Botao Wei

المصدر: International Journal of Clinical Practice. 2022:1-6

مصطلحات موضوعية: Logistic Models, Article Subject, L-Lactate Dehydrogenase, ROC Curve, Risk Factors, Pneumonia, Viral, Pneumonia, Bacterial, Humans, General Medicine, Child, respiratory tract diseases, Adenoviridae, Retrospective Studies

الوصف: Background and Objective. Adenovirus pneumonia is a severe disease in children. Constructing a prognostic model contributes to individualized treatment of children with adenovirus pneumonia. Thus, a machine learning model was constructed in this study to explore the clinical and baseline characteristics of pneumonia and predict the type of pneumonia. Methods. Children with bacterial pneumonia (41 cases) and adenovirus pneumonia (179 cases) hospitalized in Tianjin Children’s Hospital from January to October 2020 were selected. The differences in baseline and clinical characteristics between children with two pneumonias, respectively, were compared via the chi-square test and Wilcox test. The Least Absolute Shrinkage and Selection Operator (LASSO) model was applied to screen the pneumonia type-related characteristics. Patients were randomly divided into the training set (n = 154) and test set (n = 66). The logistic model was constructed using the screened characteristics in the training set to predict whether the cases are bacterial pneumonia or adenovirus pneumonia. Finally, the model was validated by receiver operating characteristic (ROC) curve and area under curve (AUC) in the test set. Results. The age ( p < 0.001 ), hospital stay ( p < 0.001 ), tonsil condition ( p < 0.001 ), interleukin-6 (IL-6; p = 0.033 ), and lactate dehydrogenase (LDH; p < 0.001 ) between children with bacterial pneumonia and adenovirus pneumonia were significantly different. Sex, tonsil condition, age, hospital stay, r-glutamyltransferase (r-GT), and LDH levels were the factors associated with the types of pneumonia. Compared with bacterial pneumonia, children with adenovirus pneumonia were younger (OR = 0.207, 95% CI: 0.041–0.475), with longer hospital stay (OR = 7.974, 95% CI: 2.626–74.354) and higher LDH expression level (OR = 1.025, 95% CI: 1.006–1.060). 92.4% types of pneumonia were correctly predicted, and the AUC value of the model was 0.981. Conclusion. The LDH level was the associated factor to predict the types of pneumonia. Adenovirus pneumonia was associated with earlier age and longer hospital stay than bacterial pneumonia. The established model can well predict the types of pneumonia in children and provide clinical basis for guiding the individualized treatment of children.

وصف الملف: text/xhtml

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::239e46222744abe0b0153a67db8a9393Test
https://doi.org/10.1155/2022/3001013Test

27

Serum-Derived Exosomes-Mediated Circular RNA ARHGAP10 Modulates the Progression of Non-Small Cell Lung Cancer Through the miR-638/FAM83F Axis

المؤلفون: Jinbiao Xu, Zhanmin Zhang, Xuan Chen, Feng Qiu, Ke Fang, Huanwen Xiong

المصدر: Cancer Biotherapy and Radiopharmaceuticals. 37:96-110

الوصف: Background: Non-small-cell lung cancer (NSCLC) is the leading cause of cancer deaths all over the world. Exosomes exert central roles in intercellular communication. Circular RNA Rho GTPase activating protein 10 (circARHGAP10) was related to the development of NSCLC. Nevertheless, it was unclear whether circARHGAP10 can be mediated by serum-derived exosomes in NSCLC. Materials and Methods: Protein expression of CD63, CD81, family with sequence similarity 83F (FAM83F), glucose transporter 1 (Glut1), and lactate dehydrogenase were evaluated through western blot analysis. The expression of circARHGAP10, miR-638, and FAM83F was examined by quantitative real-time polymerase chain reaction. Cell proliferation, migration, and invasion were evaluated through 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) or transwell assays. Glucose consumption and lactate production were analyzed with special commercial kits. The relationship between circARHGAP10 or FAM83F and miR-638 was identified by dual-luciferase reporter or RNA immunoprecipitation (RIP) assays. The role of circARHGAP10 in vivo was confirmed through xenograft assay. Results: circARHGAP10 was upregulated in NSCLC tissues, cells, and serum-derived exosomes. Serum-derived exosomes boosted the expression of circARHGAP10 in NSCLC cells. circARHGAP10 depletion repressed proliferation, migration, invasion, and glycolysis of NSCLC cells in vitro, and curbed tumor growth in vivo. Also, miR-638 acted as a target of circARHGAP10, miR-638 overexpression overturned circARHGAP10 upregulation-mediated acceleration of proliferation, migration, invasion, and glycolysis of NSCLC cells. Besides, miR-638 targeted FAM83F and FAM83F overexpression abolished miR-638 enhancement-mediated proliferation, migration, invasion, and glycolysis of NSCLC cells. Conclusions: Inhibition of serum-derived exosomes-mediated circARHGAP10 curbed NSCLC progression through the miR-638/FAM83F axis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d868ea782a93af54b884335456dfba4Test
https://doi.org/10.1089/cbr.2019.3534Test

28

Impact of Individualized Hemolysis Management Based on Biological Variation Cut-offs in a Clinical Laboratory

المؤلفون: Fernando Marques-Garcia, David Hansoe Heredero Jung, Sandra Elena Pérez

المصدر: Annals of Laboratory Medicine. 42:169-177

الوصف: Background Hemolysis is the most common type of preanalytical interference. Cut-offs based on the hemolysis index level can be established using different approaches. The Working Group for Preanalytical Phase of the European Federation of Laboratory Medicine has developed a protocol for hemolysis management based on cut-offs estimated from biological variation (BV) and the use of interpretative comments. We developed and assessed the implementation of the protocol in our laboratory. Methods Hemolysates from whole blood were prepared following the Meites method, and pooled serum samples with known Hb concentrations were prepared. For each analyte (42 ), interferograms were generated and used to establish cut-offs: desirable analytical quality specification and reference change value. This protocol was assessed, both pre- and post-implementation, according to expert rules in the Laboratory Information System. Results Among the analytes evaluated, we selected those that showed the highest degree of hemolysis interference: lactate dehydrogenase (LDH), aspartate aminotransferase, direct bilirubin, potassium, and folic acid. The cut-offs for LDH and direct bilirubin were the lowest. Only 28.16% of all LDH values were adequately reported in the pre-implantation retrospective study, but this percentage improved in the post-implementation stage. Conclusions The development and implementation of a harmonized protocol for hemolysis management based on BV cut-offs and result reporting significantly improve hemolysis detection and lead to a decrease in the number of hemolyzed samples over time.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::348f9a6a76f804170e37b30f0d40d5ddTest
https://doi.org/10.3343/alm.2022.42.2.169Test

29

Clinical Significance and Prognostic Value of Lactate Dehydrogenase Expression in Cervical Cancer

المؤلفون: Yuping Ye, Min Chen, Xinyan Chen, Jingyu Xiao, Lin Liao, Faquan Lin

المصدر: Genet Test Mol Biomarkers

مصطلحات موضوعية: L-Lactate Dehydrogenase, Biomarkers, Tumor, Humans, Uterine Cervical Neoplasms, Female, Kaplan-Meier Estimate, Original Articles, General Medicine, Prognosis, Genetics (clinical)

الوصف: BACKGROUND: Lactate dehydrogenase (LDH) is a marker of injury and disease as it is expressed extensively in numerous cell types and tissues. Moreover it is released during tissue breakdown, and is elevated in cancerous tissues. However, the clinical significance and prognostic value of LDH as a tumor marker have been subject to considerable discussion. OBJECTIVE: In this study, clinical serum LDH data from patients with cervical cancer (CC), CC microarray data, and RNA-seq data were integrated to assess the expression of LDH in CC. METHODS: A total of 204 patients with newly diagnosed CC and 204 age-matched healthy controls were included to evaluate serum LDH levels in CC and non-cancer samples. External microarrays and RNA-seq datasets were collected for the differential expression analysis of LDH in CC and non-cancer tissue samples. Kaplan-Meier survival curves of the prognostic value of LDH for CC were plotted for RNA-seq data. Functional enrichment analysis was performed for the genes co-expressed with LDH. RESULTS: The data from our in-house clinical cases as well as the data extracted from microarrays and RNA-seq databases demonstrated significant overexpression of LDH in CC samples. Elevated LDH expression levels were associated with poor overall survival in CC patients. The genes co-expressed with LDH were significantly correlated with the biological processes and pathways, associated with nuclear division, the condensed chromosome, protein serine/threonine kinase activity, and the cell cycle. CONCLUSION: In conclusion, LDH upregulation might serve as a therapeutic and prognostic biomarker for CC.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::759d733b4eff4ce55c9bea267ab7bcd7Test
https://doi.org/10.1089/gtmb.2021.0006Test

30

Protective effect of liquiritin on coronary heart disease through regulating the proliferation of human vascular smooth muscle cells via upregulation of sirtuin1

المؤلفون: Liang Yuan, Dajie Wang, Chunyang Wu

المصدر: Bioengineered, Vol 13, Iss 2, Pp 2840-2850 (2022)

الوصف: This study aimed to explore whether liquiritin affects the development of coronary heart disease by regulating the proliferation and migration of human vascular smooth muscle cells (hVSMCs). A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2 H-tetrazolium bromide (MTT) assay and lactate dehydrogenase (LDH) release detection were performed to measure the toxic effects of liquiritin on hVSMCs. An in vitro atherosclerosis model in hVSMCs was established using oxidized low-density lipoprotein (ox-LDL), and cell proliferation and apoptosis were detected using an MTT assay and flow cytometry analysis. Western blotting and reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) were used to detect protein and mRNA expressions, respectively. Caspase3 activity and cell migration were measured using an activity detection kit and Transwell assay, respectively. The results indicated that liquiritin at doses

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17d80677f95a03a4962386a601418a0fTest
https://doaj.org/article/05762d0021dc438aa0d8b22d72b30a29Test

تنقيح النتائج