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المؤلفون: Marta Fridrichová, Katarina Pomorska, Vladislav Jakubu, Katerina Neradova, Helena Zemlickova
المصدر: Folia Microbiologica
مصطلحات موضوعية: Adult, Male, Methicillin-Resistant Staphylococcus aureus, Czech, medicine.medical_specialty, Adolescent, Genotype, Short Communication, Bacteremia, Context (language use), Microbial Sensitivity Tests, Biology, medicine.disease_cause, Microbiology, Hospitals, University, Young Adult, 03 medical and health sciences, Epidemiology, medicine, Humans, Typing, Child, Aged, Czech Republic, 030304 developmental biology, Aged, 80 and over, 0303 health sciences, 030306 microbiology, Infant, General Medicine, Middle Aged, Staphylococcal Infections, Methicillin-resistant Staphylococcus aureus, language.human_language, Anti-Bacterial Agents, Bacterial Typing Techniques, Phenotype, Staphylococcus aureus, Child, Preschool, language, Multilocus sequence typing, Female, Multilocus Sequence Typing
الوصف: The aim of this study was to trace the dynamic changes of methicillin-resistant Staphylococcus aureus (MRSA) lineages in the local hospital in both the national and international context. We describe genotypic and phenotypic characterization of 62 non-duplicate MRSA isolates collected during 2010–2016 at University Hospital in Hradec Kralove, Czech Republic. The isolates were characterized by multilocus sequence typing (MLST), spa typing, and staphylococcal cassette chromosome mec typing (SCCmec typing). Eight different genotypes were described; ST225-t003-II (32/62, 52%), ST5-t002-II (13/62, 22%), and ST225-t014-II (12/62, 21%) were constantly detected over the 7-year follow-up period. The genotypes ST225-t151-II, ST225-t1282-II, ST225-t1623-II, ST78-t2832-II, and ST225-t8799-II occurred only once in the period reported. The majority of the strains, represented by ST225, belonged to clonal complex 5 (CC5).
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5dea37e476f2f81657fb98e9e86eedf5Test
https://doi.org/10.1007/s12223-020-00782-9Test -
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المؤلفون: Martina Kolackova, Ivana Musilova, Marcela Drahosova, Petr Matlak, Helena Zemlickova, Ctirad Andrys, Romana Gerychová, Bo Jacobsson, Petr Janku, Marian Kacerovsky, Lenka Pliskova, Ondrej Simetka, Barbora Zednikova
المصدر: Fetal Diagnosis and Therapy. 46:402-410
مصطلحات موضوعية: Fetal Membranes, Premature Rupture, Embryology, medicine.medical_specialty, Amniotic fluid, Enzyme-Linked Immunosorbent Assay, Cervix Uteri, 03 medical and health sciences, 0302 clinical medicine, Predictive Value of Tests, Pregnancy, medicine, Humans, Rupture of membranes, Radiology, Nuclear Medicine and imaging, 030212 general & internal medicine, Retrospective Studies, Pentraxin-3, 030219 obstetrics & reproductive medicine, medicine.diagnostic_test, Obstetrics, business.industry, Obstetrics and Gynecology, General Medicine, Amniotic Fluid, Up-Regulation, 3. Good health, Serum Amyloid P-Component, C-Reactive Protein, Chorioamnionitis, Pediatrics, Perinatology and Child Health, Amniocentesis, Female, Amniotic cavity, business, Biomarkers
الوصف: Problem: To determine the changes of pentraxin 3 (PTX3) level in noninvasively obtained cervical fluid samples from women with preterm prelabor rupture of membranes (PPROM) based on the presence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI), and intra-amniotic infection (the presence of both MIAC and IAI). Methods of Study: A total of 160 women with PPROM were included. Cervical fluid samples were obtained using a Dacron polyester swab and amniotic fluid samples were obtained by transabdominal amniocentesis. Cervical fluid PTX3 levels were assessed using enzyme-linked immunosorbent assay. Results: PTX3 was found in all the cervical fluid samples and its levels were higher in women with MIAC, IAI, and intra-amniotic infection than in women without these conditions. When the women were categorized into four subgroups based on the presence of MIAC and/or IAI, women with intra-amniotic infection had higher cervical fluid PTX3 levels than those with sterile IAI (IAI alone), colonization (MIAC alone), or no MIAC or IAI. A cervical fluid PTX3 level of 11 ng/mL was the best value for identifying the presence of intra-amniotic infection in women with PPROM. Conclusions: PTX3 is a constituent of cervical fluid of women with PPROM. Cervical fluid PTX3 level reflects the situation in the intra-amniotic compartments of women with PPROM. Cervical fluid PTX3 is a potential marker for the noninvasive identification of intra-amniotic infection in PPROM.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3630f146833121a696fce29909a51ce3Test
https://doi.org/10.1159/000499482Test -
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المصدر: BMC Veterinary Research
BMC Veterinary Research, Vol 16, Iss 1, Pp 1-6 (2020)مصطلحات موضوعية: Adult, Methicillin-Resistant Staphylococcus aureus, Czech, Veterinary medicine, Livestock, Genotype, Livestock-associated MRSA, Nose, medicine.disease_cause, Veterinarians, 03 medical and health sciences, Occupational Exposure, Prevalence, Animals, Humans, Medicine, Nasal carriage, Colonization, Students, Czech Republic, Aged, 030304 developmental biology, Carriage, 0303 health sciences, lcsh:Veterinary medicine, General Veterinary, 030306 microbiology, business.industry, MRSA colonization, General Medicine, Companion animals, Middle Aged, Staphylococcal Infections, Methicillin-resistant Staphylococcus aureus, language.human_language, Staphylococcus aureus, Carrier State, language, lcsh:SF600-1100, Education, Veterinary, business, Research Article
الوصف: Background Cases of colonization or infection caused by Methicillin-resistant Staphylococcus aureus (MRSA) are frequently reported in people who work with animals, including veterinary personnel. The aim of this study was to determine the prevalence of MRSA colonization among veterinary professionals. A total of 134 nasal swabs from healthy attendees of a veterinary conference held in the Czech Republic were tested for presence of MRSA. The stains were further genotypically and phenotypically characterized. Results Nine isolated MRSA strains were characterized with sequence type (ST), spa type (t) and Staphylococcal Cassette Chromosome mec type. Five different genotypes were described, including ST398-t011-IV (n = 5), ST398-t2330-IV (n = 1), ST398-t034-V (n = 1), ST225-t003-II (n = 1) and ST4894-t011-IV (n = 1). The carriage of the animal MRSA strain was confirmed in 8 cases, characteristics of one strain corresponded to the possible nosocomial origin. Among animal strains were described three spa types (t011, t034, t2330) belonging into one dominating clonal complex spa-CC11. Conclusion According to our results, the prevalence of nasal carriage of MRSA in veterinary personnel is 6.72%. Although we described an increase compared to the results of previous study (year 2008), the prevalence in the Czech Republic is still remaining lower than reported from neighboring countries. Our results also indicate that healthcare - associated MRSA strains are still not spread among animals.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae6047b9f6e688c054bc4cd7d6b10799Test
https://doi.org/10.1186/s12917-019-2223-zTest -
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المؤلفون: Costas C. Papagiannitsis, Katerina Chudejova, Jaroslav Hrabak, Lucie Kraftova, Anna Sramkova, Veronika Paskova, Efthimia Petinaki, Katerina Neradova, Vladislav Jakubu, Helena Zemlickova
المصدر: Folia microbiologica. 65(5)
مصطلحات موضوعية: Methicillin-Resistant Staphylococcus aureus, Bacterial Toxins, medicine.disease_cause, Microbiology, Sensitivity and Specificity, 03 medical and health sciences, medicine, Humans, Diagnostic Errors, 030304 developmental biology, 0303 health sciences, Reproducibility, 030306 microbiology, business.industry, Diagnostic Tests, Routine, SCCmec, Significant difference, Routine laboratory, Reproducibility of Results, General Medicine, Repeatability, biochemical phenomena, metabolism, and nutrition, Staphylococcal Infections, Rapid identification, Matrix-assisted laser desorption/ionization, Molecular Diagnostic Techniques, Staphylococcus aureus, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, business
الوصف: Rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) is essential for proper initial antibiotic therapy and timely set up of hygienic measures. Recently, detection of MRSA using MALDI-TOF mass spectrometer mediated by the peptide-phenol-soluble modulin (PSM-mec)-linked to the class A mec gene complex present in SCCmec cassettes types II, III, and VIII of MRSA strains, has been commercially available. We present here a multicentre study on MALDI-TOF MS detection of MRSA evincing a poor repeatability and reproducibility of the assay. The sensitivity of the assay varies between 50 and 90% in strains carrying psmMEC and psmδ genes encoding for PSM-mec and δ-toxin (a member of the PSM peptide family), respectively. No false positive results were found. The very major error calculation was 30% and the major error achieved 0%. Interlaboratory repeatability varies between 0 and 100%. No significant difference was observed with the use of different cultivation media. Our data showed a poor sensitivity of the method excluding it from the use in routine laboratory testing.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca67f403cc740aac5c5e5d07b354426bTest
https://pubmed.ncbi.nlm.nih.gov/32613406Test -
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المؤلفون: Céline Pulcini, Simone Mohrs, Bojana Beovic, Inge Gyssens, Ursula Theuretzbacher, Otto Cars, Golubinka Bosevska, Marcel Bruch, Karen Bush, Lidija Cizmovic, Nick Daneman, Béatrice Demoré, Aleksander Deptuła, Uga Dumpis, Aoife Fleming, Niels Frimodt-Mǿller, Helen Giamarellou, Ljiljana Gojkovic-Bukarica, Thorolfur Gudnason, Hakan Hanberger, Stephan Harbarth, Arjan Harxhi, Todor Kantardjiev, Doubravka Kostalova, Vladimir Krcmery, Katrin Kurvits, Endre Ludwig, Outi Lyytikäinen, Alasdair MacGowan, Síle O'Connor, Leonardo Pagani, George L. Petrikkos, Gabriel Adrian Popescu, Lul Raka, José Ramón Paño Pardo, Liliana Cristina Ramos Dias, Hege Salvesen-Blix, Gunnar Skov Simonsen, Evelina Tacconelli, John Turnidge, Rolanda Valintėlienė, Vera Vlahović-Palčevski, Peter Zarb, Helena Zemlickova
المساهمون: Service des Maladies Infectieuses et Tropicales [CHRU Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), ReAct-Action on Antibiotic Resistance, Department of Medical Sciences, Uppsala University, Uppsala, Sweden., University Medical Centre Ljubljana [Ljubljana, Slovenia] (UMCL), Department of Medicine, Radboud University Nijmegen Medical Centre, and Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands, Hasselt University, Hasselt, Belgium., Center for Anti-Infective Agents, Vienna, Austria., ESCMID Study Group for Antibiotic Policies (ESGAP), ReAct Working Group on Old Antibiotics
المصدر: International Journal of Antimicrobial Agents, 49, 98-101
International Journal of Antimicrobial Agents
International Journal of Antimicrobial Agents, Elsevier, 2017, 49 (1), pp.98-101. ⟨10.1016/j.ijantimicag.2016.09.029⟩
International Journal of Antimicrobial Agents, 49, 1, pp. 98-101مصطلحات موضوعية: 0301 basic medicine, Microbiology (medical), Canada, Drug marketing, medicine.drug_class, 030106 microbiology, Antibiotics, lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4], 03 medical and health sciences, 0302 clinical medicine, Antibiotic resistance, Systemic antibiotics, Drug resistance in microorganisms, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Surveys and Questionnaires, Environmental health, Drug approval, Humans, Medicine, Pharmacology (medical), 030212 general & internal medicine, ComputingMilieux_MISCELLANEOUS, business.industry, Drugs -- Marketing, Australia, Environmental ethics, General Medicine, United States, Anti-Bacterial Agents, 3. Good health, Europe, Resistant bacteria, Infectious Diseases, Equipment and Supplies, Scale (social sciences), Antibiotic Stewardship, business
الوصف: The objective of this study was to update a 2011 survey, conducted on behalf of the ESCMID Study Group for Antibiotic Policies (ESGAP), studying the availability of old but clinically useful antibiotics in North America, Europe and Australia. This follow-up survey was performed in 2015 in 40 countries among specialists from the pharmaceutical, infectious diseases and microbiology sectors in North America, Europe and Australia in order to assess the availability through usual marketing processes of 36 systemic antibiotics (addition of 3 antibiotics compared with the 2011 survey) selected for their ability to treat infections caused by resistant bacteria and their unique value for specific criteria. The questionnaire was sent by e-mail to national contacts belonging to ESGAP and ReAct networks. In all, 39 of the 40 countries participated in this survey. The number of available antibiotics differed considerably from one drug to another as well as from one country to another (e.g. 7 antibiotics available in Estonia, 24 in France). Overall, 25/36 selected antibiotics were marketed in 20/39 countries or less. From 2011 to 2015 (data available for both periods in 37 countries for 33 antibiotics), the number of available selected antibiotics increased in 13 countries and decreased in 17. In conclusion, despite the ongoing bacterial resistance crisis, the situation regarding the availability of ‘forgotten antibiotics’ has worsened since 2011. Urgent measures are needed to ensure better availability of these antibiotics on a global scale as a conservation measure to ensure sustainable and responsible use of antibiotics.
peer-reviewedوصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a3f9aab2db27835f962b22c75494832Test
https://doi.org/10.1016/j.ijantimicag.2016.09.029Test -
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المؤلفون: Michal Svarc, Lenka Ryskova, Miroslav Fajfr, Pavla Paterová, Rudolf Kukla, Lucia Mališová, Helena Zemlickova, Radka Bolehovska
المصدر: Folia Microbiologica
مصطلحات موضوعية: Male, Lung Neoplasms, Bordetella, Short Communication, Respiratory System, Respiratory tract, Microbiology, Tazobactam, 03 medical and health sciences, 0302 clinical medicine, Fatal Outcome, RNA, Ribosomal, 16S, Case report, medicine, Humans, MALDI-TOF MS, Clinical significance, 030212 general & internal medicine, Aged, Bordetella Infections, 0303 health sciences, Lung, biology, 030306 microbiology, business.industry, Septic shock, General Medicine, Bordetella trematum, medicine.disease, biology.organism_classification, Anti-Bacterial Agents, 16S rRNA sequencing, medicine.anatomical_structure, Respiratory failure, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, business, Piperacillin, medicine.drug
الوصف: We report the case of isolation ofBordetella trematumfrom the respiratory tract of a patient with lung carcinoma. This gram-negative, opportunistic rod was firstly described in 1996. To date, only several strains ofBordetella trematumhave been isolated and reported, mostly from skin and soft tissue infections. The patient was admitted to the ICU of the Pulmonary Department in incipient septic shock with respiratory failure. Intravenous fluid resuscitation and non-invasive ventilation were administered immediately. A broad spectrum antibiotic piperacillin/tazobactam was administered empirically after sampling of material for microbiological examination. The bronchoscopy showed a large cavern of decayed tumour invading into mediastinum. Both sample cultures showed significant quantities of gram-negative non-fermenting bacteria. The isolate was identified using MALDI-TOF MS asBordetella trematumand the identification was confirmed using 16S ribosomal RNA sequencing. In the last few years, routine bacterial identification using MALDI-TOF MS has enabled correct discrimination of this species. Nevertheless, isolation ofBordetella trematumin clinical samples is still very uncommon, and it is appropriate to confirm the species identification via 16S ribosomal RNA sequencing. To our knowledge, this is the first case ofB. trematumisolated from the human respiratory tract since its first description. The clinical significance ofBordetella trematumin the rapid deterioration of the patient’s status remains unclear.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d11c6a1c4660a9be28cd62e47cec760Test
https://pubmed.ncbi.nlm.nih.gov/32189223Test -
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المؤلفون: Katerina Chudejova, Jaroslav Hrabak, Matej Medvecky, Veronika Paskova, Roman Jirsa, Vladislav Jakubu, Helena Zemlickova, Costas C. Papagiannitsis, Ibrahim Bitar
المصدر: Diagnostic microbiology and infectious disease. 91(2)
مصطلحات موضوعية: 0301 basic medicine, Microbiology (medical), Transposable element, DNA, Bacterial, 030106 microbiology, Microbial Sensitivity Tests, Biology, Integron, beta-Lactam Resistance, beta-Lactamases, Integrons, Transposition (music), 03 medical and health sciences, Plasmid, Bacterial Proteins, Enterobacter cloacae, Humans, Sequence (medicine), Genetics, ColE1, Enterobacteriaceae Infections, General Medicine, biology.organism_classification, Enterobacteriaceae, Anti-Bacterial Agents, Infectious Diseases, biology.protein, Plasmids
الوصف: A VIM-1-producing ST92 Enterobacter cloacae was isolated in a Czech hospital. blaVIM-1 was part of the class 1 integron In110 carried by a Tn1721-like transposon. Tn1721-like was located on a ColE1-like plasmid, pEncl-30969cz (33,003 bp). Target site duplications at the boundaries of Tn1721-like suggested its transposition into the pEncl-30969cz backbone.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f4f6c4be2ff7d04e675c95861d89832Test
https://pubmed.ncbi.nlm.nih.gov/29477275Test -
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المؤلفون: Vaclava Adamkova, Martin Musilek, Helena Zemlickova, Vladislav Jakubu, Jitka Motlová, Pavla Urbášková
المصدر: Journal of Medical Microbiology. 59:1079-1083
مصطلحات موضوعية: Microbiology (medical), clone (Java method), Serotype, Biology, medicine.disease_cause, Microbiology, Pneumococcal Infections, Streptococcus pneumoniae, Odds Ratio, medicine, Humans, Serotyping, Czech Republic, Invasive disease, Infant, Newborn, Infant, General Medicine, Odds ratio, Streptococcaceae, biology.organism_classification, Virology, Carriage, Child, Preschool, Carrier State, Multilocus sequence typing
الوصف: To estimate the invasive disease potential of Streptococcus pneumoniae serotypes, invasive isolates (n=138) were compared with nasopharyngeal isolates (n=153) from children under 6 years of age in the Czech Republic. Odds ratios (ORs) based on a comparison of the distribution of S. pneumoniae serotypes amongst invasive and carriage isolates were calculated for individual serotypes and 172 strains were characterized using multilocus sequence typing. The ORs of serotypes 9V and 14 were significantly greater than 1, suggesting an association with invasive disease, while serotypes 6A and 23F were significantly associated with carriage (ORs less than 1). A single predominant clone with high invasive disease potential was found in each of the 9V, 7F, 14 and 1 serotypes while carriage-associated serotypes were highly heterogeneous.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce4c29b7da40ead17af395340acdae94Test
https://doi.org/10.1099/jmm.0.018390-0Test -
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المؤلفون: Celine Pulcini, Simone Mohrs, Bojana Beovic, Inge Gyssens, Ursula Theuretzbacher, Otto Cars, Golubinka Bosevska, Marcel Bruch, Karen Bush, Lidija Cizmovic, Nick Daneman, Béatrice Demoré, Aleksander Deptuła, Uga Dumpis, Aoife Fleming, Niels Frimodt-Mǿller, Helen Giamarellou, Ljiljana Gojkovic-Bukarica, Thorolfur Gudnason, Hakan Hanberger, Stephan Harbarth, Arjan Harxhi, Todor Kantardjiev, Doubravka Kostalova, Vladimir Krcmery, Katrin Kurvits, Endre Ludwig, Outi Lyytikäinen, Alasdair MacGowan, Síle O'Connor, Leonardo Pagani, George L. Petrikkos, Gabriel Adrian Popescu, Céline Pulcini, Lul Raka, José Ramón Pano Pardo, Liliana Cristina Ramos Dias, Hege Salvesen-Blix, Gunnar Skov Simonsen, Evelina Tacconelli, John Turnidge, Rolanda Valintėlienė, Vera Vlahović-Palčevski, Peter Zarb, Helena Zemlickova
المساهمون: Service des Maladies Infectieuses et Tropicales [CHRU Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), ReAct-Action on Antibiotic Resistance, Department of Medical Sciences, Uppsala University, Uppsala, Sweden., University Medical Centre Ljubljana [Ljubljana, Slovenia] (UMCL), Department of Medicine, Radboud University Nijmegen Medical Centre, and Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands, Hasselt University, Hasselt, Belgium., Centre for Anti-Infective Agents, Vienna, Austria.
المصدر: International Journal of Antimicrobial Agents
International Journal of Antimicrobial Agents, Elsevier, 2017, 49 (4), pp.482. ⟨10.1016/j.ijantimicag.2017.02.006⟩مصطلحات موضوعية: Microbiology (medical), 0303 health sciences, medicine.medical_specialty, 030306 microbiology, business.industry, medicine.drug_class, Published Erratum, Antibiotics, MEDLINE, General Medicine, Antimicrobial, 3. Good health, 03 medical and health sciences, 0302 clinical medicine, Infectious Diseases, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Environmental protection, Family medicine, Medicine, Pharmacology (medical), 030212 general & internal medicine, business, ComputingMilieux_MISCELLANEOUS
الوصف: International audience
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::290d8163f20307e28ad1fca32da4baddTest
https://doi.org/10.1016/j.ijantimicag.2017.02.006Test -
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المصدر: Folia microbiologica. 60(1)
مصطلحات موضوعية: Chromatography, Citrobacter freundii Complex, Analytical chemistry, General Medicine, Biology, Mass spectrometry, MALDI-TOF Mass Spectrometry, biology.organism_classification, Microbiology, Biochemistry, Citrobacter freundii, Bacterial Typing Techniques, Matrix (chemical analysis), Citrobacter, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Genus Citrobacter, Species identification, Typing
الوصف: Strains of genus Citrobacter (152 isolates from 1950 to 1988 deposited in the Czech National Collection of Type Cultures, Prague) were re-classified using biological and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) methods. One-hundred thirty-six strains (ca. 90 %) were identified to the species level using the biological method with evaluation by Farmer matrix. MALDI-TOF MS exhibited better identification capability, the data being more compact; the method was unambiguously successful in typing 145 (95 %) strains. Comparison of the results of identification by the two methods revealed differences (for 12 samples) in identified species which, considering all biochemical and/or MS characteristics, could be attributed to the natural variability of strains and close relation of the misidentified species (all of them belonged to the Citrobacter freundii complex). Taking into account all the above data, both methods can be considered reliable; however, the MALDI-TOF MS exhibits higher accuracy, efficiency, and rapidity.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e5b27538b095c6bcc5ecd482acc1c60Test
https://pubmed.ncbi.nlm.nih.gov/25113540Test
