miR‐124‐3p availability is antagonized by LncRNA‐MALAT1 for Slug‐induced tumor metastasis in hepatocellular carcinoma
| العنوان: | miR‐124‐3p availability is antagonized by LncRNA‐MALAT1 for Slug‐induced tumor metastasis in hepatocellular carcinoma |
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| المؤلفون: | Xiaoguang Yu, Zheng-Yuan Jiang, Wei Wang, Chi Liu, Xiulan Zheng, Rongjun Cui, Yujia Pan, Jialin Fan, Jiebing Tang, Jia-Hui Wan, Yu-Cui Lin |
| المصدر: | Cancer Medicine, Vol 8, Iss 14, Pp 6358-6369 (2019) Cancer Medicine |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Cancer Research, Metastasis, Mice, miR‐124‐3p, 0302 clinical medicine, Neoplasm Metastasis, Original Research, Cancer Biology, MALAT1, biology, Liver Neoplasms, Cell migration, hepatocellular carcinoma, Middle Aged, Prognosis, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Immunohistochemistry, Gene Expression Regulation, Neoplastic, Oncology, 030220 oncology & carcinogenesis, Gene Knockdown Techniques, Adenocarcinoma, Female, RNA Interference, RNA, Long Noncoding, Adult, metastasis‐associated lung adenocarcinoma transcript 1, Carcinoma, Hepatocellular, Slug, lcsh:RC254-282, 03 medical and health sciences, Cell Line, Tumor, medicine, Animals, Humans, Radiology, Nuclear Medicine and imaging, Aged, Neoplasm Staging, tumor metastasis, Oncogene, fungi, biology.organism_classification, medicine.disease, Xenograft Model Antitumor Assays, digestive system diseases, SNAI2, Disease Models, Animal, MicroRNAs, 030104 developmental biology, ROC Curve, Tumor progression, Cancer research, Snail Family Transcription Factors |
| الوصف: | Background As an oncogene, long noncoding RNA metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1) can promote tumor metastasis. Hyperexpression of MALAT1 has been observed in many malignant tumors, including hepatocellular carcinoma (HCC). However, the role and mechanism of MALAT1 in HCC remain unclear. Methods Thirty human HCC and paracancerous tissue specimens were collected, and the human hepatoma cell lines Huh7 and HepG2 were cultured according to standard methods. MALAT1 and Snail family zinc finger (Slug) expression were measured by real‐time PCR, immunohistochemistry, and western blotting. Luciferase reporter assay and RNA immunoprecipitation (RIP) assay verified the direct interaction between miR‐124‐3p and Slug(SNAI2) or MALAT1. Wound healing and transwell assays were performed to examine invasion and migration, and a subcutaneous tumor model was established to measure tumor progression in vivo. Results MALAT1 expression was upregulated in HCC tissues and positively correlated with Slug expression. MALAT1 and miR‐124‐3p bind directly and reversibly to each other. MALAT1 silencing inhibited cell migration and invasion. miR‐124‐3p inhibited HCC metastasis by targeting Slug. Conclusions MALAT1 regulates Slug through miR‐124‐3p, affecting HCC cell metastasis. Thus, the MALAT1/miR‐124‐3p/Slug axis plays an important role in HCC. Metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1) regulates Slug through miR‐124‐3p, affecting cell migration and cell invasion. Thus, the MALAT1/miR‐124‐3p/Slug axis plays an important role in hepatocellular carcinoma. |
| اللغة: | English |
| تدمد: | 2045-7634 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::228eb2a64539a1b319c810563acd63f6Test https://doaj.org/article/e4d3de2f7014489ea93ade62a7e7cc0dTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....228eb2a64539a1b319c810563acd63f6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20457634 |
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