المؤلفون: Garcia, Elizabeth Andrea, Blanco, Federico Carlos, Bigi, María Mercedes, Vazquez, Cristina Lourdes, Forrellad, Marina Andrea, Rocha, Rosana Valeria, Golby, Paul, Soria, Marcelo Abel, Bigi, Fabiana

المصدر: Veterinary microbiology 222 : 30-38. (August 2018)

مصطلحات موضوعية: Mycobacterium Bovis, Expresión Génica, Components, Gene Expression, PhoPR

الوصف: Mycobacterium bovis is the causative agent of bovine tuberculosis and is a member of Mycobacterium tuberculosis complex, which causes tuberculosis in a number of mammals including humans. Previous studies have shown that the genes encoding the two-component system PhoPR, which regulates several genes involved in the virulence of M. tuberculosis, are polymorphic in M. bovis, when compared to M. tuberculosis, which results in a dysfunctional two-component system. In this study we investigated the role of PhoPR in two M. bovis strains with differing degrees of virulence. We found that the deletion of phoP in an M. bovis isolate reduced its capacity of inducing phagosomal arrest in bovine macrophages. By gene expression analysis, we demonstrated that, in both M. bovis strains, PhoP regulates the expression of a putative lipid desaturase Mb1404-Mb1405, a protein involved in redox stress AhpC, the sulfolipid transporter Mmpl8 and the secreted antigen ESAT-6. Furthermore, the lack of PhoP increased the sensitivity to acidic stress and alteration of the biofilm/pellicle formation of M. bovis. Both these phenotypes are connected to bacterial redox homeostasis. Therefore, the results of this study suggest a role of PhoPR in M. bovis to be linked to the mechanisms that mycobacteria display to maintain their redox balance. ; Instituto de Biotecnología ; Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía, Cátedra de Microbiología Agrícola; Argentina. . Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de ...

وصف الملف: application/pdf

العلاقة: http://hdl.handle.net/20.500.12123/4531Test; https://www.sciencedirect.com/science/article/pii/S0378113518300725?via%3DihubTest; https://doi.org/10.1016/j.vetmic.2018.06.016Test

الإتاحة: https://doi.org/20.500.12123/4531Test
https://doi.org/10.1016/j.vetmic.2018.06.016Test
https://hdl.handle.net/20.500.12123/4531Test
https://www.sciencedirect.com/science/article/pii/S0378113518300725?via%3DihubTest

المؤلفون: Garcia, Elizabeth Andrea, Blanco, Federico Carlos, Pujadas Bigi, María Montserrat, Vázquez, Cristina Lourdes, Forrellad, Marina Andrea, Rocha, Rosana Valeria, Golby, Paul, Soria, Marcelo Abel, Bigi, Fabiana

مصطلحات موضوعية: GENE EXPRESSION, MYCOBACTERIUM BOVIS, PHOPR, TWO-COMPONENT SYSTEM, https://purl.org/becyt/ford/1.6Test, https://purl.org/becyt/ford/1Test

الوصف: Mycobacterium bovis is the causative agent of bovine tuberculosis and is a member of Mycobacterium tuberculosis complex, which causes tuberculosis in a number of mammals including humans. Previous studies have shown that the genes encoding the two-component system PhoPR, which regulates several genes involved in the virulence of M. tuberculosis, are polymorphic in M. bovis, when compared to M. tuberculosis, which results in a dysfunctional two-component system. In this study we investigated the role of PhoPR in two M. bovis strains with differing degrees of virulence. We found that the deletion of phoP in an M. bovis isolate reduced its capacity of inducing phagosomal arrest in bovine macrophages. By gene expression analysis, we demonstrated that, in both M. bovis strains, PhoP regulates the expression of a putative lipid desaturase Mb1404-Mb1405, a protein involved in redox stress AhpC, the sulfolipid transporter Mmpl8 and the secreted antigen ESAT-6. Furthermore, the lack of PhoP increased the sensitivity to acidic stress and alteration of the biofilm/pellicle formation of M. bovis. Both these phenotypes are connected to bacterial redox homeostasis. Therefore, the results of this study suggest a role of PhoPR in M. bovis to be linked to the mechanisms that mycobacteria display to maintain their redox balance. ; Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina ; Fil: Pujadas Bigi, María Montserrat. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina ; Fil: Vázquez, Cristina ...

وصف الملف: application/pdf

العلاقة: info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378113518300725Test; http://hdl.handle.net/11336/117550Test; Garcia, Elizabeth Andrea; Blanco, Federico Carlos; Pujadas Bigi, María Montserrat; Vázquez, Cristina Lourdes; Forrellad, Marina Andrea; et al.; Characterization of the two component regulatory system PhoPR in Mycobacterium bovis; Elsevier Science; Veterinary Microbiology; 222; 8-2018; 30-38; CONICET Digital; CONICET

الإتاحة: https://doi.org/10.1016/j.vetmic.2018.06.016Test
http://hdl.handle.net/11336/117550Test