Identification of Mx gene nucleotide dimorphism (G/A) as genetic marker for antiviral activity in Egyptian chickens
| العنوان: | Identification of Mx gene nucleotide dimorphism (G/A) as genetic marker for antiviral activity in Egyptian chickens |
|---|---|
| المؤلفون: | Mohamed S. Hassanane, Fatma Ahmed, Nagwa H. A. Hassan, Esteftah M. El-Komy, Amal A. M. Hassan, Khaled M. Roushdy |
| المصدر: | Journal of Genetic Engineering and Biotechnology, Vol 16, Iss 1, Pp 83-88 (2018) Journal of Genetic Engineering & Biotechnology |
| بيانات النشر: | SpringerOpen, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Genotyping, lcsh:QH426-470, lcsh:Biotechnology, Biology, PCR-RFLP, 03 medical and health sciences, lcsh:TP248.13-248.65, Genotype, General Materials Science, Antiviral activity, Allele, Gene, Allele frequency, Genetics, 0402 animal and dairy science, 04 agricultural and veterinary sciences, Mx gene, Amplicon, 040201 dairy & animal science, Molecular biology, Animal Biotechnology, Egyptian chickens, Restriction enzyme, lcsh:Genetics, 030104 developmental biology, Genetic marker |
| الوصف: | Egyptian chickens, representing 2 breeds and 7 strains, were genotyped using the PCR-RFLP and sequencing techniques for detection of a non-synonymous dimorphism (G/A) in exon 14 of chicken Myxovirus resistance (Mx) gene. This dimorphic position is responsible for altering Mx protein’s antiviral activity. Polymerase Chain reactions were performed using Egyptian chickens DNA and specific primer set to amplify Mx DNA fragments of 299 or 301 bp, containing the dimorphic position. Amplicons were cut with restriction enzyme Hpy81. Genotype and allele frequencies for the resistant allele A and sensitive allele G were calculated in all the tested chickens. Results of PCR-RFLP were confirmed by sequencing. The three genotypes AA, AG, GG at the target nucleotide position in Mx gene were represented in all the studied Egyptian chicken breeds and strains except Baladi strain which showed only one genotype AA. The average allele frequency of the resistant A allele in the tested birds (0.67) was higher than the sensitive G allele average frequency in the same birds (0.33). Appling PCR-RFLP technique in the breeding program can be used to select chickens carrying the A allele with high frequencies. This will help in improving poultry breeding in Egypt by producing infectious disease-resistant chickens. Keywords: Egyptian chickens, Antiviral activity, Mx gene, Genotyping, PCR-RFLP |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::726e2259ac7b34061e4517fd0da6ff51Test http://www.sciencedirect.com/science/article/pii/S1687157X17301087Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....726e2259ac7b34061e4517fd0da6ff51 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |