(Sorensen buffer, pH7.4, 37°C) with (a) cocaine, morphine and methadone, (b) with the oxidative enzymes, and (c) with cocaine, morphine and methadone in the presence of oxidative enzymes. The oxidative enzymes were PHS purified from ram seminal vesic1es (20 U/mg), and SIO Mix liver microsomes from phenobarbital treated rats (4 mg/mi protein). The reaction was stopped by chilling the tubes at 0-4 oe. After centrifugation, histamine and lactate dehydrogenase (LDH) were analysed in the supernatants and in the pellets (fluorimetric and spectrophotometric assays, respectively) and expressed as percent of the total histamine content. MDA was measured by a spectrophotometric assay [4].
