دورية أكاديمية
Critical analytical evaluation of promising markers for sarcopenia
| العنوان: | Critical analytical evaluation of promising markers for sarcopenia |
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| المؤلفون: | Cavalier, Etienne, Beaudart, Charlotte, Buckinx, Fanny, Bruyère, Olivier, Reginster, Jean-Yves |
| المصدر: | European Geriatric Medicine, 7 (3), 239-242 (2016) |
| بيانات النشر: | Elsevier Masson SAS |
| سنة النشر: | 2016 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | Biomarkers, Frailty, Sarcopenia, Article, Human health sciences, General & internal medicine, Sciences de la santé humaine, Médecine générale & interne |
| الوصف: | peer reviewed ; We tested and validated irisin (IRI), myostatin (MYO), PIIINP, osteoglycin (OGN), TMEM119 (TMEM) and activin A (AA) and established the analytical performance, reference range and stability (considered unstable if more than 20% increase/decrease in the levels was observed in more than 10% of the samples). We were unable to obtain a valuable calibration curve with the Cusabio kits (TMEME and OGN). Coefficient of variation (CV) was too high for IRI (CV 17-30%), but were ≤ 10% for the 3 other analytes. AA and MYO were stable up to 3 months at -20 °C and -80 °C in serum or EDTA plasma and up to 6 months at -80 8C. PIIINP was stable only 1 month in EDTA plasma (but not in serum) at -20 °C or -80 8C. After 3 months of storage, PIIINP was not stable anymore, in serum or EDTA plasma, at -20 °C or -80 8C. Surprisingly, after 6 months at -80 8C, results returned in the ± 20% for both serum and EDTA plasma. PIIINP levels did not differ between men and women and the RR was (median, 90% CI) 1.2 (0.8-1.6)-6.0 (5.6-6.4) μg/L. The RR for MYO was 845 (437-1312)-6067 (5524-6552) pg/mL for men and 600 (268-1027)-4438 (4026-4837) pg/mL for women and the RR for AA was 177 (132-210)-622 (580-661) pg/mL for men and 98 (49-147)-480 (430-525) pg/mL for women. PIIINP and AA but not MYO accumulated in CKD as values observed in 10 hemodialyzed patients were higher than in normal individuals. © 2015 Elsevier Masson SAS and European Union Geriatric Medicine Society. All rights reserved. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 1878-7649 1878-7657 |
| العلاقة: | urn:issn:1878-7649; urn:issn:1878-7657; https://orbi.uliege.be/handle/2268/216655Test; info:hdl:2268/216655; scopus-id:2-s2.0-84949667540 |
| DOI: | 10.1016/j.eurger.2015.11.002 |
| الإتاحة: | https://doi.org/10.1016/j.eurger.2015.11.002Test https://orbi.uliege.be/handle/2268/216655Test |
| حقوق: | restricted access ; http://purl.org/coar/access_right/c_16ecTest ; info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.FCDE7D98 |
| قاعدة البيانات: | BASE |
| تدمد: | 18787649 18787657 |
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| DOI: | 10.1016/j.eurger.2015.11.002 |