دورية أكاديمية
How to make usage of the standardized EuroFlow 8-color protocols possible for instruments of different manufacturers
| العنوان: | How to make usage of the standardized EuroFlow 8-color protocols possible for instruments of different manufacturers |
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| المؤلفون: | Novakova, Michaela, Glier, Hana, Brdičková, Naděžda, Vlková, Marcela, Santos, Ana Helena, Lima, Margarida, Roussel, Mikaël, Flores-Montero, Juan, Szczepanski, Tomasz, Böttcher, Sebastian, van Der Velden, Vincent H J, Fernandez, Paula, Mejstříková, Ester, Burgos, Leire, Paiva, Bruno, van Dongen, Jacques J M, Orfao, Alberto, Kalina, Tomáš |
| المساهمون: | Childhood Leukaemia Investigation Prague (CLIP), University Hospital Motol Prague, Microenvironment, Cell Differentiation, Immunology and Cancer (MICMAC), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), University of Rostock, Erasmus University Medical Center Rotterdam (Erasmus MC), Clínica Universidad de Navarra Pamplona, Leiden University Medical Center (LUMC), Universiteit Leiden = Leiden University, 15-28525A, Ministerstvo Zdravotnictví Ceské Republiky, Ministerio de Economía y Competitividad, Ministry of Education, Youth and Sports, Czech Republic, CZ.2.16/3.1.00/24505, European Structural funds, 802214, GAUK, CB16/12/00400, Instituto de Salud Carlos II |
| المصدر: | ISSN: 0022-1759 ; Journal of Immunological Methods ; https://univ-rennes.hal.science/hal-01875353Test ; Journal of Immunological Methods, 2019, 475, pp.112388. ⟨10.1016/j.jim.2017.11.007⟩. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2019 |
| المجموعة: | Université de Rennes 1: Publications scientifiques (HAL) |
| مصطلحات موضوعية: | Flow cytometry, Fluorescent spectrum, Fluorochromes, Standardization, EuroFlow, [SDV.CAN]Life Sciences [q-bio]/Cancer |
| الوصف: | International audience ; A critical component of the EuroFlow standardization of leukemia/lymphoma immunophenotyping is instrument setup. Initially, the EuroFlow consortium developed a step-by-step standard operating protocol for instrument setup of ≥8-color flow cytometers that were available in 2006, when the EuroFlow activities started. Currently, there are 14 instruments from 9 manufacturers capable of 3-laser excitation and ≥8 color measurements. The specific adaptations required in the instrument set-up to enable them to acquire the standardized 8-color EuroFlow protocols are described here. Overall, all 14 instruments can be fitted with similar violet, blue and red lasers for simultaneous measurements of ≥8 fluorescent dyes. Since individual instruments differ both on their dynamic range (scale) and emission filters, it is not accurate to simply recalculate the target values to different scale, but adjustment of PMT voltages to a given emission filter and fluorochrome, is essential. For this purpose, EuroFlow has developed an approach using Type IIB (spectrally matching) particles to set-up standardized and fully comparable fluorescence measurements, in instruments from different manufacturers, as demonstrated here for the FACSCanto II, and Navios and MACSQuant flow cytometers. Data acquired after such adjustment on any of the tested cytometry platforms could be fully superimposed and therefore analyzed together. The proposed approach can be used to derive target values for any combination of spectrally distinct fluorochromes and any distinct emission filter of any new flow cytometry platform, which enables the measurement of the 8-color EuroFlow panels in a standardized way, by creating superimposable datafiles. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/29154914; hal-01875353; https://univ-rennes.hal.science/hal-01875353Test; https://univ-rennes.hal.science/hal-01875353/documentTest; https://univ-rennes.hal.science/hal-01875353/file/Novakova_How%20to%20make%20usage%20of%20the%20standardized%20EuroFlow.pdfTest; PUBMED: 29154914 |
| DOI: | 10.1016/j.jim.2017.11.007 |
| الإتاحة: | https://doi.org/10.1016/j.jim.2017.11.007Test https://univ-rennes.hal.science/hal-01875353Test https://univ-rennes.hal.science/hal-01875353/documentTest https://univ-rennes.hal.science/hal-01875353/file/Novakova_How%20to%20make%20usage%20of%20the%20standardized%20EuroFlow.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.64642363 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jim.2017.11.007 |
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