المؤلفون: Yu, Shaoyang, Yang, Yating, Hu, Gaoya, Wang, Wei, Zhuang, Wei, Wu, Yong, Huang, Rongfu, Zhang, Yongquan, Gong, Sisi, Fan, Chunmei

المصدر: Analytical Letters; 2023, Vol. 56 Issue 18, p2910-2920, 11p

مصطلحات موضوعية: ALKALINE phosphatase, ACID phosphatase, FOURIER transform infrared spectroscopy, DUAL fluorescence, TRANSMISSION electron microscopy

مستخلص: A novel fluorescence and colorimetric dual probe based upon the inner filter effect (IFE) is reported to selectively and sensitively determine alkaline phosphatase (ALP). First, up-conversion nanoparticles (UCNPs) were prepared via a facile hydrothermal method and characterized by transmission electron microscopy (TEM), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FT-IR), and zeta potential. Under near infrared (NIR) excitation at 980 nm, the prepared nanoparticles emit green light at 541 nm. The absorption band of p-phenylenediamine (PPD) overlaps the emission of UCNPs, which effectively quenches the fluorescence of UCNPs. However, ascorbic acid (AA) generated from the reaction of ascorbic acid 2-phosphate (AAP) and ALP impedes the oxidation of PPD and the fluorescence is restored. Based on this change, a "turn-on" sensor was used to determine AA and ALP with the limits of detection of 2.49 μM and 0.018 U/L, respectively. The constructed assay was applied to determine ALP in serum and the recoveries were from 93.3% to 101.3% with a relative standard deviation from 1.1% to 3.3%. A facile and efficient approach is reported for the determination ALP and offers a theoretical basis for the related diseases. [ABSTRACT FROM AUTHOR]

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المؤلفون: Chen, Haiyan, Li, Qiaoyin, You, Shumin, Huang, Xuemin, Fan, Chunmei, Lin, Zhenyu, Qiu, Bin

المصدر: Microchimica Acta; 9/1/2022, Vol. 189 Issue 9, p1-8, 8p

مصطلحات موضوعية: TETRACYCLINES, TETRACYCLINE, FLUORESCENCE resonance energy transfer, FLUORESCENCE, ANTIBIOTICS

مستخلص: A ratiometric fluorescence probe is proposed for sensitive and visual detection of tetracyclinee (TC) based on cascade fluorescence signal amplification induced by bovine serum albumin–stabilized copper nanoclusters (BSA-CuNCs) and yttrium ions (Y³⁺). TC can combine with Y³⁺ to form the complex (TC-Y³⁺) to enhance the fluorescence of TC at 515 nm. Then, positively charged TC-Y³⁺ and negatively charged BSA-CuNCs was bonded together by electrostatic interactions to achieve the fluorescence resonance energy transfer (FRET) process. With the increase of TC concentration, the fluorescence intensity of TC-Y³⁺ at 515 nm (F515) gradually increased; meanwhile, the fluorescence intensity of BSA-CuNCs at 405 nm (F405) decreased gradually. The ratio of F515 and F405 was used for the quantitative determination of TC. The linear range of the constructed fluorescent probe is 1.0 to 60.0 μM, and the limit of detection is 0.22 μM. The method was successfully applied to the determination of TC in spiked milk with recoveries ranging from 94.3 to 112%. Furthermore, the color of this platform can be observed from dark violet to bright green under the UV lamp. Since the response time of the reaction is less than 10 s, an intelligent sensing platform based on the use of the smartphone as image acquisition equipment was also established to realize rapid on-site and portable detection of TC through the colorimetric recognition application. [ABSTRACT FROM AUTHOR]

: Copyright of Microchimica Acta is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)