5′ Untranslated region of the Hsp12 gene contributes to efficient translation in Aspergillus oryzae
| العنوان: | 5′ Untranslated region of the Hsp12 gene contributes to efficient translation in Aspergillus oryzae |
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| المؤلفون: | Toshitaka Minetoki, Masato Hirotsune, Takayuki Bogaki, Akio Koda |
| المصدر: | Applied Microbiology and Biotechnology. 70:333-336 |
| بيانات النشر: | Springer Science and Business Media LLC, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Untranslated region, Regulation of gene expression, Base Sequence, biology, Five prime untranslated region, Aspergillus oryzae, Recombinant Fusion Proteins, Molecular Sequence Data, fungi, food and beverages, General Medicine, biology.organism_classification, Applied Microbiology and Biotechnology, Molecular biology, Genetic translation, Gene Expression Regulation, Fungal, Protein Biosynthesis, Gene expression, Protein biosynthesis, 5' Untranslated Regions, Gene, Heat-Shock Proteins, Glucuronidase, Biotechnology |
| الوصف: | We describe a 5' untranslated region (5'UTR) that dramatically increases the expression level of an exogenous gene in Aspergillus oryzae. Using a series of 5'UTR::GUS (uidA) fusion constructs, we analyzed the translation efficiency of chimeric mRNAs with different 5'UTRs at different temperatures. We found that the 5'UTR of a heat-shock protein gene, Hsp12, greatly enhanced the translation efficiency of the chimeric GUS mRNA at normal temperature (30 degrees C). Moreover, at high temperature (37 degrees C), the translation efficiency of the mRNA containing the Hsp12 5'UTR was far superior to that of mRNAs containing nonheat-shock 5'UTRs, resulting in much more efficient expression of GUS protein (about 20-fold higher GUS activity compared to the control construct). This 5'UTR can be used in combination with various strong promoters to enhance the expression of foreign proteins in A. oryzae. |
| تدمد: | 1432-0614 0175-7598 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::895cfbcdce545269c0f878e9745f8bf4Test https://doi.org/10.1007/s00253-005-0083-xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....895cfbcdce545269c0f878e9745f8bf4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320614 01757598 |
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