التفاصيل البيبلوغرافية
| العنوان: |
The developmental regulator Pcz1 affects the production of secondary metabolites in the filamentous fungus Penicillium roqueforti. |
| المؤلفون: |
Rojas-Aedo, Juan F.1, Gil-Durán, Carlos1, Goity, Alejandra2, Vaca, Inmaculada3, Levicán, Gloria1, Larrondo, Luis F.2, Chávez, Renato1 renato.chavez@usach.cl |
| المصدر: |
Microbiological Research. Jul2018, Vol. 212, p67-74. 8p. |
| مصطلحات موضوعية: |
*PENICILLIUM roqueforti, *FUNGAL metabolites, *FUNGAL development, *CHEESE varieties, *FILAMENTOUS fungi |
| مستخلص: |
Penicillium roqueforti is used in the production of several kinds of ripened blue-veined cheeses. In addition, this fungus produces interesting secondary metabolites such as roquefortine C, andrastin A and mycophenolic acid. To date, there is scarce information concerning the regulation of the production of these secondary metabolites. Recently, the gene named pcz1 ( Penicillium C6 zinc domain protein 1) was described in P. roqueforti , which encodes for a Zn(II) 2 Cys 6 protein that controls growth and developmental processes in this fungus. However, its effect on secondary metabolism is currently unknown. In this work, we have analyzed how the overexpression and down-regulation of pcz1 affect the production of roquefortine C, andrastin A and mycophenolic acid in P. roqueforti . The three metabolites were drastically reduced in the pcz1 down-regulated strains. However, when pcz1 was overexpressed, only mycophenolic acid was overproduced while, on the contrary, levels of roquefortine C and andrastin A were diminished. Importantly, these results match the expression pattern of key genes involved in the biosynthesis of these metabolites. Taken together, our results suggest that Pcz1 plays a key role in regulating secondary metabolism in the fungus Penicillium roqueforti . [ABSTRACT FROM AUTHOR] |
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