A physiological and molecular study of the effects of nickel deficiency and phenylphosphorodiamidate (PPD) application on urea metabolism in oilseed rape (Brassica napus L.)
| العنوان: | A physiological and molecular study of the effects of nickel deficiency and phenylphosphorodiamidate (PPD) application on urea metabolism in oilseed rape (Brassica napus L.) |
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| المؤلفون: | Maria Garnica, Jean-Claude Yvin, Philippe Laîné, Mustapha Arkoun, Céline Masclaux-Daubresse, Laëtitia Jannin, Philippe Etienne, José-Maria Garcia-Mina, Sylvie Citerne, Alain Ourry |
| المساهمون: | Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Timac Agro Spain, Timac - Agro nternational, Pole de competitivite Mer-Bretagne, Fond Unique Inter-ministeriel, Ecophysiologie Végétale, Agronomie et Nutritions ( EVA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Institut Jean-Pierre Bourgin ( IJPB ), Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech, Timac Agro Int |
| المصدر: | Plant and Soil Plant and Soil, Springer Verlag, 2013, 362 (1-2), pp.79-92. ⟨10.1007/s11104-012-1227-2⟩ Plant and Soil, Springer Verlag, 2013, 362 (1-2), pp.79-92. 〈10.1007/s11104-012-1227-2〉 |
| بيانات النشر: | HAL CCSD, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | 0106 biological sciences, NITROGEN-METABOLISM, BnDUR3, Urease, Enzymatic activities, [SDV]Life Sciences [q-bio], Soil Science, Plant Science, engineering.material, 01 natural sciences, HIGHER-PLANTS, 03 medical and health sciences, chemistry.chemical_compound, Ammonia, N-15-labeling, Nickel, PHYTOTOXICITY, Urea, Ammonium, VOLATILIZATION, Food science, MICROBIAL UREASES, IN-VIVO, 030304 developmental biology, 2. Zero hunger, Phenylphosphorodiamidate (PPD), 0303 health sciences, TOBACCO, AMMONIA, biology, [ SDV ] Life Sciences [q-bio], GLUTAMATE-DEHYDROGENASE, Glutamate dehydrogenase, ARABIDOPSIS, Urea transport, chemistry, Agronomy, engineering, biology.protein, Phytotoxicity, Fertilizer, Gene expressions, 010606 plant biology & botany |
| الوصف: | Urea is the major nitrogen (N) form supplied as fertilizer in agriculture. However, urease, a nickel-dependent enzyme, allows plants to use external or internally generated urea as a nitrogen source. Since a urease inhibitor is frequently applied in conjunction with urea fertilizer, the N-metabolism of plants may be affected. The aim of this study was to determine physiological and molecular effects of nickel deficiency and a urease inhibitor on urea uptake and assimilation in oilseed rape. Plants were grown on hydroponic solution with urea as the sole N source under three treatments: plants treated with nickel (+Ni) as a control, without nickel (-Ni) and with nickel and phenylphosphorodiamidate (+Ni+PPD). Urea transport and assimilation were investigated. The results show that Ni-deficiency or PPD supply led to reduced growth and reduced N-15-uptake from urea. This effect was more pronounced in PPD-treated plants, which accumulated high amounts of urea and ammonium. Thus, Ni-deficiency or addition of PPD, limit the availability of N and decreased shoot and root amino acid content. The up-regulation of BnDUR3 in roots indicated that this gene is a component of the stress response to nitrogen-deficiency. A general decline of glutamine synthetase (GS) activity and activation of glutamate dehydrogenase (GDH) and increases in its expression level were observed in control plants. At the same time, in (-N) or (+Ni+PPD) treated plants, no increases in GS or GDH activities and expression level were found. Overall results showed that plants require Ni as a nutrient (while most widely used nutrient solutions are devoid of Ni), whether they are grown with or without a urea supply, and that urease inhibitors may have deleterious effects at least in hydroponic grown oilseed rape. |
| اللغة: | English |
| تدمد: | 0032-079X 1573-5036 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0cc1284adb511627a408e631081c6c9Test https://hal.archives-ouvertes.fr/hal-01190672Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....d0cc1284adb511627a408e631081c6c9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 0032079X 15735036 |
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