المؤلفون: Kang Li, Huanhuan Chen, Jianjun Li, Yi Feng, Guanghua Lan, Shujia Liang, Meiliang Liu, Abdur Rashid, Hui Xing, Zhiyong Shen, Yiming Shao

المصدر: Emerging Microbes & Infections
article-version (VoR) Version of Record
Emerging Microbes and Infections, Vol 11, Iss 1, Pp 158-167 (2022)

مصطلحات موضوعية: Adult, CD4-Positive T-Lymphocytes, Male, Receptors, CXCR4, Genotype, Epidemiology, Anti-HIV Agents, Immunology, antiretroviral therapy, HIV Infections, Infectious and parasitic diseases, RC109-216, HIV Envelope Protein gp120, Microbiology, coreceptor tropism, Immune Reconstitution, Virology, Antiretroviral Therapy, Highly Active, Drug Discovery, Humans, Retrospective Studies, genetic sub-cluster, virus diseases, General Medicine, Middle Aged, QR1-502, Peptide Fragments, CD4 Lymphocyte Count, immune reconstruction, Viral Tropism, Infectious Diseases, HIV-1, Parasitology, Female, Research Article

الوصف: There are great disparities of the results in immune reconstruction (IR) of the HIV-1 infected patients during combined antiretroviral therapy (cART), due to both host polymorphisms and viral genetic subtypes. Identifying these factors and elucidating their impact on the IR could help to improve the efficacy. To study the factors influencing the IR, we conducted a 15-year retrospective cohort study of HIV-1 infected individuals under cART. The trend of CD4+ count changes was evaluated by the generalized estimating equations. Cox proportional model and propensity score matching were used to identify variables that affect the possibility of achieving IR. The tropism characteristics of virus were compared using the coreceptor binding model. In addition to baseline CD4+ counts and age implications, CRF01_AE cluster 1 was associated with a poorer probability of achieving IR than infection with cluster 2 (aHR, 1.39; 95%CI, 1.02-1.90) and other subtypes (aHR, 1.83; 95%CI, 1.31-2.56). The mean time from cART initiation to achieve IR was much longer in patients infected by CRF01_AE cluster 1 than other subtypes/sub-clusters (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e621126de395550ab19b4a054ba37ff8Test
https://pubmed.ncbi.nlm.nih.gov/34895083Test

المؤلفون: Qin He, Shujia Liang, Qiuying Zhu, Kailing Tang, Xiuling Wu, Zhiyong Shen, Jinghua Huang, Xianwu Pang, Xinjuan Zhou, Ningye Fang

المصدر: BMC Infectious Diseases, Vol 21, Iss 1, Pp 1-9 (2021)
BMC Infectious Diseases

مصطلحات موضوعية: Adult, Male, 0301 basic medicine, China, medicine.medical_specialty, Genotype, Human immunodeficiency virus 1, HIV Infections, Drug resistance, 010502 geochemistry & geophysics, Logistic regression, 01 natural sciences, lcsh:Infectious and parasitic diseases, law.invention, 03 medical and health sciences, Antiretroviral resistance, Medical microbiology, Risk Factors, law, Drug Resistance, Viral, HIV drug resistance, Prevalence, medicine, Humans, lcsh:RC109-216, Phylogeny, 0105 earth and related environmental sciences, Primary antiretroviral resistance, business.industry, Odds ratio, Transmitted drug resistance, 030112 virology, Antiretroviral therapy, Logistic Models, Infectious Diseases, Transmission (mechanics), Anti-Retroviral Agents, pol Gene Products, Human Immunodeficiency Virus, Tropical medicine, HIV-1, Female, business, Research Article, Demography

الوصف: Background The widespread use of antiretroviral therapy (ART) has resulted in the development of transmitted drug resistance (TDR), which reduces ART efficacy. We explored TDR prevalence and its associated risk factors in newly diagnosed individuals in Guangxi. Methods We enrolled 1324 participants who were newly diagnosed with HIV-1 and had not received ART at voluntary counselling and testing centres (VCT) in Guangxi, China, who had not received ART. Phylogenetic relationship, transmission cluster, and genotypic drug resistance analyses were performed using HIV-1 pol sequences. We analysed the association of demographic and virological factors with TDR. Results In total, 1151 sequences were sequenced successfully, of which 83 (7.21%) showed evidence of TDR. Multivariate logistic regression analysis revealed that there was significant difference between the prevalence of TDR and unmarried status (adjusted odds ratio (aOR) = 2.41, 95% CI: 1.23–4.71), and CRF08_BC subtype (aOR = 2.03, 95% CI: 1.13–3.64). Most cases of TDR were related to resistance to non-nucleoside reverse transcriptase inhibitors (4.87%) and V179E was the most common mutation detected. We identified a total of 119 HIV transmission clusters (n = 585, 50.8%), of which 18 (15.1%) clusters showed evidence of TDR (36, 41.86%). Three clusters were identified that included drug-resistant individuals having a transmission relationship with each other. The following parameters were associated with TDR transmission risk: Unmarried status, educational level of junior high school or below, and CRF08_BC subtype may be a risk of the transmission of TDR. Conclusions Our findings indicated that moderate TDR prevalence and highlighted the importance of continuous TDR monitoring and designing of strategies for TDR mitigation.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c30455ee3ec191cc5a79c28aaddaed31Test
https://doi.org/10.1186/s12879-021-05854-wTest

المؤلفون: Yanpeng Li, Rongge Yang, Ting Yuan, Ulf Dittmer, Shujia Liang, Daniel Hoffmann, Hongye Wang, Feng Qian, Tingting Li, Binlian Sun, Chuanwu Zhu

المصدر: Archives of Virology. 163:3303-3315

مصطلحات موضوعية: Adult, Male, 0301 basic medicine, Glycan, Human immunodeficiency virus (HIV), HIV Infections, Antibodies, Viral, medicine.disease_cause, Neutralization, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Neutralization Tests, Virology, medicine, Humans, 030212 general & internal medicine, Viral immunology, Phylogeny, Aged, biology, Plasma samples, env Gene Products, Human Immunodeficiency Virus, virus diseases, General Medicine, Middle Aged, Antibodies, Neutralizing, 030104 developmental biology, Mathematik, HIV-1, biology.protein, Female, Antibody, Biologie

الوصف: Broadly neutralizing antibodies (bNAbs) are very promising agents for HIV-1 prophylaxis and AIDS treatment. However, the neutralization susceptibility of circulating recombinants such as CRF01_AE, which is becoming increasingly prevalent, has not been studied in detail until now. Here, we focused on CRF01_AE in China and aimed to find bNAbs that can be used for neutralization of CRF01_AE. Full-length env clones were obtained from the plasma samples of 22 HIV-1-infected individuals sampled in 2009 and 2015. An env-pseudovirus-based neutralization assay was conducted using five categories of bNAbs: VRC01, NIH45-46G54W, and 3BNC117 (targeting the CD4 binding site); PG9 and PG16 (targeting the V1V2 loop); 2G12 (glycan specific), PGT121 and 10-1074 (targeting the V3 glycan); 2F5, 4E10, and 10E8 (targeting the membrane-proximal external region (MPER)). The neutralizing efficiency was compared, and features of the escape pseudoviruses were analyzed. The CRF01_AE pseudoviruses exhibited different susceptibility to these bNAbs. Overall, 4E10, 10E8, and 3BNC117 neutralized all 22 env-pseudotyped viruses, followed by NIH45-46G54W and VRC01, which neutralized more than 90% of the viruses. 2F5, PG9, and PG16 showed only moderate breadth, while the other three bNAbs neutralized none of these pseudoviruses. Specifically, 10E8, NIH45-46G54Wand 3BNC117 showed the highest efficiency, combining neutralization potency and breadth. Mutations at position 160, 169, 171 were associated with resistance to PG9 and PG16, while loss of a potential glycan at position 332 conferred insensitivity to V3-glycan-targeting bNAbs. Our results may help for choosing bNAbs that can be used preferentially for prophylactic or therapeutic approaches in China.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6a238be0c4918e005dcb81c99f4ac00Test
https://doi.org/10.1007/s00705-018-4011-7Test

المؤلفون: Qiuying Zhu, Guanghua Lan, Huanhuan Chen, Yi Feng, Yiming Shao, Xinjuan Zhou, Zhiyong Shen, Shujia Liang, Yuhua Ruan, Jianjun Li, Lingjie Liao, Zhiqiang Cao, Chang Song, Hui Xing

المصدر: Scientific Reports
Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)

مصطلحات موضوعية: 0301 basic medicine, Adult, Male, medicine.medical_specialty, Adolescent, Genotype, Human immunodeficiency virus (HIV), lcsh:Medicine, HIV Infections, medicine.disease_cause, Article, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Medical research, Internal medicine, Medicine, Humans, In patient, 030212 general & internal medicine, Cd4 cell count, lcsh:Science, Multidisciplinary, business.industry, Proportional hazards model, lcsh:R, Significant difference, Hazard ratio, virus diseases, Middle Aged, Antiretroviral therapy, CD4 Lymphocyte Count, Computational biology and bioinformatics, 030104 developmental biology, Anti-Retroviral Agents, Risk factors, HIV-1, lcsh:Q, Female, business

الوصف: To assess whether human immunodeficiency virus type 1 (HIV-1) genotype influences baseline CD4+ T lymphocyte (CD4+) cell count and mortality of patients. The study was conducted from 2014 to 2019 in Guangxi, China, and included 2845 newly diagnosed HIV patients. We used a median regression model to compare CD4+ cell counts in patients newly diagnosed with different HIV-1 genotypes, and a Cox regression model to analyze the associations between HIV-1 genotypes and mortality before and after antiretroviral treatment (ART). In newly diagnosed HIV patients, the baseline CD4+ cell counts of patients with CRF01_AE were significantly lower than those of patients with CRF07_BC, CRF08_BC, and other genotypes. Compared with CRF01_AE, patients infected with CRF07_BC (hazard ratio, 0.55; 95% CI 0.36–0.85), CRF08_BC (hazard ratio, 0.67; 95% CI 0.52–0.85), or other genotypes (hazard ratio, 0.52; 95% CI 0.29–0.94) had significantly lower mortality rates before ART. There were no significant associations between different HIV-1 genotypes and mortality after ART. HIV-1 genotype significantly influences baseline CD4+ cell count and mortality before ART in newly diagnosed HIV patients. We find no significant difference in the outcome of death after ART in patients with different HIV-1 genotypes.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cef29d367f431e7591e06d5e700c90f5Test
https://pubmed.ncbi.nlm.nih.gov/32985559Test

المؤلفون: Lin Xiao, Xiao-Guang Sun, Lin Chen, Ling-Jie Liao, Xiaoqin Xu, Hui Xing, Shui-Bin Luo, Hongyan Lu, Ruihua Kang, Guohui Wu, Yanling Ma, Shujia Liang, Shu Liang, Jianmei He, Yiming Shao, Yuhua Ruan, Xin-Hui Zhang

المصدر: Infectious Diseases of Poverty
Infectious Diseases of Poverty, Vol 9, Iss 1, Pp 1-9 (2020)

مصطلحات موضوعية: 0301 basic medicine, Adult, Male, medicine.medical_specialty, China, Anti-HIV Agents, 030106 microbiology, Human immunodeficiency virus (HIV), Pretreatment drug resistance, HIV Infections, Drug resistance, Logistic regression, medicine.disease_cause, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Internal medicine, Drug Resistance, Viral, Prevalence, Medicine, Humans, lcsh:RC109-216, 030212 general & internal medicine, Transmission network, Aged, Aged, 80 and over, business.industry, Transmission (medicine), lcsh:Public aspects of medicine, Public Health, Environmental and Occupational Health, HIV, lcsh:RA1-1270, General Medicine, Odds ratio, Middle Aged, Antiretroviral therapy, Infectious Diseases, Cross-Sectional Studies, Tropical medicine, Female, business, HIV drug resistance, Research Article

الوصف: BackgroundAfter the scale-up of antiretroviral therapy (ART) for HIV infected people, increasing numbers of patients have pretreatment drug resistance (PDR). In this study, the prevalence of PDR was evaluated in adults initiating antiretroviral therapy in China.MethodsBlood samples were obtained from 1943 patients who initiated antiretroviral therapy (ART) in 2017 from 13 provinces or cities in China. Pol sequences were used to analyze drug resistance and construct transmission networks. Logistic regression model was used to estimate the potential factors associated with PDR.ResultsIn total, 1711 eligible patients (76.0% male; 87.8% aged ≥ 25 years) were included, of which 117 (6.8%) had PDR. The highest rates of PDR were 12.2% in Liangshan Prefecture of Sichuan and 9.3 and 8.9% in Dehong and Lincang Prefecture of Yunnan. A multivariate logistic regression analysis revealed that PDR was significantly higher among intravenous drug users (adjusted Odds Ratio (aOR) = 2.64, 95%CI: 1.57–4.44) and individuals from Liangshan, Dehong, and Lincang (aOR = 2.04, 95%CI: 1.26–3.30). In total, 754 sequences were used to generate 164 transmission networks. Five transmission networks had two or three sequences containing the same mutations, two networks contained subjects from Liangshan, and one network contained subjects from Dehong.ConclusionsOverall, the PDR prevalence was moderate, with a particularly high prevalence in areas with severe HIV epidemics. These results indicate the importance of continuous PDR monitoring in patients initiating antiretroviral therapy.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8d76933cba682e00810e865e18b42afTest
http://europepmc.org/articles/PMC7247188Test

المؤلفون: Desheng Kong, Yan Wang, Chen Wang, Liying Ma, Yi Feng, Shujia Liang

المصدر: AIDS Research and Human Retroviruses. 34:635-639

مصطلحات موضوعية: Male, 0301 basic medicine, China, medicine.medical_specialty, Genotype, viruses, Human Immunodeficiency Virus Proteins, Immunology, Human immunodeficiency virus (HIV), HIV Infections, Biology, medicine.disease_cause, Recombinant virus, law.invention, 03 medical and health sciences, 0302 clinical medicine, law, Virology, Molecular genetics, medicine, Humans, 030212 general & internal medicine, Gene, Recombination, Genetic, Genetics, Genetic diversity, Whole Genome Sequencing, virus diseases, Middle Aged, Viral Tropism, 030104 developmental biology, Infectious Diseases, HIV-1, Recombinant DNA, Female, Identification (biology), human activities

الوصف: Continual generation of HIV-1 recombinant forms might contribute to its genetic diversity. In this study, a novel B/C/CRF01_AE recombinant virus (GX2016EU13) was identified, which was isolated from a HIV-positive man who was infected through heterosexual sex in Guangxi, China. GX2016EU13 was identified as CCR5-tropic. As shown in the near full-length genome (NFLG) analyses, five recombinant breakpoints were found only in the pol gene of GX2016EU13, which divided the NFLG into three subtype B segments, two subtype C segments, and one CRF01_AE segment. The whole gag sequence of GX2016EU13 belongs to subtype B, and the whole env sequence of GX2016EU13 belongs to CRF01_AE. The recombinant form was distinct from the other circulating recombinant forms and unique recombinant forms reported. The emergence of GX2016EU13 may suppose the complexity of the HIV-1 epidemic in high-risk sexual populations in Guangxi, China.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46c411d24b66f4331813173ecf212a63Test
https://doi.org/10.1089/aid.2018.0070Test

المؤلفون: Huanhuan Chen, Yuhua Ruan, Yinghui You, Yiming Shao, Guanghua Lan, Xiaoyi Yang, Zhenzhu Tang, Zhiyong Shen, Yi Chen, Lingjie Liao, Hui Xing, Pengtao Liu, Qiuying Zhu, Yi Feng, Shujia Liang

المصدر: Scientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
Scientific Reports

مصطلحات موضوعية: Adult, Male, 0301 basic medicine, China, medicine.medical_specialty, lcsh:Medicine, HIV Infections, Lower risk, Article, Medication Adherence, Cohort Studies, 03 medical and health sciences, 0302 clinical medicine, Risk Factors, Internal medicine, Secondary Prevention, medicine, Humans, 030212 general & internal medicine, Hiv transmission, lcsh:Science, Retrospective Studies, Multidisciplinary, business.industry, Transmission (medicine), Public health, lcsh:R, Middle Aged, Antiretroviral therapy, CD4 Lymphocyte Count, Sexual Partners, 030104 developmental biology, Anti-Retroviral Agents, Serodiscordant, HIV-1, Female, lcsh:Q, business, Viral load, Cohort study

الوصف: Early antiretroviral therapy (ART) initiation is a recommended public health approach for the prevention of HIV-1 transmission. In this cohort study, we included 13132 serodiscordant couples. ART was initiated for patients with CD4+ T cell counts less than 200 cells/uL, 350 cells/uL, and 500 cells/uL respectively. This divided the ART treated couples into three groups. Univariate and multivariate intention-to-treat analyses were performed to examine the association between the study groups. Early-ART initiation was associated with a 45% lower risk of partner infection than was late-ART initiation (AHR 0.55, 95% CI, 0.37–0.81). Mid-ART initiation was associated with a 39% lower risk of partner infection than was late-ART initiation (AHR 0.61, 95% CI, 0.48–0.78). However, the risk reduction between the early and mid-ART groups was not significant. Drug compliance (AHR 1.55, 95% CI 1.03–2.35) and increased baseline viral load (AHR 1.41, 95% CI 1.33–1.51) were associated with an increased risk of infections among partners in the treatment. Prevention of HIV transmission as a result of early ART initiation was feasible on national and regional scales; however, many factors, such as the motivation to commence ART, adherence, and attrition, may affect the impact of this strategy in programmatic settings.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2a9d9ec00d635bbeec1dbaf2237babfTest
http://link.springer.com/article/10.1038/s41598-018-21791-2Test

المؤلفون: Li-Ping Hu, Shujia Liang, Xiang He, Hui-Hua Jia, Zhong-Liao Fang, Qing-Li Yang, Qin-Yan Chen, Anna Kramvis, Chao Wang, Kai-Wen Li

المصدر: Virus Research. 227:88-95

مصطلحات موضوعية: Adult, Male, 0301 basic medicine, China, Hepatitis B virus, Cancer Research, Genotype, Combination therapy, HIV Infections, Biology, medicine.disease_cause, 03 medical and health sciences, Liver disease, 0302 clinical medicine, Asian People, Virology, Prevalence, medicine, Humans, Phylogeny, Aged, Sequence Deletion, Hepatitis B Surface Antigens, Coinfection, virus diseases, Cancer, Promoter, Middle Aged, Viral Load, Hepatitis B, medicine.disease, digestive system diseases, CD4 Lymphocyte Count, 030104 developmental biology, Infectious Diseases, Hepatocellular carcinoma, DNA, Viral, Mutation, Immunology, Female, 030211 gastroenterology & hepatology, Viral load

الوصف: In the era of combination therapy for human immunodeficiency virus (HIV), liver disease including hepatocellular carcinoma (HCC), are the major causes of death for patients co-infected with hepatitis B virus (HBV) and HIV. However, the mechanisms remain obscure. We aimed to determine whether HCC-related HBV mutations including 1762T/1764A double mutation and pre-S deletions occur more frequently in HBV/HIV co-infected individuals compared to HBV mono-infected individuals. In this study, the basic core promoter (BCP) and the preS/S regions of HBV isolated from 61 pairs of HBV/HIV co-infected and HBV mono-infected participants were analyzed. We found that the prevalence of HBV isolates with 1762T/1764A and/or preS deletion mutations was 37.7% (95% CI: 29.1-46.3). The prevalence of these mutations in HBV/HIV co-infected group (52.5%, 95% CI: 40.0-65.0) was significantly higher than in the HBV mono-infected group (23.0%, 95% CI: 12.4-33.6) (X2=11.307, P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3fdcd624468cb95341349f3a4b5b007Test
https://doi.org/10.1016/j.virusres.2016.10.002Test

المؤلفون: Lili Chen, Athena P. Kourtis, Liming Wang, Jeffrey Wiener, Fujie Zhang, Marc Bulterys, Colin W. Shepard, Shujia Liang, Wei Liu, Ailing Wang, Linhong Wang, Xiaoyu Wei

المصدر: Journal of Infectious Diseases. 214:1695-1699

مصطلحات موضوعية: Adult, China, Hepatitis B virus, Tenofovir, HIV Infections, medicine.disease_cause, Antiviral Agents, law.invention, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Randomized controlled trial, Pregnancy, law, medicine, Humans, Immunology and Allergy, 030212 general & internal medicine, Pregnancy Complications, Infectious, Coinfection, business.industry, Transmission (medicine), virus diseases, Lamivudine, Viral Load, Hepatitis B, medicine.disease, Virology, digestive system diseases, Clinical trial, Regimen, Treatment Outcome, Infectious Diseases, Female, 030211 gastroenterology & hepatology, business, medicine.drug

الوصف: BACKGROUND There is limited information on antiviral therapy for hepatitis B virus (HBV) infection among pregnant women coinfected with human immunodeficiency virus (HIV) and HBV. METHODS A phase 2 randomized, controlled trial of a regimen containing tenofovir (TDF)/lamivudine (3TC) and a regimen containing 3TC in HIV/HBV-coinfected pregnant women in China. The HBV virological response was compared in study arms. RESULTS The median decline in the HBV DNA level was 2.60 log10 copies/mL in the TDF/3TC arm and 2.24 log10 copies/mL in the 3TC arm (P = .41). All women achieved HBV DNA levels of

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3ea8904e657e30975750ac7c602f850Test
https://doi.org/10.1093/infdis/jiw439Test

المؤلفون: Zhiyong Shen, Shujia Liang, Fan Li, Fuxiong Liang, Yiming Shao, Guangjie Tan, Qiuying Zhu, Yi Feng, He Jiang, Xiaoyi Yang, Runsong Xiong, Lingjie Liao, Zhe Dong, Jianjun Li

المصدر: AIDS Research and Human Retroviruses. 32:689-693

مصطلحات موضوعية: Male, 0301 basic medicine, China, Genotype, Immunology, Population, Human immunodeficiency virus (HIV), HIV Infections, Genome, Viral, Biology, medicine.disease_cause, Genome, Virus, Men who have sex with men, law.invention, 03 medical and health sciences, law, Virology, medicine, Humans, Seroconversion, Heterosexuality, education, Recombination, Genetic, Family Characteristics, education.field_of_study, Sequence Analysis, DNA, 030104 developmental biology, Infectious Diseases, HIV-1, Recombinant DNA, Female

الوصف: In this research, we reported a new second generation recombinant form (GXDY460B) between circulating recombinant form (CRF)01_AE and CRF07_BC in a seroconversion couple who obtained the virus from her husband by heterosexual behavior. The analysis result of the near full-length genomic characterization showed that the genome comprises at least 12 interlaced segments, including six CRF07_BC and six CRF01_AE segments, with CRF07_BC as the main framework. Cocirculation of multiple virus subtypes and multiple infection routes have existed for a long time in Guangxi, but the recombinant strain was rarely reported among heterosexual transmission population because of its lower crowd confounding degree than men who have sex with men and injecting drug user population. It is the first time that the unique recombinant form (URF) between CRF01_AE and CRF07_BC was identified among heterosexual transmission in Guangxi. The emergence of the novel recombinant helps to understand the pattern of the URF virus.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ea27ae34ead3286d087789e72d8862eTest
https://doi.org/10.1089/aid.2015.0329Test