التفاصيل البيبلوغرافية
| العنوان: |
同型半胱氨酸致足细胞凋亡中 FoxO1 DNA 甲基化水平增高. (Chinese) |
| العنوان البديل: |
Increased FoxO1 DNA methylation level in homocysteine-induced podocyte apoptosis. (English) |
| المؤلفون: |
刘 昆, 谢 琳, 曹 军, 丁 宁, 徐灵博, 马胜超, 李桂忠, 姜怡邓, 卢冠军 |
| المصدر: |
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu; Jan2021, Vol. 25 Issue 2, p269-273, 5p |
| مصطلحات موضوعية: |
HOMOCYSTEINE, BAX protein, BCL-2 proteins, DNA methylation, APOPTOSIS, PROTEIN expression |
| الملخص (بالإنجليزية): |
BACKGROUND: The increase of homocysteine can lead to renal injury and podocyte apoptosis, but the specific mechanism is not clear. OBJECTIVE: To investigate the effect of Forkhead box O1 (FoxO1) and its DNA methylation in podocyte apoptosis induced by homocysteine. METHODS: Mouse renal podocytes (MPC-5) were cultured in vitro and divided into control group (0 μmol/L homocysteine) and homocysteine group (80 μmol/L homocysteine). After 48 hours of intervention, the expression of podocyte apoptosis-related proteins Bax, caspase12 and Bcl-2 was detected by immunofluorescence technique; the expression level of FoxO1 mRNA was detected by real-time fluorescence quantitative PCR; the protein expression levels of FoxO1 and DNMT1 were detected by western blot; DNA methylation level of FoxO1 was detected by nested methylation-specific PCR. RESULTS AND CONCLUSION: Compared with the control group, the expression levels of Bax and caspase12 protein in podocytes of the homocysteine group were significantly increased, while the expression of Bcl-2 protein was significantly decreased. The expression levels of FoxO1 mRNA and protein were significantly decreased in the homocysteine group compared with the control group (P < 0.01). At the same time, the methylation level of FoxO1 DNA in the homocysteine group was significantly higher than that in the control group (P < 0.01), and the expression of DNMT1 protein in podocytes in the homocysteine group was significantly higher than that in the control group (P < 0.01). To conclude, FoxO1 DNA hypermethylation plays a significant role in podocyte apoptosis induced by homocysteine, whereas DNMT1 participates in homocysteine-induced podocyte apoptosis. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
背景：同型半胱氨酸增多会引起肾损伤并导致足细胞凋亡，但是其具体机制还尚不清楚。 目的：探讨叉头框转录因子 O1 (forkhead box O，FoxO1) 及其 DNA 甲基化在同型半胱氨酸致足细胞凋亡中的作用。 方法：体外培养小鼠肾脏足细胞 (MPC-5)，将其分为对照组(0 μmol/L同型半胱氨酸)和同型半胱氨酸组 (80 μmol/L 同型半胱氨酸)。干预细胞 48 h后，采用免疫荧光技术检验足细胞凋亡相关蛋白Bax、caspase12 和 Bcl-2 的表达情况；采用实时荧光定量 PCR(qRT-PCR) 检测 FoxO1 mRNA 水平；采用 Western blot 检测 FoxO1和DNMT1蛋白表达水平；采用巢式降落式特异性 PCR(nMS-PCR) 测验 FoxO1 的 DNA甲基化水平。 结果与结论：①与对照组相比，同型半胱氨酸组足细胞中 Bax 和 caspase12 表达明显增高，Bcl-2 表达明显降低；②FoxO1的mRNA和蛋白表达 水平明显降低(P < 0.01)；③与对照组相比，同型半胱氨酸组 FoxO1 DNA 甲基化水平明显升高 (P < 0.01)，同型半胱氨酸组足细胞中DNMT1蛋 白表达明显增高 (P < 0.01)；④结果表明：FoxO1 DNA 高甲基化在同型半胱氨酸致足细胞凋亡中作用显著，而 DNMT1 参与同型半胱氨酸诱导 的足细胞凋亡过程。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
