التفاصيل البيبلوغرافية
| العنوان: |
Phytochemical characterization and toxicological activity attributed to the acetonic extract of South American Vassobia breviflora. |
| المؤلفون: |
Rossato Viana, Altevir1 (AUTHOR) rossato.viana@hotmail.com, Nicola, Isadora2 (AUTHOR), Franco, Camila2 (AUTHOR), Caetano, Patrícia Acosta3 (AUTHOR), Jacob-Lopes, Eduardo3 (AUTHOR), Zepka, Leila Queiroz3 (AUTHOR), Santos, Daniel4 (AUTHOR), Moraes Flores, Erico Marlon4 (AUTHOR), Stefanello Vizzotto, Bruno2 (AUTHOR), Wolf, Katianne5 (AUTHOR), Ferreira Ourique, Aline5 (AUTHOR), Mortari, Sergio Roberto5 (AUTHOR), Bohn Rhoden, Cristiano Rodrigo5,6 (AUTHOR), Fontanari Krause, Luciana Maria7 (AUTHOR) |
| المصدر: |
Journal of Toxicology & Environmental Health: Part A. 2023, Vol. 86 Issue 21, p816-832. 17p. |
| مصطلحات موضوعية: |
*REACTIVE oxygen species, *LACTATE dehydrogenase, *ACETONE, *LUNG cancer, *CERVICAL cancer, *EXTRACTS |
| مصطلحات جغرافية: |
BRAZIL |
| مستخلص: |
The particular plant species found in southern Brazil, Vassobia breviflora (Solanaceae) has only a few apparent studies examining its biological effect. Thus, the aim of the present study was to determine the activity of the acetone extract fraction derived from V. breviflora. Four compounds were identified by ESI-qTOF-MS: eucalrobusone R, aplanoic acid B, pheophorbide A, and pheophytin A. In addition, 5 compounds were identified by HPLC-PDA-MS/MS: all-trans-lutein, 15-cis-lutein, all-trans-β-carotene, 5,8-epoxy-β-carotene, and cis-β-carotene. Cell lines A549 (lung cancer), A375 (melanoma cancer) and HeLa (cervical cancer) were incubated with different concentrations of each studied extract using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH), and 2'−7'dichlorofluorescin diacetate (DCFH-DA) assays. The acetonic extract exhibited cytotoxic activity at a concentration of 0.03 mg/ml in the HeLa strain and 0.1 mg/ml in the others. In addition to increased production of reactive oxygen species (ROS). Antibacterial activity was assessed utilizing minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in 9 ATCCs strains and 7 clinical isolates, as well as determination of biofilm production. Data demonstrated that MIC and MBC were approximately 256 mg/ml in most of the strains tested and antibiofilm effect at S. aureus, S. epidermidis, A. baumannii, and E. faecalis, concentrations below the MIC. Genotoxic activity on plasmid DNA did not produce significant elevated levels in breaks in the isolated genetic material. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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