Effect of cytoglobin overexpression on extracellular matrix component synthesis in human tenon fibroblasts
| العنوان: | Effect of cytoglobin overexpression on extracellular matrix component synthesis in human tenon fibroblasts |
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| المؤلفون: | Ye Qing Wang, Zhuolei Feng, Haiying Wei, Yan You, Xiaomei Zhang, Xiaodan Wang, Yongsheng Hou, Lili Lin |
| المصدر: | Biological Research, Vol 52, Iss 1, Pp 1-6 (2019) Biological Research, Volume: 52, Article number: 23, Published: 13 JUN 2019 Biological Research Biological Research v.52 2019 SciELO Chile CONICYT Chile instacron:CONICYT |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Vascular Endothelial Growth Factor A, 0301 basic medicine, Tenon Capsule, Extracellular matrix component, Cell, Extracellular matrix, 03 medical and health sciences, 0302 clinical medicine, Fibrosis, medicine, Humans, RNA, Messenger, lcsh:QH301-705.5, Messenger RNA, Human tenon fibroblast, biology, Chemistry, Cytoglobin, Glaucoma, Fibroblasts, medicine.disease, Fibronectins, Blot, Fibronectin, 030104 developmental biology, medicine.anatomical_structure, lcsh:Biology (General), 030220 oncology & carcinogenesis, Cancer research, biology.protein, Collagen, Research Article |
| الوصف: | Background Conjunctival filtering bleb scar formation is the main reason for the failure of glaucoma filtration surgery. Cytoglobin (Cygb) has been reported to play an important role in extracellular matrix (ECM) remodeling, fibrosis and tissue damage repairing. This study aimed to investigate the role of Cygb in anti-scarring during excessive conjunctival wound healing after glaucoma filtration surgery. Methods Cygb was overexpressed in human tenon fibroblasts (hTFs) by transfecting hTFs with lentiviral particles encoding pLenti6.2-FLAG-Cygb. Changes in the mRNA and protein levels of fibronectin, collagen I, collagen III, TGF-β1, and HIF1α were determined by RT-PCR and western blotting respectively. Results After Cygb overexpression, hTFs displayed no significant changes in visual appearance and cell counts compared to controls. Whereas, Cygb overexpression significantly decreased the mRNA and protein expression levels of collagen I, collagen III and fibronectin compared with control (p |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 0717-6287 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f41b01c25fd48b69a270cd5ac24b26a7Test http://link.springer.com/article/10.1186/s40659-019-0229-4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f41b01c25fd48b69a270cd5ac24b26a7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 07176287 |
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