التفاصيل البيبلوغرافية
| العنوان: |
Measurement of estrogenic activity in sediments from Haihe and Dagu River, China |
| المؤلفون: |
Song, Maoyong, Xu, Yan, Jiang, Qinting, Lam, Paul K. S., O'Toole, Desmond K., Giesy, John P., Jiang, Guibin |
| سنة النشر: |
2006 |
| المجموعة: |
Research Center for Eco-Environmental Sciences: RCEES OpenIR (Chinese Academy of Sciences) / 中国科学院生态环境研究中心机构知识库 |
| مصطلحات موضوعية: |
Estrogenic Activity, Sediments, E2-eqs, Haihe And Dagu Rivers, In Vitro Bioassay, Mcf-7 Cell Line |
| الوصف: |
Sediments from two rivers in China, the Haihe and Dagu Rivers, were examined for estrogenic activity using an estrogen receptor (ER)mediated in vitro bioassay system. ER-active compounds were isolated from sediments by Soxhlet extraction, and the crude extracts were fractionated using a florisil colunm into three fractions. The estrogenic activity of each extract was detected by measuring luciferase activity in the human breast cancer cell line MCF-7 transfected with a luciferase receptor gene. Significant estrogenic activity was observed in each total extract. The 17 beta-estradiol equivalents (E2-EQs) ranged from 8.24 to 95.28 ng E2 g(-1) dw. As a result, the relative estrogenic potencies of three fractions in this study descended in the order of Fraction 3 > Fraction2 > Fraction1. The results of the bioassay analysis indicated the heavy pollution status of these sites with estrogenic contaminants. In this study, five selected chemicals, the natural estrogens 17 beta-estradiol (E2) and estrone (E1), and the xeno-estrogens 4-octylphenol (OP), 4-nonylphenol (NP), and Bisphenol A (BPA) were also analyzed using the in vitro bioassay. The estrogenic activity of these chemicals were E2 > E1 > NP > OP > BPA. (c) 2006 Elsevier Ltd. All rights reserved. |
| نوع الوثيقة: |
report |
| اللغة: |
unknown |
| العلاقة: |
ENVIRONMENT INTERNATIONAL; http://ir.rcees.ac.cn/handle/311016/22890Test |
| الإتاحة: |
http://ir.rcees.ac.cn/handle/311016/22890Test |
| حقوق: |
cn.org.cspace.api.content.CopyrightPolicy@cebcaf |
| رقم الانضمام: |
edsbas.71D332F3 |
| قاعدة البيانات: |
BASE |
