دورية أكاديمية
Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates
| العنوان: | Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates |
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| المؤلفون: | Encinas, Micaela, Marfil, Maria Jimena, Garbaccio, Sergio Gabriel, Barandiaran, Soledad, Huertas, Pablo Sebastián, Morsella, C., Macías, A., Magnano, Gabriel Gustavo, Zapata, L., Bigi, Fabiana, Cataldi, Ángel Adrián, Paolicchi, Fernando Alberto, Zumárraga, Martín José, Eirin, Maria Emilia |
| بيانات النشر: | Churchill Livingstone |
| المجموعة: | CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| مصطلحات موضوعية: | BOVINE TUBERCULOSIS, CFP-10, ESAT-6, ESPC, FIELD ISOLATES, MYCOBACTERIUM BOVIS, POLYMORPHISM, https://purl.org/becyt/ford/4.3Test, https://purl.org/becyt/ford/4Test |
| الوصف: | ESAT-6, CFP-10 and EspC are virulence factors that have been extensively assayed for bovine and human tuberculosis diagnosis due their potent T-cell inducing activities. While polymorphisms of ESAT-6 and CFP-10 were analyzed, with the description of CFP-10 variants in M. tuberculosis, this fact has not been explored in M. bovis field isolates. The coding sequences of esxA (ESAT-6), esxB (CFP-10) and mb3645c (EspC) from 58 M. bovis strains exhibiting genomic variability (spoligotyping) were analyzed. Two genes –esxA and esxB – remained invariant while mb3645c exhibited one synonymous polymorphism (G to A mutation, position 66bp) in one isolate, compared to M. bovis AF2122/97 reference strain. All isolates exhibited a synonymous nucleotide polymorphism simultaneously (G to A mutation, position 255bp), compared to M. tuberculosis H37Rv reference strain. This study confirms the high conservation for ESAT-6, CFP-10 and EspC in local M. bovis field isolates and reinforce the use of these three antigens in the diagnosis of bovine tuberculosis. Further studies should be performed to globally confirm these findings. ; Fil: Encinas, Micaela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Marfil, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina ; Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina ; Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1472-9792 |
| العلاقة: | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1472979217304924Test; http://hdl.handle.net/11336/97548Test; Encinas, Micaela; Marfil, Maria Jimena; Garbaccio, Sergio Gabriel; Barandiaran, Soledad; Huertas, Pablo Sebastián; et al.; Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates; Churchill Livingstone; Tuberculosis (Edinb); 111; 7-2018; 143-146; CONICET Digital; CONICET |
| الإتاحة: | https://doi.org/10.1016/j.tube.2018.06.007Test http://hdl.handle.net/11336/97548Test |
| حقوق: | info:eu-repo/semantics/restrictedAccess ; https://creativecommons.org/licenses/by-nc-sa/2.5/arTest/ |
| رقم الانضمام: | edsbas.37572739 |
| قاعدة البيانات: | BASE |
| تدمد: | 14729792 |
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