دورية أكاديمية
Production of Mycobacterium bovis Antigens Included in Recombinant Occlusion Bodies of Baculovirus
| العنوان: | Production of Mycobacterium bovis Antigens Included in Recombinant Occlusion Bodies of Baculovirus |
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| المؤلفون: | Villafañe, Luciana María, Forrellad, Marina Andrea, López, María Gabriela, Garbaccio, Sergio Gabriel, Garro, Carlos, Rocha, Rosana Valeria, Eirin, Maria Emilia, Singh, Mahavir, Taboga, Oscar Alberto, Bigi, Fabiana |
| بيانات النشر: | Karger |
| المجموعة: | CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| مصطلحات موضوعية: | ESAT-6, CFP10, RV3615c, MYCOBACTERIUM BOVIS, OCCLUSION BODIES, BACULOVIRUS, IFN-γ ASSAY, https://purl.org/becyt/ford/1.6Test, https://purl.org/becyt/ford/1Test |
| الوصف: | Bovine tuberculosis (bTB) is a disease produced by Mycobacterium bovis that affects livestock, wild animals, and humans. The classical diagnostic method to detect bTB is measuring the response induced with the intradermal injection of purified protein derivative of M. bovis (PPDb). Another ancillary bTB test detects IFN-γproduced in whole blood upon stimulation with PPDb, protein/peptide cocktails, or individual antigens. Among the most used M. bovis antigens in IFN-γassays are the secreted proteins ESAT-6 and CFP-10, which together with antigen Rv3615c improve the sensitivity of the test in comparison to PPDb. Protein reagents for immune stimulation are generally obtained from Escherichia coli, because this bacterium produces a high level of recombinant proteins. However, E. coli recombinant antigens are in general contaminated with lipopolysaccharides and other components that produce non-specific IFN-γsecretion in in vitro assays. In this work, we produced the relevant ESAT-6, CFP-10, and Rv3615c M. bovis antigens as fusions to the polyhedrin protein from the baculovirus AcMNPV. We obtained chimeric proteins effectively incorporated to the occlusion bodies and easily purified the recombinant polyhedra with no reactive contaminants. In an IFN-γassay, these fusion proteins showed equivalent sensibility but better specificity than the same M. bovis proteins produced in E. coli. ; Fil: Villafañe, Luciana María. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina ; Fil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1464-1801 |
| العلاقة: | info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/FullText/506687Test; http://hdl.handle.net/11336/142408Test; Villafañe, Luciana María; Forrellad, Marina Andrea; López, María Gabriela; Garbaccio, Sergio Gabriel; Garro, Carlos; et al.; Production of Mycobacterium bovis Antigens Included in Recombinant Occlusion Bodies of Baculovirus; Karger; Journal of Molecular Microbiology and Biotechnology; 29; 1-6; 7-2020; 83-90; CONICET Digital; CONICET |
| الإتاحة: | https://doi.org/10.1159/000506687Test http://hdl.handle.net/11336/142408Test |
| حقوق: | info:eu-repo/semantics/restrictedAccess ; https://creativecommons.org/licenses/by-nc-sa/2.5/arTest/ |
| رقم الانضمام: | edsbas.5BCFC230 |
| قاعدة البيانات: | BASE |
| تدمد: | 14641801 |
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