Development of a 135K SNP genotyping array for Actinidia arguta and its applications for genetic mapping and QTL analysis in kiwifruit
| العنوان: | Development of a |
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| المؤلفون: | Ran Wang, Siyuan Xing, Peter M. Bourke, Xiuquan Qi, Miaomiao Lin, Danny Esselink, Paul Arens, Roeland E. Voorrips, Richard G.F. Visser, Leiming Sun, Yunpeng Zhong, Hong Gu, Yukuo Li, Sikai Li, Chris Maliepaard, Jinbao Fang |
| المصدر: | Plant Biotechnology Journal 21 (2023) 2 Plant Biotechnology Journal, 21(2), 369-380 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | QTL analysis, Plant Science, Animal Breeding and Genomics, PE&RC, Plant Breeding, Laboratorium voor Plantenveredeling, kiwifruit, genetic linkage map, Fokkerij en Genomica, EPS, SNP array, polyploid genetics, Agronomy and Crop Science, Biotechnology |
| الوصف: | Kiwifruit (Actinidia spp) is a woody, perennial and deciduous vine. In this genus, there are multiple ploidy levels but the main cultivated cultivars are polyploid. Despite the availability of many genomic resources in kiwifruit, SNP genotyping is still a challenge given these different levels of polyploidy. Recent advances in SNP array technologies have offered a high-throughput genotyping platform for genome-wide DNA polymorphisms. In this study, we developed a high-density SNP genotyping array to facilitate genetic studies and breeding applications in kiwifruit. SNP discovery was performed by genome-wide DNA sequencing of 40 kiwifruit genotypes. The identified SNPs were stringently filtered for sequence quality, predicted conversion performance and distribution over the available Actinidia chinensis genome. A total of 134 729 unique SNPs were put on the array. The array was evaluated by genotyping 400 kiwifruit individuals. We performed a multidimensional scaling analysis to assess the diversity of kiwifruit germplasm, showing that the array was effective to distinguish kiwifruit accessions. Using a tetraploid F1 population, we constructed an integrated linkage map covering 3060.9 cM across 29 linkage groups and performed QTL analysis for the sex locus that has been identified on Linkage Group 3 (LG3) in Actinidia arguta. Finally, our dataset presented evidence of tetrasomic inheritance with partial preferential pairing in A. arguta. In conclusion, we developed and evaluated a 135K SNP genotyping array for kiwifruit. It has the advantage of a comprehensive design that can be an effective tool in genetic studies and breeding applications in this high-value crop. |
| وصف الملف: | application/pdf |
| تدمد: | 1467-7652 1467-7644 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b92a9ff51be1b0b3a169e76be7187eaTest https://doi.org/10.1111/pbi.13958Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3b92a9ff51be1b0b3a169e76be7187ea |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14677652 14677644 |
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