Cytoprotective Effects of Natural Highly Bio-Available Vegetable Derivatives on Human-Derived Retinal Cells
| العنوان: | Cytoprotective Effects of Natural Highly Bio-Available Vegetable Derivatives on Human-Derived Retinal Cells |
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| المؤلفون: | Munia, Ingrid, Gafray, Laurent, Bringer, Marie-Agnès, Goldschmidt, Pablo, Proukhnitzky, Lil, Jacquemot, Nathalie, Cercy, Christine, Ramchani Ben Otman, Khaoula, Errera, Marie Hélène, Ranchon-Cole, Isabelle |
| المساهمون: | Neuro-Dol (Neuro-Dol), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts (CHNO), MDPI AG, Julien, Sabine |
| المصدر: | Nutrients Nutrients, MDPI, 2020, 12 (3), pp.879. ⟨10.3390/nu12030879⟩ Nutrients, 2020, 12 (3), pp.879. ⟨10.3390/nu12030879⟩ Volume 12 Issue 3 Nutrients, Vol 12, Iss 3, p 879 (2020) |
| بيانات النشر: | HAL CCSD, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | autophagy, retina, antioxidant, Cell Survival, [SDV]Life Sciences [q-bio], lcsh:TX341-641, Retinal Pigment Epithelium, resveratrol, Protective Agents, Article, Vegetables, Humans, Cells, Cultured, Biological Products, lutein, Dose-Response Relationship, Drug, Plant Extracts, protection curcumin, apoptosis, food and beverages, Epithelial Cells, eye diseases, [SDV] Life Sciences [q-bio], [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, Oxidative Stress, Cytoprotection, sense organs, Reactive Oxygen Species, lcsh:Nutrition. Foods and food supply, damage, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| الوصف: | This article belongs to the Section Micronutrients and Human Health.; International audience; Retinal pigment epithelial cells are crucial for retina maintenance, making their cytoprotection an excellent way to prevent or slow down retinal degeneration. In addition, oxidative stress, inflammation, apoptosis, neovascularization, and/or autophagy are key pathways involved in degenerative mechanisms. Therefore, here we studied the effects of curcumin, lutein, and/or resveratrol on human retinal pigment epithelial cells (ARPE-19). Cells were incubated with individual or combined agent(s) before induction of (a) H2O2-induced oxidative stress, (b) staurosporin-induced apoptosis, (c) CoCl2-induced hypoxia, or (d) a LED-autophagy perturbator. Metabolic activity, cellular survival, caspase 3/7 activity (casp3/7), cell morphology, VEGF levels, and autophagy process were assessed. H2O2 provoked a reduction in cell survival, whereas curcumin reduced metabolic activity which was not associated with cell death. Cell death induced by H2O2 was significantly reduced after pre-treatment with curcumin and lutein, but not resveratrol. Staurosporin increased caspase-3/7 activity (689%) and decreased cell survival by 32%. Curcumin or lutein protected cells from death induced by staurosporin. Curcumin, lutein, and resveratrol were ineffective on the increase of caspase 3/7 induced by staurosporin. Pre-treatment with curcumin or lutein prevented LED-induced blockage of autophagy flux. Basal-VEGF release was significantly reduced by lutein. Therefore, lutein and curcumin showed beneficial protective effects on human-derived retinal cells against several insults. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2072-6643 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::8d19f9b73cdd6c9d9630d584cf630eacTest https://hal.inrae.fr/hal-02865916Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....8d19f9b73cdd6c9d9630d584cf630eac |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20726643 |
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