تقرير
Transcriptomic diversity in human medullary thymic epithelial cells
| العنوان: | Transcriptomic diversity in human medullary thymic epithelial cells |
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| المؤلفون: | Carter, Jason A, Strömich, Léonie, Peacey, Matthew, Chapin, Sarah R, Velten, Lars, Steinmetz, Lars M, Brors, Benedikt, Pinto, Sheena, Meyer, Hannah V |
| سنة النشر: | 2022 |
| المجموعة: | Cold Spring Harbor Laboratory: CSHL Institutional Repository |
| مصطلحات موضوعية: | epithelial cell, epithelial cells, transcriptomes |
| الوصف: | The induction of central T cell tolerance in the thymus depends on the presentation of peripheral self-epitopes by medullary thymic epithelial cells (mTECs). This promiscuous gene expression (pGE) drives mTEC transcriptomic diversity, with non-canonical transcript initiation, alternative splicing, and expression of endogenous retroelements (EREs) representing important but incompletely understood contributors. Here we map the expression of genome-wide transcripts in immature and mature human mTECs using high-throughput 5' cap and RNA sequencing. Both mTEC populations show high splicing entropy, potentially driven by the expression of peripheral splicing factors. During mTEC maturation, rates of global transcript mis-initiation increase and EREs enriched in long terminal repeat retrotransposons are up-regulated, the latter often found in proximity to differentially expressed genes. As a resource, we provide an interactive public interface for exploring mTEC transcriptomic diversity. Our findings therefore help construct a map of transcriptomic diversity in the healthy human thymus and may ultimately facilitate the identification of those epitopes which contribute to autoimmunity and immune recognition of tumor antigens. |
| نوع الوثيقة: | report |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | http://repository.cshl.edu/id/eprint/40698/1/Transcriptomic%20diversity%20in%20human%20medullary%20thymic%20epithelial%20cells.pdfTest; Carter, Jason A, Strömich, Léonie, Peacey, Matthew, Chapin, Sarah R, Velten, Lars, Steinmetz, Lars M, Brors, Benedikt, Pinto, Sheena, Meyer, Hannah V (August 2022) Transcriptomic diversity in human medullary thymic epithelial cells. Nature Communications, 13 (1). p. 4296. ISSN 2041-1723 |
| DOI: | 10.1038/s41467-022-31750-1 |
| الإتاحة: | https://doi.org/10.1038/s41467-022-31750-1Test http://repository.cshl.edu/id/eprint/40698Test/ http://repository.cshl.edu/id/eprint/40698/1/Transcriptomic%20diversity%20in%20human%20medullary%20thymic%20epithelial%20cells.pdfTest https://www.ncbi.nlm.nih.gov/pubmed/35918316Test |
| حقوق: | cc_by |
| رقم الانضمام: | edsbas.677C4546 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-022-31750-1 |
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