دورية أكاديمية
Transcriptional Programming of Normal and Inflamed Human Epidermis at Single-Cell Resolution
| العنوان: | Transcriptional Programming of Normal and Inflamed Human Epidermis at Single-Cell Resolution |
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| المؤلفون: | Cheng, Jeffrey B., Sedgewick, Andrew J., Finnegan, Alex I., Harirchian, Paymann, Lee, Jerry, Kwon, Sunjong, Fassett, Marlys S., Golovato, Justin, Gray, Matthew, Ghadially, Ruby, Liao, Wilson, Perez White, Bethany E., Mauro, Theodora M., Mully, Thaddeus, Kim, Esther A., Sbitany, Hani, Neuhaus, Isaac M., Grekin, Roy C., Yu, Siegrid S., Gray, Joe W., Purdom, Elizabeth, Paus, Ralf, Vaske, Charles J., Benz, Stephen C., Song, Jun S., Cho, Raymond J. |
| المصدر: | Cheng , J B , Sedgewick , A J , Finnegan , A I , Harirchian , P , Lee , J , Kwon , S , Fassett , M S , Golovato , J , Gray , M , Ghadially , R , Liao , W , Perez White , B E , Mauro , T M , Mully , T , Kim , E A , Sbitany , H , Neuhaus , I M , Grekin , R C , Yu , S S , Gray , J W , Purdom , E , Paus , R .... |
| سنة النشر: | 2018 |
| المجموعة: | The University of Manchester: Research Explorer - Publications |
| مصطلحات موضوعية: | epidermis, keratinocyte, single-cell RNA-seq, skin, ResearchInstitutes_Networks_Beacons/lydia_becker_institute_of_immunology_and_inflammation, name=Lydia Becker Institute |
| الوصف: | Perturbations in the transcriptional programs specifying epidermal differentiation cause diverse skin pathologies ranging from impaired barrier function to inflammatory skin disease. However, the global scope and organization of this complex cellular program remain undefined. Here we report single-cell RNA sequencing profiles of 92,889 human epidermal cells from 9 normal and 3 inflamed skin samples. Transcriptomics-derived keratinocyte subpopulations reflect classic epidermal strata but also sharply compartmentalize epithelial functions such as cell-cell communication, inflammation, and WNT pathway modulation. In keratinocytes, ∼12% of assessed transcript expression varies in coordinate patterns, revealing undescribed gene expression programs governing epidermal homeostasis. We also identify molecular fingerprints of inflammatory skin states, including S100 activation in the interfollicular epidermis of normal scalp, enrichment of a CD1C + CD301A + myeloid dendritic cell population in psoriatic epidermis, and IL1β hi CCL3 hi CD14 + monocyte-derived macrophages enriched in foreskin. This compendium of RNA profiles provides a critical step toward elucidating epidermal diseases of development, differentiation, and inflammation. Cheng et al. report single-cell RNA sequencing of normal and inflamed human epidermis, revealing a discrete set of specialized keratinocytes that exhibit a distinct composition at different anatomic sites. Myeloid dendritic cells and macrophages also vary sharply with epidermal anatomic site and inflammation, indicating dynamic programming of antigen-presenting cells. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://research.manchester.ac.uk/en/publications/fa7e0902-f0b5-4cff-8969-bbb18d708878Test |
| DOI: | 10.1016/j.celrep.2018.09.006 |
| الإتاحة: | https://doi.org/10.1016/j.celrep.2018.09.006Test https://research.manchester.ac.uk/en/publications/fa7e0902-f0b5-4cff-8969-bbb18d708878Test http://www.scopus.com/inward/record.url?scp=85054767561&partnerID=8YFLogxKTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.A2D80516 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.celrep.2018.09.006 |
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