التفاصيل البيبلوغرافية
| العنوان: |
Matrix elasticity regulates mesenchymal stem cell chemotaxis. |
| المؤلفون: |
Saxena, Neha1, Mogha, Pankaj1, Dash, Silalipi1, Majumder, Abhijit1, Jadhav, Sameer1 srjadhav@iitb.ac.in, Sen, Shamik2 shamiks@iitb.ac.in |
| المصدر: |
Journal of Cell Science. 3/15/2018, Vol. 131 Issue 6, p1-48. 48p. |
| مصطلحات موضوعية: |
*MESENCHYMAL stem cells, *EPIDERMAL growth factor, *CHEMOTAXIS |
| مستخلص: |
Efficient homing of human mesenchymal stem cells (hMSCs) is likely to be dictated by the combination of physical and chemical factors present in the microenvironment. However, the crosstalk between the physical and chemical cues remains incompletely understood. Here, we address this question by probing the efficiency of epidermal growth factor (EGF)-induced hMSC chemotaxis on substrates of varying stiffness (3, 30 and 600 kPa, respectively) inside a PDMS microfluidic device. Chemotactic speed was found to be the sum of a stiffnessdependent component and a chemotactic component. While the stiffness-dependent component scaled inversely with stiffness, the chemotactic component was stiffnessindependent. Faster chemotaxis on the softest 3 kPa substrates is attributed to a combination of weaker adhesions and higher protrusion rate. While chemotaxis was mildly sensitive to contractility inhibitors, suppression of chemotaxis by the actin depolymerizing drug demonstrates the role of actin-mediated protrusions in driving chemotaxis. In addition to highlighting the collective influence of physical and chemical cues in chemotactic migration, our results suggest that hMSC homing is more efficient on softer substrates. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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