التفاصيل البيبلوغرافية
| العنوان: |
Genomic analysis of Neisseria meningitidis carriage isolates during an outbreak of serogroup C clonal complex 11, Tuscany, Italy. |
| المؤلفون: |
Ambrosio, Luigina, Neri, Arianna, Fazio, Cecilia, Rossolini, Gian Maria, Vacca, Paola, Riccobono, Eleonora, Voller, Fabio, Miglietta, Alessandro, Stefanelli, Paola |
| المصدر: |
PLoS ONE; 5/28/2019, Vol. 14 Issue 5, p1-12, 12p |
| مصطلحات موضوعية: |
MENINGOCOCCAL infections, NEISSERIA, NEISSERIA meningitidis, COMMUNICABLE diseases, ENVIRONMENTAL chemistry, MEDICAL microbiology, PHYSICAL sciences |
| مصطلحات جغرافية: |
ITALY, TUSCANY (Italy) |
| مستخلص: |
Background: In 2015–2016, a cross-sectional carriage survey was performed in Tuscany Region, Italy, during an outbreak of invasive meningococcal disease due to Neisseria meningitidis serogroup C clonal complex 11 (MenC:cc11). This study aims to evaluate the genomic profile of meningococcal carriage isolates collected during the survey. Methods: Whole-genome sequencing (WGS) was performed using Illumina MiSeq on 85 cultivated meningococcal carriage isolates received at the Dept. of Infectious Disease, National Institute of Health (Istituto Superiore di Sanità, ISS), as National Reference Laboratory (NRL) for Invasive Meningococcal Disease (IMD). De novo assembled genomes were scanned by the BIGSdb platform to assign: the genotypic profiles, the cgMLST, the vaccine antigen variants and allele types of antimicrobial resistance associated genes, together with denitrification pathway loci. Results: Capsule null and non-groupable meningococci accounted for 52.9% and 10.6%, respectively. Among the remaining carriage isolates, serogroup B was the predominant (71.0%). Serogroup C meningococci were culture negative and unavailable for WGS. Overall, 64 genotypic profiles were identified and, based on cgMLST, isolates clustered according to clonal complexes. Eight isolates (9.4%) harbored at least one gene encoding a 4CMenB vaccine antigen. Mutated penA alleles were found in more than 82%. Finally, complete aniA and norB coding sequences were detected among 71.8% of carriage isolates. Conclusions: Meningococcal carriage isolates collected during the MenC:cc11 outbreak were characterized by an extensive genetic diversity. The lack of outbreak-related isolates among carriage might be attributable to the high transmissibility with low duration of colonization of MenC:cc11 meningococci. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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